##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063982_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	933500
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25785216925549	32.0	32.0	32.0	32.0	32.0
2	30.896765934654525	32.0	32.0	32.0	32.0	32.0
3	30.912791644349223	32.0	32.0	32.0	32.0	32.0
4	30.928104981253348	32.0	32.0	32.0	32.0	32.0
5	30.863361542581682	32.0	32.0	32.0	32.0	32.0
6	34.51048205677557	36.0	36.0	36.0	32.0	36.0
7	34.448334226031065	36.0	36.0	36.0	32.0	36.0
8	34.40453668987681	36.0	36.0	36.0	32.0	36.0
9	34.52964649169791	36.0	36.0	36.0	32.0	36.0
10	34.25771612212105	36.0	36.0	36.0	32.0	36.0
11	34.52034172469202	36.0	36.0	36.0	32.0	36.0
12	34.34534118907338	36.0	36.0	36.0	32.0	36.0
13	34.41489341189073	36.0	36.0	36.0	32.0	36.0
14	34.32490626673808	36.0	36.0	36.0	32.0	36.0
15	34.25512587038029	36.0	36.0	36.0	32.0	36.0
16	34.2635961435458	36.0	36.0	36.0	32.0	36.0
17	34.20374076057847	36.0	36.0	36.0	32.0	36.0
18	34.18793572576325	36.0	36.0	36.0	32.0	36.0
19	34.204440278521695	36.0	36.0	36.0	32.0	36.0
20	34.183811462238886	36.0	36.0	36.0	32.0	36.0
21	34.172188537761116	36.0	36.0	36.0	32.0	36.0
22	34.14407070166042	36.0	36.0	36.0	32.0	36.0
23	34.09444349223353	36.0	36.0	36.0	32.0	36.0
24	34.085163363685055	36.0	36.0	36.0	32.0	36.0
25	33.69932297803964	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	11.0
5	15.0
6	35.0
7	19.0
8	57.0
9	64.0
10	88.0
11	30.0
12	57.0
13	62.0
14	100.0
15	173.0
16	262.0
17	339.0
18	458.0
19	642.0
20	877.0
21	1349.0
22	2120.0
23	3103.0
24	4741.0
25	6898.0
26	9934.0
27	13380.0
28	18023.0
29	24688.0
30	32598.0
31	43175.0
32	61509.0
33	86535.0
34	188108.0
35	434046.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.72435648063306	16.936141573724882	11.08817707646786	26.25132486917419
2	16.9134567176459	19.848047891053852	36.89338069873973	26.345114692560518
3	18.75196174319563	23.935677319624073	28.435608223413222	28.876752713767072
4	12.728344010455725	15.246930773680715	35.61963019304523	36.40509502281834
5	14.682098388475707	36.533026498764734	33.55992380560573	15.22495130715383
6	34.19605406245145	35.19512730818061	16.9849951519502	13.62382347741773
7	30.338026146746227	30.363313549869225	20.961328488724067	18.33733181466048
8	28.50729745920464	32.194124524585455	19.63589327566381	19.662684740546098
9	27.85092866796784	13.846257729074827	18.37432462703764	39.92848897591969
10	15.906194293750609	26.62258938993919	31.776264943879724	25.69495137243048
11	37.11540770213605	20.919655796324722	22.600692866391693	19.364243635147535
12	24.818590251416918	23.58675132633601	28.801939897066248	22.79271852518082
13	29.72886345193391	19.23458301486518	25.19234047023587	25.84421306296504
14	23.769399571405753	19.165173876548135	25.51893887011064	31.54648768193547
15	25.426277815116507	26.785264460001653	22.270315928485445	25.518141796396403
16	25.782618483539338	25.42467544997481	23.65972363667549	25.13298242981036
17	24.173450507796503	25.4462113042341	25.320253758894744	25.060084429074646
18	24.86448586107228	24.695950871836217	26.009172934910328	24.430390332181176
19	25.632545329601115	24.673368504222328	25.628900307575535	24.06518585860102
20	25.922503093057614	23.926113983193495	25.11820090186484	25.033182021884056
21	26.81000471718341	23.930164243749733	24.562588447188986	24.69724259187787
22	25.997066694112803	23.965302079044875	24.858802490736927	25.17882873610539
23	24.75701975685357	23.94345810875865	25.18942541404477	26.110096720343005
24	24.84524123498245	24.636716262374804	25.315682150047923	25.202360352594823
25	25.125569417776017	24.237872701288982	25.244143399471024	25.39241448146397
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	250.0
1	250.0
2	400.5
3	551.0
4	551.0
5	551.0
6	1444.5
7	2338.0
8	2338.0
9	2338.0
10	2280.0
11	2222.0
12	2222.0
13	2222.0
14	2327.5
15	2433.0
16	2433.0
17	2433.0
18	4164.5
19	5896.0
20	5896.0
21	5896.0
22	9243.0
23	12590.0
24	12590.0
25	12590.0
26	18376.5
27	24163.0
28	24163.0
29	24163.0
30	30592.5
31	37022.0
32	37022.0
33	37022.0
34	45963.5
35	54905.0
36	54905.0
37	54905.0
38	64201.5
39	73498.0
40	73498.0
41	73498.0
42	84893.5
43	96289.0
44	96289.0
45	96289.0
46	108657.5
47	121026.0
48	121026.0
49	121026.0
50	125951.0
51	130876.0
52	130876.0
53	130876.0
54	121468.5
55	112061.0
56	112061.0
57	112061.0
58	103198.5
59	94336.0
60	94336.0
61	94336.0
62	82508.0
63	70680.0
64	70680.0
65	70680.0
66	58110.5
67	45541.0
68	45541.0
69	45541.0
70	34202.0
71	22863.0
72	22863.0
73	22863.0
74	17286.0
75	11709.0
76	11709.0
77	11709.0
78	9382.0
79	7055.0
80	7055.0
81	7055.0
82	4814.0
83	2573.0
84	2573.0
85	2573.0
86	1960.5
87	1348.0
88	1348.0
89	1348.0
90	968.0
91	588.0
92	588.0
93	588.0
94	397.5
95	207.0
96	207.0
97	207.0
98	343.5
99	480.0
100	480.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0416711301553294
2	0.005141938939475094
3	0.0020353508302088912
4	0.004284949116229245
5	0.01049812533476165
6	0.0144617032672737
7	0.024531333690412428
8	0.03931440814140332
9	0.05324049276914836
10	0.06352437064809856
11	0.06041778253883236
12	0.07145152651312266
13	0.0695232994108195
14	0.07316550615961435
15	0.06416711301553295
16	0.07177289769683985
17	0.0692019282271023
18	0.08098553829673273
19	0.07723620782003214
20	0.08205677557579004
21	0.07927155865024102
22	0.08184252811997858
23	0.08687734333154794
24	0.08119978575254419
25	0.08023567220139262
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	933500.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.14320885641443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.63759368613918	45.722420336590474
2	13.040391805205909	15.164204485991247
3	3.7340909260400053	6.51336085804758
4	1.5819493076016322	3.679184359685676
5	0.799105054034412	2.3231266027469575
6	0.4913396061153475	1.7140836802675832
7	0.3304272188562085	1.3448469158460483
8	0.23616195874603071	1.0984971273048312
9	0.17283558033571522	0.9044293720751178
>10	0.8371422583692185	8.597192952903834
>50	0.06791210956018733	2.7825938298741955
>100	0.06549966962885226	7.515811077647749
>500	0.004625682806161738	1.7328042129902914
>1k	9.251365612323477E-4	0.9074441880283795
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2652	0.28409212640599896	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1957	0.2096411355115158	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1736	0.18596679164434923	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1075	0.11515800749866095	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1015	0.10873058382431708	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	3.2137118371719335E-4
13	0.0	0.0	0.0	0.0	4.2849491162292447E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATGCG	40	0.0052777994	14.249679	13
TAGTACA	40	0.005279677	14.248915	4
GCGTTAT	80	1.2902638E-7	14.246624	1
GTATATA	80	1.2902638E-7	14.246624	1
AACCGCG	95	4.8821676E-9	14.001935	7
AGAACCG	120	5.0931703E-11	13.458029	5
CGTTATT	85	2.7081842E-7	13.406432	2
TATACCG	50	0.0014990184	13.2997	5
GTCTAAA	50	0.0015014423	13.296849	1
GAACCGC	110	2.73576E-9	12.955642	6
TCGCGTA	60	4.0845346E-4	12.668418	9
TCGTCGC	60	4.0900803E-4	12.66638	13
CGGACAT	70	1.0902292E-4	12.214009	5
CGGTCCA	125	1.4042598E-9	12.161029	10
CGCGGTC	110	3.801688E-8	12.091932	10
TATACTG	55	0.0030673845	12.090635	5
GTATTAT	110	3.8186045E-8	12.088044	1
GCTATAC	55	0.0030747815	12.086748	3
TGCGGTA	135	3.6925485E-10	11.963335	15
TAGAACC	160	3.6379788E-12	11.874096	4
>>END_MODULE