##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063984_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1424354
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.238206934512068	32.0	32.0	32.0	32.0	32.0
2	30.783693520009773	32.0	32.0	32.0	32.0	32.0
3	30.805993453874528	32.0	32.0	32.0	32.0	32.0
4	30.84386886967706	32.0	32.0	32.0	32.0	32.0
5	30.723388988973248	32.0	32.0	32.0	32.0	32.0
6	34.39253444017428	36.0	36.0	36.0	32.0	36.0
7	34.328166312588024	36.0	36.0	36.0	32.0	36.0
8	34.28696377445495	36.0	36.0	36.0	32.0	36.0
9	34.443455068051904	36.0	36.0	36.0	32.0	36.0
10	34.090254950665354	36.0	36.0	36.0	32.0	36.0
11	34.42128782591968	36.0	36.0	36.0	32.0	36.0
12	34.209041432115896	36.0	36.0	36.0	32.0	36.0
13	34.30664638144731	36.0	36.0	36.0	32.0	36.0
14	34.18622828313748	36.0	36.0	36.0	32.0	36.0
15	34.1112567521838	36.0	36.0	36.0	32.0	36.0
16	34.12403587872116	36.0	36.0	36.0	32.0	36.0
17	34.03588644395986	36.0	36.0	36.0	32.0	36.0
18	34.03783258937034	36.0	36.0	36.0	32.0	36.0
19	34.04591765811027	36.0	36.0	36.0	32.0	36.0
20	34.02036572368947	36.0	36.0	36.0	32.0	36.0
21	34.00665986124236	36.0	36.0	36.0	32.0	36.0
22	33.98497143266351	36.0	36.0	36.0	32.0	36.0
23	33.92623603401963	36.0	36.0	36.0	32.0	36.0
24	33.900425736860356	36.0	36.0	36.0	32.0	36.0
25	33.50040018141558	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	17.0
5	15.0
6	56.0
7	20.0
8	64.0
9	90.0
10	129.0
11	53.0
12	88.0
13	66.0
14	141.0
15	258.0
16	386.0
17	529.0
18	683.0
19	972.0
20	1484.0
21	2180.0
22	3553.0
23	5533.0
24	8197.0
25	12029.0
26	16808.0
27	21862.0
28	30142.0
29	40285.0
30	53302.0
31	72176.0
32	102014.0
33	143732.0
34	300421.0
35	607066.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.90582239646911	16.8655506687138	10.972145938481983	26.256480996335103
2	16.80686547023177	19.511587201175043	37.72385762139232	25.957689707200863
3	18.988577085366455	23.828185883889265	28.347293113253247	28.83594391749103
4	12.492461203721971	15.100551218529256	35.6974202748159	36.70956730293288
5	14.518222995512623	36.63318160083751	33.54573074187431	15.302864661775558
6	34.39590894696671	35.40568228336146	16.77855553472487	13.419853234946963
7	30.451642079480468	30.161272653730776	20.83953630776491	18.547548959023853
8	28.234036561101732	33.11245534156025	19.261965512131436	19.391542585206583
9	27.850640593391773	13.573836817262306	18.270678804225668	40.304843785120255
10	16.15191221438662	26.360551266227816	31.540034519117228	25.94750200026834
11	37.70270934749471	20.399623487099518	22.77748509051061	19.12018207489516
12	25.497740401536234	23.079587181582077	28.905297780163163	22.51737463671853
13	30.096469437762742	19.271055796596453	25.007376413461323	25.62509835217948
14	23.70820989003037	19.759686889003948	24.85539833341881	31.67670488754687
15	25.196149574543863	27.79679543332741	21.856355780098923	25.150699212029803
16	25.599370525709386	25.158808776108078	24.375127335061578	24.866693363120955
17	23.964483323544865	25.720787048875827	25.23555545564139	25.079174171937918
18	24.84398515522792	24.34941704338344	26.801038740583248	24.00555906080539
19	26.141657819873913	24.22210871472905	25.02207892486702	24.61415454053002
20	26.374476462689994	23.975251027401352	24.743773234657837	24.90649927525082
21	27.142614247935093	23.579225297686797	24.00696124802221	25.2711992063559
22	26.573261976478097	24.26983233668407	24.50281710625328	24.654088580584556
23	24.45422364499207	24.11161591959539	25.496661752864313	25.937498682548227
24	25.47253726267746	24.415467436347463	25.29491861721636	24.817076683758714
25	25.00312665554655	24.37393114050558	25.288319798545018	25.33462240540286
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	100.0
1	100.0
2	288.5
3	477.0
4	477.0
5	477.0
6	1390.0
7	2303.0
8	2303.0
9	2303.0
10	2347.5
11	2392.0
12	2392.0
13	2392.0
14	2543.5
15	2695.0
16	2695.0
17	2695.0
18	4952.0
19	7209.0
20	7209.0
21	7209.0
22	12425.0
23	17641.0
24	17641.0
25	17641.0
26	28211.0
27	38781.0
28	38781.0
29	38781.0
30	50148.0
31	61515.0
32	61515.0
33	61515.0
34	75566.5
35	89618.0
36	89618.0
37	89618.0
38	102071.0
39	114524.0
40	114524.0
41	114524.0
42	131360.0
43	148196.0
44	148196.0
45	148196.0
46	166715.5
47	185235.0
48	185235.0
49	185235.0
50	189560.5
51	193886.0
52	193886.0
53	193886.0
54	178851.0
55	163816.0
56	163816.0
57	163816.0
58	151730.0
59	139644.0
60	139644.0
61	139644.0
62	123654.0
63	107664.0
64	107664.0
65	107664.0
66	89503.5
67	71343.0
68	71343.0
69	71343.0
70	54068.5
71	36794.0
72	36794.0
73	36794.0
74	28293.5
75	19793.0
76	19793.0
77	19793.0
78	15680.0
79	11567.0
80	11567.0
81	11567.0
82	7964.5
83	4362.0
84	4362.0
85	4362.0
86	3398.5
87	2435.0
88	2435.0
89	2435.0
90	1749.5
91	1064.0
92	1064.0
93	1064.0
94	736.5
95	409.0
96	409.0
97	409.0
98	650.0
99	891.0
100	891.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04043938515284824
2	0.0047038868146542226
3	0.0016849743813686763
4	0.003861399623969884
5	0.009618395426979528
6	0.012145856999032544
7	0.021483423362450627
8	0.03447176755216751
9	0.047319697210103664
10	0.05581477638283741
11	0.053638351140236205
12	0.06115052859050489
13	0.06304612476954466
14	0.06746918252063742
15	0.057780579827767534
16	0.06627565900050128
17	0.06388861196022899
18	0.0766663343522748
19	0.07378783645076996
20	0.07743861427706876
21	0.07343680012131816
22	0.07617488349104225
23	0.08087877030569648
24	0.07582384716159045
25	0.07778965060652057
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1424354.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.55392540736137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.48561278312418	36.16568886993414
2	14.413580852935414	13.996718591727964
3	4.78208306072646	6.965667126669568
4	2.1068817234026715	4.091895121609051
5	1.0941161900803502	2.656181794007387
6	0.6974531969787817	2.031845430074009
7	0.4550369953008556	1.5465682629199313
8	0.3120499267348156	1.2120999092843854
9	0.23732800669910603	1.0370885700911538
>10	1.2136804212529801	10.456241670452874
>50	0.09033583956642109	3.0499741978992265
>100	0.09528276211008925	9.754890791242264
>500	0.01191031376494408	3.891344243805444
>1k	0.004647927322905008	3.1437954202827045
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3599	0.2526759499394111	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2575	0.18078370966768093	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1713	0.12026504647018929	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1638	0.11499950152841218	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1520	0.10671504415334951	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1498	0.10517048430376157	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1454	0.10208136460458565	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1426	0.10011556115965554	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.2124359534216914E-4
2	0.0	0.0	0.0	0.0	4.2124359534216914E-4
3	0.0	0.0	0.0	0.0	4.2124359534216914E-4
4	0.0	0.0	0.0	0.0	4.2124359534216914E-4
5	0.0	7.020726589036153E-5	0.0	0.0	4.2124359534216914E-4
6	0.0	7.020726589036153E-5	0.0	0.0	6.318653930132537E-4
7	0.0	7.020726589036153E-5	0.0	0.0	6.318653930132537E-4
8	0.0	7.020726589036153E-5	0.0	0.0	6.318653930132537E-4
9	0.0	7.020726589036153E-5	0.0	0.0	6.318653930132537E-4
10	0.0	7.020726589036153E-5	0.0	0.0	6.318653930132537E-4
11	0.0	7.020726589036153E-5	0.0	0.0	7.020726589036152E-4
12	0.0	7.020726589036153E-5	0.0	1.4041453178072305E-4	0.0014041453178072305
13	0.0	1.4041453178072305E-4	0.0	1.4041453178072305E-4	0.0015445598495879536
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAA	85	1.0186341E-9	15.648101	10
CGTTATT	110	0.0	15.538846	2
TCGCGTA	90	2.2846507E-9	14.778761	9
GCGTAAC	90	2.2864697E-9	14.778242	11
ATAATAC	80	2.0030948E-6	13.060158	3
CGTCGTA	270	0.0	13.019385	10
TAGAGTG	180	0.0	12.66573	5
GCGTTAT	135	2.7284841E-11	12.663506	1
AACGTCT	115	5.322363E-9	12.391259	6
CGAACGA	170	0.0	12.295368	16
CGAACGT	125	1.4115358E-9	12.158674	4
TCGAACG	125	1.4133548E-9	12.157819	3
GCCGTTA	55	0.0030658913	12.092139	15
GCATCGC	220	0.0	12.092139	14
CGCATCG	220	0.0	12.091289	13
AATCGAA	95	1.038281E-6	12.001221	7
CGGTCCA	350	0.0	11.943652	10
GTCGTAG	295	0.0	11.915629	11
ACGTCTG	120	9.993528E-9	11.876207	7
TATACTC	80	2.8681336E-5	11.874122	5
>>END_MODULE