FastQCFastQC Report
Thu 2 Feb 2017
SRR4063982_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063982_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933500
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9520.10198178896625604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC1102.0190782E-1024.0016961
CGAGCCG2750.023.20039615
CGTCGTA2500.021.12262310
GTATTAG1700.020.7073441
TAGTACA550.00448241720.0003414
CCGTCGT2600.019.4629149
ACCGTCG2600.019.461878
ATACCGC3300.019.33366227
ATACCGT2650.019.0946666
CTACGCT708.117576E-418.85847333
GTGTATA951.5955033E-518.5276241
GTATCAA20300.018.4249481
GCTTATA600.007407850618.334631
TACGCCA600.007410140218.3336455
GGATACC3850.018.28700625
TGGATAC3650.018.082524
GTATAGA1102.7931565E-618.0012721
TACCGCA3600.017.72252528
AGGCCCG3900.017.48935110
GCATTCG3400.017.47088422
Produced by FastQC (version 0.11.5)