##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063984_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1424354
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.317318587935304	32.0	32.0	32.0	32.0	32.0
2	31.425677184183144	32.0	32.0	32.0	32.0	32.0
3	31.498599365045486	32.0	32.0	32.0	32.0	32.0
4	31.57662561413806	32.0	32.0	32.0	32.0	32.0
5	31.527698170539065	32.0	32.0	32.0	32.0	32.0
6	35.12515849290275	36.0	36.0	36.0	36.0	36.0
7	35.13902161962546	36.0	36.0	36.0	36.0	36.0
8	35.08550051461926	36.0	36.0	36.0	36.0	36.0
9	35.195990603459535	36.0	36.0	36.0	36.0	36.0
10	35.05215978612058	36.0	36.0	36.0	36.0	36.0
11	35.1882642938483	36.0	36.0	36.0	36.0	36.0
12	35.09887570084403	36.0	36.0	36.0	36.0	36.0
13	35.137705233390015	36.0	36.0	36.0	36.0	36.0
14	35.079492176804365	36.0	36.0	36.0	36.0	36.0
15	35.05075564080278	36.0	36.0	36.0	36.0	36.0
16	35.06475777791195	36.0	36.0	36.0	36.0	36.0
17	35.02709930256102	36.0	36.0	36.0	36.0	36.0
18	35.0183493710131	36.0	36.0	36.0	36.0	36.0
19	35.02175793377208	36.0	36.0	36.0	36.0	36.0
20	35.0136770774681	36.0	36.0	36.0	36.0	36.0
21	35.00573031704197	36.0	36.0	36.0	36.0	36.0
22	34.990658221200626	36.0	36.0	36.0	36.0	36.0
23	34.93413154314166	36.0	36.0	36.0	36.0	36.0
24	34.903771815152695	36.0	36.0	36.0	32.0	36.0
25	34.87615508504206	36.0	36.0	36.0	32.0	36.0
26	34.815669419259535	36.0	36.0	36.0	32.0	36.0
27	34.80678539183377	36.0	36.0	36.0	32.0	36.0
28	34.773479766968045	36.0	36.0	36.0	32.0	36.0
29	34.743440886184196	36.0	36.0	36.0	32.0	36.0
30	34.72605124849581	36.0	36.0	36.0	32.0	36.0
31	34.713286163411624	36.0	36.0	36.0	32.0	36.0
32	34.67580812073403	36.0	36.0	36.0	32.0	36.0
33	34.65881234580729	36.0	36.0	36.0	32.0	36.0
34	34.64927398666343	36.0	36.0	36.0	32.0	36.0
35	34.597214596933064	36.0	36.0	36.0	32.0	36.0
36	34.5687118511269	36.0	36.0	36.0	32.0	36.0
37	34.547420795672984	36.0	36.0	36.0	32.0	36.0
38	34.502577308730835	36.0	36.0	36.0	32.0	36.0
39	34.48271637528311	36.0	36.0	36.0	32.0	36.0
40	34.465776766169085	36.0	36.0	36.0	32.0	36.0
41	34.45628755211134	36.0	36.0	36.0	32.0	36.0
42	34.38395511228248	36.0	36.0	36.0	32.0	36.0
43	34.376519460752036	36.0	36.0	36.0	32.0	36.0
44	34.29218649296453	36.0	36.0	36.0	32.0	36.0
45	34.26698278658255	36.0	36.0	36.0	32.0	36.0
46	34.22381023256859	36.0	36.0	36.0	32.0	36.0
47	34.19445657469983	36.0	36.0	36.0	32.0	36.0
48	34.144346138670585	36.0	36.0	36.0	32.0	36.0
49	34.12292239148414	36.0	36.0	36.0	32.0	36.0
50	33.545293515516505	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	2.0
20	6.0
21	45.0
22	149.0
23	415.0
24	1191.0
25	2692.0
26	5574.0
27	9914.0
28	16509.0
29	25288.0
30	36415.0
31	51561.0
32	74114.0
33	118749.0
34	246207.0
35	835522.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.03470807219661	17.66220434735596	11.532541977304101	26.77054560314333
2	15.990274841734491	20.2968096959519	37.24485676594257	26.46805869637104
3	18.207858731549504	24.401903462065558	28.699015411920648	28.69122239446429
4	12.168814774978692	15.66394309279856	35.87591286997474	36.291329262248006
5	14.147606564098531	37.088251937369506	33.39773679857676	15.366404699955208
6	34.419463139079184	35.80563539681849	16.56308754705642	13.211813917045903
7	30.230687034262548	30.305036528840446	20.930892179893483	18.533384257003526
8	28.16062579948524	33.35933342413473	19.186101207986216	19.29393956839381
9	27.95225283921771	13.427590119185362	18.175837723683173	40.44431931791375
10	15.991664923171541	26.62450450804652	31.586374620702053	25.797455948079882
11	37.82510374886001	20.373179963113078	22.77455097156393	19.027165316462984
12	25.30141797913576	23.204182115223006	29.02009406389296	22.474305841748276
13	30.2372947041697	19.125746745701715	25.055762394978192	25.581196155150394
14	23.60431465773256	19.66582745581506	24.90342990576781	31.826427980684578
15	25.23022158564327	27.891004577751566	21.83875361586205	25.040020220743113
16	25.63515337970332	25.142083251131574	24.362143615889735	24.860619753275373
17	24.063329762123743	25.83192099716784	25.195843168201165	24.908906072507257
18	24.95603254539442	24.329500705234462	26.806140910689724	23.908325838681392
19	26.17990496731145	24.196336298892408	25.02380029655591	24.599958437240232
20	26.488412846407027	23.812990754990395	24.84912267891846	24.84947371968412
21	27.181094025782915	23.607614399229405	24.08060074953277	25.13069082545491
22	26.46375334187055	24.268298847475904	24.64138078227854	24.626567028375007
23	24.419611232375257	24.042806984533964	25.57192875050813	25.965653032582654
24	25.440131203891188	24.456523265381776	25.371678831296844	24.731666699430196
25	25.173169625493042	24.274849999227705	25.31169128415826	25.240289091120992
26	24.524323732278866	25.23394145004381	25.799065919252286	24.442668898425037
27	25.157115685309446	24.707621945011795	24.91025220657053	25.225010163108237
28	24.480327681243775	24.539373774407416	25.54428070835653	25.43601783599228
29	24.781930414318172	24.363554155117264	25.264126780854813	25.590388649709755
30	24.405200297970307	24.773520802806708	25.85285574768852	24.96842315153447
31	25.680724308498128	24.192226813316438	24.5610512162499	25.565997661935537
32	24.989925002703046	24.756269316262237	24.49123018876403	25.762575492270685
33	24.401987780681345	24.530120718780147	25.454924587622568	25.61296691291594
34	25.395698707309332	24.545466034222745	25.370072371580466	24.68876288688746
35	25.74789002651816	24.488681162137098	25.546177895636262	24.217250915708483
36	24.580130482526968	24.949306723810114	25.163594644767922	25.306968148895
37	25.582427272944287	24.74334724896054	24.492908085246206	25.181317392848968
38	24.78204057903447	24.953852354214515	25.00623142421219	25.257875642538824
39	25.87195770539109	24.18501785093678	24.472598724281134	25.470425719391
40	26.0711565127341	24.486616467449494	25.16932582100293	24.272901198813475
41	24.94023400886748	24.787949210316608	25.64043515960416	24.631381621211748
42	26.043662481087665	25.367978740956303	24.93718121088087	23.65117756707516
43	25.05000565174442	24.447691174643115	25.229316897171252	25.272986276441213
44	24.68060668056315	25.110208861501565	24.61011794226242	25.599066515672863
45	24.880032407924215	25.15229828770177	24.874134952044557	25.09353435232946
46	24.731349633725607	24.61705124190679	24.967669281675327	25.683929842692276
47	25.416248633567108	24.4568495059779	25.200848961159956	24.926052899295037
48	25.718285592724715	25.13492318137172	24.19756952221011	24.949221703693457
49	25.181135509018866	25.228034160191186	24.289499476251592	25.301330854538357
50	24.91295962548242	25.90373499567168	24.149658192326406	25.033647186519502
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	17.5
2	17.0
3	78.0
4	139.0
5	171.0
6	203.0
7	196.5
8	190.0
9	273.0
10	356.0
11	537.0
12	718.0
13	1394.0
14	2070.0
15	2724.5
16	3379.0
17	3599.0
18	3819.0
19	3808.0
20	3797.0
21	4128.5
22	4460.0
23	5009.5
24	5559.0
25	6124.0
26	6689.0
27	8627.5
28	10566.0
29	13147.0
30	15728.0
31	19066.5
32	22405.0
33	27228.0
34	32051.0
35	38485.5
36	44920.0
37	50736.0
38	56552.0
39	59354.0
40	62156.0
41	66469.0
42	70782.0
43	71798.0
44	72814.0
45	80098.0
46	87382.0
47	93715.5
48	100049.0
49	104917.5
50	109786.0
51	107021.0
52	104256.0
53	103299.5
54	102343.0
55	102934.0
56	103525.0
57	101106.0
58	98687.0
59	90286.0
60	81885.0
61	72552.0
62	63219.0
63	55102.0
64	46985.0
65	39979.5
66	32974.0
67	28451.5
68	23929.0
69	21471.0
70	19013.0
71	14832.0
72	10651.0
73	9210.0
74	7769.0
75	6084.5
76	4400.0
77	3826.5
78	3253.0
79	2645.5
80	2038.0
81	1634.5
82	1231.0
83	1031.0
84	831.0
85	601.5
86	372.0
87	273.0
88	174.0
89	130.0
90	86.0
91	71.5
92	57.0
93	39.0
94	21.0
95	25.0
96	29.0
97	20.0
98	11.0
99	10.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009267359097527721
2	4.914508612325307E-4
3	1.4041453178072305E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0012637307860265076
10	8.424871906843382E-4
11	7.020726589036153E-5
12	2.1062179767108454E-4
13	4.914508612325307E-4
14	0.0
15	0.005195337675886753
16	9.126944565746998E-4
17	0.0
18	3.510363294518076E-4
19	1.4041453178072305E-4
20	0.0012637307860265076
21	0.0
22	1.4041453178072305E-4
23	4.914508612325307E-4
24	4.212435953421691E-4
25	0.0019658034449301226
26	0.004633679548763861
27	0.00596761760068073
28	0.0030189124332855454
29	0.0026678761038337376
30	0.003580570560408438
31	0.006950519323145791
32	0.002527461572053015
33	0.0036507778262987994
34	0.0026678761038337376
35	0.0032997414968469916
36	0.007161141120816876
37	0.003931606889860245
38	0.008495079172733745
39	0.00414222868753133
40	0.002036010710820484
41	0.003440156028627715
42	0.0013339380519168688
43	0.001053108988355423
44	9.126944565746998E-4
45	7.722799247939768E-4
46	8.424871906843382E-4
47	0.0017551816472590382
48	0.0017551816472590382
49	4.212435953421691E-4
50	0.001053108988355423
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1424354.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.46957724801607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.64707337465522	39.691699600178126
2	13.984705027348717	14.675431214463849
3	4.632118278146752	7.2913586345150465
4	1.9350264316645518	4.061200753327044
5	1.04433037024206	2.7397786516932503
6	0.6308134167672594	1.985910798009281
7	0.38865021072565326	1.4274618577889173
8	0.2948872875664554	1.237808905154085
9	0.22850674319779743	1.0790686992518463
>10	1.0506748991323172	9.687477861580135
>50	0.07649904740951222	2.8019783433495378
>100	0.07484729380404273	8.25638207395007
>500	0.009170433126199978	3.2662866865123785
>1k	0.0026971862135882286	1.7981559202264419
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1701	0.11942255927950496	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1505	0.10566193516499409	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1452	0.10194095007280493	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1429	0.10032618295732663	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.808290635614461E-4
2	0.0	0.0	0.0	0.0	2.808290635614461E-4
3	0.0	7.020726589036153E-5	0.0	0.0	2.808290635614461E-4
4	0.0	7.020726589036153E-5	0.0	0.0	2.808290635614461E-4
5	0.0	1.4041453178072305E-4	0.0	0.0	4.2124359534216914E-4
6	0.0	1.4041453178072305E-4	0.0	0.0	5.616581271228922E-4
7	0.0	1.4041453178072305E-4	0.0	0.0	5.616581271228922E-4
8	0.0	1.4041453178072305E-4	0.0	0.0	5.616581271228922E-4
9	0.0	1.4041453178072305E-4	0.0	7.020726589036153E-5	5.616581271228922E-4
10	0.0	1.4041453178072305E-4	0.0	7.020726589036153E-5	5.616581271228922E-4
11	0.0	1.4041453178072305E-4	0.0	7.020726589036153E-5	5.616581271228922E-4
12	0.0	2.1062179767108457E-4	0.0	1.4041453178072305E-4	0.001053108988355423
13	0.0	2.1062179767108457E-4	0.0	1.4041453178072305E-4	0.0012637307860265074
14	0.0	2.1062179767108457E-4	0.0	1.4041453178072305E-4	0.0012637307860265074
15	0.0	2.1062179767108457E-4	0.0	1.4041453178072305E-4	0.001474352583697592
16	0.0	2.1062179767108457E-4	0.0	1.4041453178072305E-4	0.001614767115478315
17	0.0	2.1062179767108457E-4	0.0	2.1062179767108457E-4	0.001614767115478315
18	0.0	2.1062179767108457E-4	0.0	2.808290635614461E-4	0.0018253889131493997
19	0.0	2.1062179767108457E-4	0.0	3.510363294518076E-4	0.0018253889131493997
20	0.0	3.510363294518076E-4	0.0	3.510363294518076E-4	0.0023168397743819303
21	0.0	3.510363294518076E-4	0.0	4.2124359534216914E-4	0.0024572543061626533
22	0.0	3.510363294518076E-4	0.0	4.914508612325307E-4	0.0024572543061626533
23	0.0	3.510363294518076E-4	0.0	0.001053108988355423	0.0024572543061626533
24	0.0	3.510363294518076E-4	0.0	0.0014041453178072305	0.0024572543061626533
25	0.0	3.510363294518076E-4	0.0	0.001614767115478315	0.0024572543061626533
26	0.0	3.510363294518076E-4	0.0	0.0019658034449301226	0.0027380833697240995
27	0.0	3.510363294518076E-4	0.0	0.0024572543061626533	0.002808290635614461
28	0.0	3.510363294518076E-4	0.0	0.004282643219312053	0.002808290635614461
29	0.0	3.510363294518076E-4	0.0	0.008565286438624106	0.002808290635614461
30	0.0	3.510363294518076E-4	0.0	0.01614767115478315	0.002808290635614461
31	0.0	3.510363294518076E-4	0.0	0.03461218208394823	0.002808290635614461
32	0.0	3.510363294518076E-4	0.0	0.05384897293790729	0.002808290635614461
33	0.0	3.510363294518076E-4	0.0	0.0728049347283049	0.002808290635614461
34	0.0	3.510363294518076E-4	0.0	0.09520105254733023	0.003018912433285546
35	0.0	4.2124359534216914E-4	0.0	0.11914173021594351	0.003018912433285546
36	0.0	4.2124359534216914E-4	0.0	0.15684303199906766	0.003018912433285546
37	0.0	4.2124359534216914E-4	0.0	0.20942827415094842	0.003018912433285546
38	0.0	4.2124359534216914E-4	0.0	0.2802674054343232	0.0030891196991759073
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2865	0.0	20.964287	1
GTATAGA	180	0.0	19.556429	1
ACCGTCG	395	0.0	18.936226	8
ATACGCA	70	8.119937E-4	18.858648	25
CGTAAGG	70	8.127607E-4	18.855999	2
GTCCTAA	550	0.0	18.800838	1
CATAACG	60	0.007415327	18.332224	2
GGCGTTA	460	0.0	18.17345	42
TGTAGGA	1195	0.0	18.040747	2
GTAGGAC	1120	0.0	17.873915	3
ATACCGT	450	0.0	17.599548	6
TAGGACG	1060	0.0	17.433517	4
TACCGTC	455	0.0	17.406149	7
GTCTTAC	165	1.6589183E-9	17.334108	1
TAGGACA	530	0.0	17.018435	4
GATTTCG	440	0.0	16.999567	41
TGTACTA	130	7.320996E-7	16.922644	5
TTAGGAC	700	0.0	16.656134	3
CCGTCGT	445	0.0	16.314192	9
AACGCAG	3615	0.0	16.309404	6
>>END_MODULE