FastQCFastQC Report
Thu 2 Feb 2017
SRR4063983_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063983_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2701843
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA31090.11506960248985601No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT30570.11314499028996132No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA30300.1121456724169391No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC29940.1108132485862428No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT29400.10881461284019833No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG28970.10722310659797775No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC28220.10444722361736045No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT27960.10348491751741311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40400.027.1191221
TCGCGAT450.001399623524.44155717
ACCGTCG8050.018.8552638
TTCGCGA708.1333314E-418.85491416
AACGCAG57800.018.8008946
ATACCGT8650.018.3103146
TACCGTC8400.018.0696287
TCAACGC59900.018.0315914
ATCAACG60600.017.8229773
CGTCGTA8300.017.75891110
CAACGCA61400.017.698565
GGTATCA16950.017.6521171
TATCAAC62600.017.3251132
TAAACGC7500.017.01447128
CCGTCGT8650.016.7860079
ATACGAA9200.016.7383940
GTATTAC1854.2382453E-1016.6488381
CGCAATA9150.016.35233536
GTCGTAG9350.016.23487711
TACGAAT9600.016.04095841
Produced by FastQC (version 0.11.5)