FastQCFastQC Report
Thu 2 Feb 2017
SRR4063983_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063983_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2701843
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46390.171697615294449No Hit
GCGCAAGACGGACCAGAGCGAAAGC31540.11673513227822639No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG30640.11340407270148561No Hit
TATCAACGCAGAGTACTTTTTTTTT30210.11181256645926502No Hit
GGGTAGGCACACGCTGAGCCAGTCA29570.10944381298247159No Hit
GATTAAGAGGGACGGCCGGGGGCAT29370.10870357752097365No Hit
GAATAGGACCGCGGTTCTATTTTGT28430.1052244708519333No Hit
GTATCTGATCGTCTTCGAACCTCCG28340.10489136489425922No Hit
GTCTTGCGCCGGTCCAAGAATTTCA28240.10452124716351024No Hit
ATCAGATACCGTCGTAGTTCCGACC28120.10407710588661147No Hit
GAACTACGACGGTATCTGATCGTCT27720.10259663496361558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35400.012.5291271
CGTTATT3050.012.4531392
CGCATCG4450.012.38306113
CGGTCCA4950.012.28385710
ATCGCCA4650.012.26045616
CCGTCGT5650.012.1072099
CGTCGTA5850.012.01810110
CGCCAGT4800.011.87709618
GCATCGC4800.011.87643614
CGGTTCT5150.011.80703412
GCGTTAT3300.011.7998671
TCGCGTA2900.011.7940929
CGCGTAA2950.011.594192510
CGTCTTA2050.011.58741115
CGGACAT3400.011.4540845
CGACCAT4250.011.40095610
ACGGTAT4000.011.4009569
GTCGTAG6200.011.33924111
GGTATCA13150.011.1947171
CGAACGA2650.011.115375516
Produced by FastQC (version 0.11.5)