FastQCFastQC Report
Thu 2 Feb 2017
SRR4063984_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063984_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424354
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17010.11942255927950496No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15050.10566193516499409No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14520.10194095007280493No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC14290.10032618295732663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28650.020.9642871
GTATAGA1800.019.5564291
ACCGTCG3950.018.9362268
ATACGCA708.119937E-418.85864825
CGTAAGG708.127607E-418.8559992
GTCCTAA5500.018.8008381
CATAACG600.00741532718.3322242
GGCGTTA4600.018.1734542
TGTAGGA11950.018.0407472
GTAGGAC11200.017.8739153
ATACCGT4500.017.5995486
TAGGACG10600.017.4335174
TACCGTC4550.017.4061497
GTCTTAC1651.6589183E-917.3341081
TAGGACA5300.017.0184354
GATTTCG4400.016.99956741
TGTACTA1307.320996E-716.9226445
TTAGGAC7000.016.6561343
CCGTCGT4450.016.3141929
AACGCAG36150.016.3094046
Produced by FastQC (version 0.11.5)