##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063983_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2701843
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21252641252656	32.0	32.0	32.0	32.0	32.0
2	30.75610277873289	32.0	32.0	32.0	32.0	32.0
3	30.771894591950755	32.0	32.0	32.0	32.0	32.0
4	30.809392329606123	32.0	32.0	32.0	32.0	32.0
5	30.69639020476023	32.0	32.0	32.0	32.0	32.0
6	34.349777910855664	36.0	36.0	36.0	32.0	36.0
7	34.28077279101709	36.0	36.0	36.0	32.0	36.0
8	34.24273098029752	36.0	36.0	36.0	32.0	36.0
9	34.41193067102715	36.0	36.0	36.0	32.0	36.0
10	34.05589295899133	36.0	36.0	36.0	32.0	36.0
11	34.37561545952152	36.0	36.0	36.0	32.0	36.0
12	34.163812257040846	36.0	36.0	36.0	32.0	36.0
13	34.26544917672862	36.0	36.0	36.0	32.0	36.0
14	34.13825007596667	36.0	36.0	36.0	32.0	36.0
15	34.07442438365219	36.0	36.0	36.0	32.0	36.0
16	34.07462572769772	36.0	36.0	36.0	32.0	36.0
17	33.98527338561123	36.0	36.0	36.0	32.0	36.0
18	33.9894268467857	36.0	36.0	36.0	32.0	36.0
19	34.00803303522817	36.0	36.0	36.0	32.0	36.0
20	34.001395343844926	36.0	36.0	36.0	32.0	36.0
21	33.977799228156485	36.0	36.0	36.0	32.0	36.0
22	33.95975931984205	36.0	36.0	36.0	32.0	36.0
23	33.88817447942016	36.0	36.0	36.0	32.0	36.0
24	33.8778233968443	36.0	36.0	36.0	32.0	36.0
25	33.46463987729857	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	10.0
4	30.0
5	33.0
6	109.0
7	34.0
8	164.0
9	165.0
10	222.0
11	79.0
12	135.0
13	140.0
14	217.0
15	408.0
16	744.0
17	997.0
18	1361.0
19	1944.0
20	2916.0
21	4415.0
22	7046.0
23	11019.0
24	16473.0
25	23856.0
26	34076.0
27	44064.0
28	59764.0
29	79260.0
30	103034.0
31	138228.0
32	191687.0
33	267758.0
34	558947.0
35	1152508.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.41468531442639	17.095223760898133	11.384819426193726	26.105271498481752
2	17.129903461284208	19.304671034757938	36.85008849937484	26.715337004583017
3	18.96165134966078	23.082548976789464	28.26418781622554	29.69161185732422
4	12.771895330730803	15.412907808296094	35.33818528801371	36.47701157295939
5	15.130546664736947	35.99295380776066	33.26901344795179	15.607486079550606
6	34.81382522903297	35.1320227121042	16.58750597360619	13.46664608525664
7	30.982180141386078	30.058276594295396	20.275975324071467	18.683567940247055
8	28.662212385252143	32.318815574829635	18.918169425107425	20.100802614810796
9	27.338740346008823	14.181466336513775	18.337852754625235	40.14194056285218
10	16.246127866952307	26.268772786102723	30.825160116057525	26.659939230887446
11	37.587588032406735	21.031237293671442	22.02437689460848	19.356797779313336
12	25.008203199358853	23.39130260880257	28.240078572947	23.36041561889158
13	29.42289687783315	19.245634358649212	25.00792540930665	26.323543354210983
14	24.045785977553656	19.450300133919576	24.538984818497493	31.964929070029275
15	25.457887191823968	26.885885664576637	21.870855758451984	25.78537138514741
16	26.332291768128847	25.590801536089547	23.081341711779142	24.995564984002463
17	24.567869504256308	25.602449572739467	24.5763502758658	25.253330647138423
18	25.300975440818874	24.701598819173977	25.462838982815427	24.53458675719172
19	25.81470640198022	24.730369543453293	24.864263440965757	24.590660613600733
20	26.198510390522443	23.967652035285994	24.344795552803056	25.489042021388503
21	27.40882916044564	23.836999772950264	24.024973248503898	24.7291978181002
22	26.58322792370504	23.822674768546385	24.473133971779234	25.120963335969343
23	24.950400647199324	23.89525683500566	25.09338309814078	26.060959419654235
24	25.136789531544935	24.683491322217314	24.712567894722806	25.467151251514945
25	25.211794407560888	24.210752844297403	24.7823903210178	25.795062427123906
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	125.0
1	125.0
2	346.0
3	567.0
4	567.0
5	567.0
6	1567.0
7	2567.0
8	2567.0
9	2567.0
10	2948.0
11	3329.0
12	3329.0
13	3329.0
14	4687.0
15	6045.0
16	6045.0
17	6045.0
18	10631.0
19	15217.0
20	15217.0
21	15217.0
22	24787.5
23	34358.0
24	34358.0
25	34358.0
26	51300.0
27	68242.0
28	68242.0
29	68242.0
30	84789.5
31	101337.0
32	101337.0
33	101337.0
34	129478.5
35	157620.0
36	157620.0
37	157620.0
38	184051.0
39	210482.0
40	210482.0
41	210482.0
42	245728.5
43	280975.0
44	280975.0
45	280975.0
46	313046.0
47	345117.0
48	345117.0
49	345117.0
50	358471.5
51	371826.0
52	371826.0
53	371826.0
54	348179.5
55	324533.0
56	324533.0
57	324533.0
58	300416.5
59	276300.0
60	276300.0
61	276300.0
62	244027.0
63	211754.0
64	211754.0
65	211754.0
66	175629.0
67	139504.0
68	139504.0
69	139504.0
70	105651.0
71	71798.0
72	71798.0
73	71798.0
74	55678.0
75	39558.0
76	39558.0
77	39558.0
78	31341.5
79	23125.0
80	23125.0
81	23125.0
82	15880.5
83	8636.0
84	8636.0
85	8636.0
86	6553.5
87	4471.0
88	4471.0
89	4471.0
90	3246.0
91	2021.0
92	2021.0
93	2021.0
94	1402.0
95	783.0
96	783.0
97	783.0
98	1168.0
99	1553.0
100	1553.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0383441969055937
2	0.004774518726661764
3	0.0019616239729695617
4	0.004219342130538303
5	0.00895684908412517
6	0.013916426676161421
7	0.02172591079496477
8	0.0340878430019805
9	0.04722702244356907
10	0.053963165143200396
11	0.05244568244712961
12	0.06206874344660293
13	0.06169862571585396
14	0.06347519082344903
15	0.05773836599683994
16	0.06440048515032146
17	0.05984803706210909
18	0.07542999352664088
19	0.07195088685760054
20	0.0767994291304121
21	0.0738014755113454
22	0.07617022898813884
23	0.08183303026859813
24	0.0767994291304121
25	0.07605919366891414
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2701843.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.07960102454957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.14492461337122	35.64858393406193
2	14.508254311597415	13.951021577286097
3	4.995378745657163	7.205274511731338
4	2.196011309594986	4.223333904429021
5	1.1553609713829722	2.7774647271714663
6	0.6880624489110808	1.9849060814171573
7	0.4693635114183823	1.5796767255134205
8	0.3003462180368612	1.1552421065955727
9	0.21640732712542116	0.9364300152281491
>10	1.1471298417464681	9.920878572207908
>50	0.08717100525261011	2.9034783590050415
>100	0.0715695821343368	7.474682357640669
>500	0.013063317480105252	4.395921851965589
>1k	0.0069567962911744625	5.843105275746787
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4639	0.171697615294449	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3154	0.11673513227822639	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3064	0.11340407270148561	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3021	0.11181256645926502	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2957	0.10944381298247159	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2937	0.10870357752097365	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2843	0.1052244708519333	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2834	0.10489136489425922	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2824	0.10452124716351024	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2812	0.10407710588661147	No Hit
GAACTACGACGGTATCTGATCGTCT	2772	0.10259663496361558	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4804709229958958E-4
2	0.0	0.0	0.0	0.0	1.4804709229958958E-4
3	0.0	0.0	0.0	0.0	1.4804709229958958E-4
4	0.0	0.0	0.0	0.0	1.4804709229958958E-4
5	0.0	0.0	0.0	0.0	1.8505886537448698E-4
6	0.0	0.0	0.0	0.0	2.5908241152428177E-4
7	0.0	0.0	0.0	3.7011773074897394E-5	2.5908241152428177E-4
8	0.0	0.0	0.0	3.7011773074897394E-5	2.5908241152428177E-4
9	0.0	0.0	0.0	3.7011773074897394E-5	2.5908241152428177E-4
10	0.0	0.0	0.0	7.402354614979479E-5	2.5908241152428177E-4
11	0.0	0.0	0.0	7.402354614979479E-5	2.5908241152428177E-4
12	0.0	0.0	0.0	7.402354614979479E-5	6.662119153481531E-4
13	0.0	0.0	0.0	7.402354614979479E-5	8.882825537975375E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3540	0.0	12.529127	1
CGTTATT	305	0.0	12.453139	2
CGCATCG	445	0.0	12.383061	13
CGGTCCA	495	0.0	12.283857	10
ATCGCCA	465	0.0	12.260456	16
CCGTCGT	565	0.0	12.107209	9
CGTCGTA	585	0.0	12.018101	10
CGCCAGT	480	0.0	11.877096	18
GCATCGC	480	0.0	11.876436	14
CGGTTCT	515	0.0	11.807034	12
GCGTTAT	330	0.0	11.799867	1
TCGCGTA	290	0.0	11.794092	9
CGCGTAA	295	0.0	11.5941925	10
CGTCTTA	205	0.0	11.587411	15
CGGACAT	340	0.0	11.454084	5
CGACCAT	425	0.0	11.400956	10
ACGGTAT	400	0.0	11.400956	9
GTCGTAG	620	0.0	11.339241	11
GGTATCA	1315	0.0	11.194717	1
CGAACGA	265	0.0	11.1153755	16
>>END_MODULE