FastQCFastQC Report
Thu 2 Feb 2017
SRR4063982_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063982_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933500
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26520.28409212640599896No Hit
TATCAACGCAGAGTACTTTTTTTTT19570.2096411355115158No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17360.18596679164434923No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10750.11515800749866095No Hit
GGTATCAACGCAGAGTACTTTTTTT10150.10873058382431708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATGCG400.005277799414.24967913
TAGTACA400.00527967714.2489154
GCGTTAT801.2902638E-714.2466241
GTATATA801.2902638E-714.2466241
AACCGCG954.8821676E-914.0019357
AGAACCG1205.0931703E-1113.4580295
CGTTATT852.7081842E-713.4064322
TATACCG500.001499018413.29975
GTCTAAA500.001501442313.2968491
GAACCGC1102.73576E-912.9556426
TCGCGTA604.0845346E-412.6684189
TCGTCGC604.0900803E-412.6663813
CGGACAT701.0902292E-412.2140095
CGGTCCA1251.4042598E-912.16102910
CGCGGTC1103.801688E-812.09193210
TATACTG550.003067384512.0906355
GTATTAT1103.8186045E-812.0880441
GCTATAC550.003074781512.0867483
TGCGGTA1353.6925485E-1011.96333515
TAGAACC1603.6379788E-1211.8740964
Produced by FastQC (version 0.11.5)