##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063982_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	933500
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.315637921799677	32.0	32.0	32.0	32.0	32.0
2	31.435231922870916	32.0	32.0	32.0	32.0	32.0
3	31.49497803963578	32.0	32.0	32.0	32.0	32.0
4	31.579302624531334	32.0	32.0	32.0	32.0	32.0
5	31.53149758971612	32.0	32.0	32.0	32.0	32.0
6	35.12479914301018	36.0	36.0	36.0	36.0	36.0
7	35.13286770219604	36.0	36.0	36.0	36.0	36.0
8	35.08732726298875	36.0	36.0	36.0	36.0	36.0
9	35.191957150508834	36.0	36.0	36.0	36.0	36.0
10	35.04956829137654	36.0	36.0	36.0	36.0	36.0
11	35.184762720942686	36.0	36.0	36.0	36.0	36.0
12	35.093276914836636	36.0	36.0	36.0	36.0	36.0
13	35.13240064274237	36.0	36.0	36.0	36.0	36.0
14	35.07888912694162	36.0	36.0	36.0	36.0	36.0
15	35.05602249598286	36.0	36.0	36.0	36.0	36.0
16	35.06788002142475	36.0	36.0	36.0	36.0	36.0
17	35.02905088377076	36.0	36.0	36.0	36.0	36.0
18	35.018230316015	36.0	36.0	36.0	36.0	36.0
19	35.02064595607927	36.0	36.0	36.0	36.0	36.0
20	35.01204177825388	36.0	36.0	36.0	36.0	36.0
21	35.00230208891269	36.0	36.0	36.0	36.0	36.0
22	34.99728334226031	36.0	36.0	36.0	36.0	36.0
23	34.94499303695768	36.0	36.0	36.0	36.0	36.0
24	34.91670808784146	36.0	36.0	36.0	32.0	36.0
25	34.89110016068559	36.0	36.0	36.0	32.0	36.0
26	34.82833208355651	36.0	36.0	36.0	32.0	36.0
27	34.82966791644349	36.0	36.0	36.0	32.0	36.0
28	34.80893519014462	36.0	36.0	36.0	32.0	36.0
29	34.782005356186396	36.0	36.0	36.0	32.0	36.0
30	34.75881521156936	36.0	36.0	36.0	32.0	36.0
31	34.75008248527049	36.0	36.0	36.0	32.0	36.0
32	34.70937653990359	36.0	36.0	36.0	32.0	36.0
33	34.67643385109802	36.0	36.0	36.0	32.0	36.0
34	34.66845956079271	36.0	36.0	36.0	32.0	36.0
35	34.62522335297268	36.0	36.0	36.0	32.0	36.0
36	34.598757364756295	36.0	36.0	36.0	32.0	36.0
37	34.59025602570969	36.0	36.0	36.0	32.0	36.0
38	34.55793465452598	36.0	36.0	36.0	32.0	36.0
39	34.5540203535083	36.0	36.0	36.0	32.0	36.0
40	34.53135511515801	36.0	36.0	36.0	32.0	36.0
41	34.50915800749866	36.0	36.0	36.0	32.0	36.0
42	34.44576647027316	36.0	36.0	36.0	32.0	36.0
43	34.417572576325654	36.0	36.0	36.0	32.0	36.0
44	34.37165291912159	36.0	36.0	36.0	32.0	36.0
45	34.330479914301016	36.0	36.0	36.0	32.0	36.0
46	34.32240278521692	36.0	36.0	36.0	32.0	36.0
47	34.26546866630959	36.0	36.0	36.0	32.0	36.0
48	34.22274450990894	36.0	36.0	36.0	32.0	36.0
49	34.188654525977505	36.0	36.0	36.0	32.0	36.0
50	33.61982860203535	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	0.0
20	8.0
21	28.0
22	116.0
23	305.0
24	768.0
25	1792.0
26	3607.0
27	6584.0
28	10761.0
29	16462.0
30	23591.0
31	33036.0
32	47210.0
33	74465.0
34	155133.0
35	559632.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.96072020107006	17.686194432004935	11.595490065416335	26.75759530150867
2	16.15279304294171	20.603364977750356	36.44721252750408	26.79662945180386
3	17.936231114005842	24.386714029840515	28.848757787928392	28.82829706822525
4	12.400428494911623	15.831173004820567	35.675950723085165	36.09244777718265
5	14.32394215318693	36.86106052490627	33.37354043920728	15.441456882699518
6	34.248529989919625	35.521163967318586	16.694322531298976	13.535983511462813
7	30.149791269192576	30.50919176131951	20.991559712436757	18.34945725705116
8	28.423888591322978	32.49019817889662	19.483985002678093	19.601928227102302
9	27.85850870662089	13.666100687209756	18.26381784388608	40.21157276228327
10	15.760600587475334	26.84346631872358	31.834649903694324	25.56128319010676
11	37.41992501339047	20.908730583824315	22.456025709694696	19.21531869309052
12	24.734118907337976	23.590358864488483	28.847777182645956	22.827745045527585
13	29.751096685038487	19.341292668948416	25.256053862098888	25.651556783914213
14	23.846649912479727	19.139408975702143	25.34606394443266	31.667877167385466
15	25.26680479166622	26.916912884834176	22.32739910054486	25.488883222954744
16	25.820122143913853	25.497140298385943	23.568783349348518	25.11395420835168
17	24.297696839850026	25.429566148901984	25.265559721478308	25.007177289769682
18	24.99105514955576	24.69142944066297	26.025229834450634	24.292285575330638
19	25.70133904659882	24.622388859132297	25.63867166577397	24.037600428494912
20	25.970815023985157	23.912097988846206	25.107820725146603	25.009266262022038
21	26.900267809319768	23.89994643813605	24.607177289769684	24.592608462774503
22	25.94836636314944	23.94654525977504	25.04349223352973	25.061596143545795
23	24.785937488283206	23.85780657297515	25.296066691733916	26.06018924700773
24	24.908488869225586	24.55508504080909	25.383371916018653	25.15305417394667
25	25.072765117307977	24.047143868715338	25.422958961223717	25.457132052752968
26	24.960923899360964	24.791763754305336	25.531397472775286	24.715914873558418
27	25.420298829173287	24.513441099242268	24.97870785896943	25.087552212615016
28	24.958192681121304	24.200143767897337	25.687530866710446	25.154132684270913
29	24.80197627357591	24.589436877727714	25.570824682851374	25.037762165845006
30	24.731377468488407	24.681991631189568	25.695740391187254	24.89089050913477
31	25.210006610079244	24.573424910249695	24.74987224441652	25.466696235254542
32	24.96057694779874	24.653762758928035	24.73957217418133	25.646088119091896
33	24.959317628884644	24.226124349323104	25.163399042906377	25.651158978885874
34	25.457219744008363	24.248287032870973	25.34012949560135	24.954363727519315
35	25.821743103442735	24.244729092325112	25.28953702999444	24.64399077423771
36	24.90189335381708	24.83439983801556	24.93917548635506	25.324531321812298
37	25.688434092981048	24.592724479596853	24.866651882942904	24.852189544479188
38	24.968395385512014	24.56439199149782	25.231195790051082	25.23601683293908
39	25.296156870475155	24.61931870528722	24.75247949023952	25.332044933998098
40	25.52323308069311	24.656471785532553	25.187498326146923	24.632796807627415
41	24.696665516820254	24.983770025003803	25.390858506756597	24.928705951419346
42	25.853921907857313	24.968531966701196	24.91593348380856	24.261612641632933
43	25.092341840495003	24.268764033388752	25.194110690228477	25.444783435887768
44	24.968799712903795	24.853961231299916	24.821073718377907	25.356165337418386
45	25.029004091103374	24.970299596460162	24.863817356176664	25.136878956259796
46	24.860630238417635	24.843383096515435	24.867379120031536	25.42860754503539
47	25.029620408836628	24.721418180182074	25.0276921362903	25.221269274690993
48	25.622616445987056	25.15490422933539	24.410592621159534	24.81188670351802
49	24.91226529090644	25.25677667606503	24.555648872625056	25.275309160403474
50	24.982993798494455	25.33233063698	24.60976793364207	25.074907630883477
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	135.0
1	89.0
2	43.0
3	148.5
4	254.0
5	271.0
6	288.0
7	249.0
8	210.0
9	317.0
10	424.0
11	603.5
12	783.0
13	1366.5
14	1950.0
15	2512.0
16	3074.0
17	3163.5
18	3253.0
19	3288.5
20	3324.0
21	3437.0
22	3550.0
23	4042.5
24	4535.0
25	4987.5
26	5440.0
27	6527.0
28	7614.0
29	8839.0
30	10064.0
31	11422.0
32	12780.0
33	14968.0
34	17156.0
35	19773.0
36	22390.0
37	26562.0
38	30734.0
39	34300.5
40	37867.0
41	41840.5
42	45814.0
43	48174.5
44	50535.0
45	55476.5
46	60418.0
47	64409.0
48	68400.0
49	70921.0
50	73442.0
51	72041.5
52	70641.0
53	70576.0
54	70511.0
55	70174.5
56	69838.0
57	67410.0
58	64982.0
59	59694.0
60	54406.0
61	48057.0
62	41708.0
63	36063.5
64	30419.0
65	25905.5
66	21392.0
67	18178.5
68	14965.0
69	13166.5
70	11368.0
71	9039.0
72	6710.0
73	5690.0
74	4670.0
75	3670.0
76	2670.0
77	2292.0
78	1914.0
79	1571.0
80	1228.0
81	997.0
82	766.0
83	600.5
84	435.0
85	317.5
86	200.0
87	140.5
88	81.0
89	67.0
90	53.0
91	37.5
92	22.0
93	18.0
94	14.0
95	14.0
96	14.0
97	11.5
98	9.0
99	8.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010069630423138725
2	2.1424745581146223E-4
3	4.2849491162292447E-4
4	0.0
5	0.0
6	3.2137118371719335E-4
7	1.0712372790573112E-4
8	0.0
9	0.0016068559185859668
10	0.0014997321906802356
11	0.0
12	0.0
13	5.356186395286556E-4
14	2.1424745581146223E-4
15	0.004927691483663631
16	0.0011783610069630423
17	0.0
18	2.1424745581146223E-4
19	0.0
20	6.427423674343867E-4
21	0.0
22	0.0
23	0.0011783610069630423
24	7.498660953401178E-4
25	0.0018211033743974292
26	0.006963042313872523
27	0.0061060524906266745
28	0.005463310123192287
29	0.002785216925549009
30	0.0036422067487948584
31	0.008248527048741296
32	0.0038564542046063206
33	0.005249062667380825
34	0.0034279592929833957
35	0.00289234065345474
36	0.008248527048741296
37	0.004392072844134976
38	0.009855382967327263
39	0.004499196572040707
40	0.002678093197643278
41	0.004499196572040707
42	0.0013926084627745045
43	0.0012854847348687734
44	0.0016068559185859668
45	0.0011783610069630423
46	0.0012854847348687734
47	0.0023567220139260846
48	0.0021424745581146223
49	4.2849491162292447E-4
50	0.0018211033743974292
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	933500.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.34254140365667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.63099721124517	48.847677434452756
2	12.675087913559521	15.550442102650264
3	3.560586790806212	6.552463279090293
4	1.4515216339254349	3.5616010370949747
5	0.7445026096155496	2.283484107773614
6	0.44007042051736334	1.6197022794666578
7	0.31128761800466376	1.3366621517127726
8	0.2065663090733268	1.013704189354487
9	0.14541389736412547	0.8028052217753353
>10	0.7175049545831782	7.899029884830071
>50	0.05876189226555238	2.572906208520798
>100	0.05382871114701876	6.44697337559153
>500	0.003870037893005278	1.5125487276864558
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	952	0.10198178896625604	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.0712372790573112E-4
7	0.0	0.0	0.0	0.0	1.0712372790573112E-4
8	0.0	0.0	0.0	0.0	1.0712372790573112E-4
9	0.0	0.0	0.0	0.0	1.0712372790573112E-4
10	0.0	0.0	0.0	0.0	2.1424745581146223E-4
11	0.0	0.0	0.0	0.0	2.1424745581146223E-4
12	0.0	0.0	0.0	0.0	3.2137118371719335E-4
13	0.0	0.0	0.0	0.0	5.356186395286556E-4
14	0.0	0.0	0.0	0.0	6.427423674343867E-4
15	0.0	0.0	0.0	1.0712372790573112E-4	9.6411355115158E-4
16	0.0	0.0	0.0	1.0712372790573112E-4	0.0011783610069630423
17	0.0	0.0	0.0	1.0712372790573112E-4	0.0011783610069630423
18	0.0	0.0	0.0	2.1424745581146223E-4	0.0011783610069630423
19	0.0	0.0	0.0	2.1424745581146223E-4	0.0011783610069630423
20	0.0	0.0	0.0	3.2137118371719335E-4	0.0012854847348687734
21	0.0	0.0	0.0	5.356186395286556E-4	0.0013926084627745045
22	0.0	0.0	0.0	8.569898232458489E-4	0.0013926084627745045
23	0.0	0.0	0.0	0.0017139796464916979	0.0013926084627745045
24	0.0	0.0	0.0	0.002570969469737547	0.0013926084627745045
25	0.0	0.0	0.0	0.002678093197643278	0.0013926084627745045
26	0.0	0.0	0.0	0.0033208355650776646	0.0013926084627745045
27	0.0	0.0	0.0	0.003963577932512051	0.0013926084627745045
28	0.0	0.0	0.0	0.006963042313872523	0.0013926084627745045
29	0.0	0.0	0.0	0.011783610069630423	0.0013926084627745045
30	0.0	0.0	0.0	0.02174611676486342	0.0013926084627745045
31	0.0	0.0	0.0	0.045313336904124264	0.0013926084627745045
32	0.0	0.0	0.0	0.07595072308516336	0.0014997321906802356
33	0.0	0.0	0.0	0.10562399571505088	0.0014997321906802356
34	0.0	0.0	0.0	0.13583288698446705	0.0014997321906802356
35	0.0	0.0	1.0712372790573112E-4	0.167327262988752	0.0014997321906802356
36	0.0	0.0	2.1424745581146223E-4	0.21253347616497054	0.0014997321906802356
37	0.0	0.0	2.1424745581146223E-4	0.2855918585966792	0.0014997321906802356
38	0.0	0.0	2.1424745581146223E-4	0.38746652383502944	0.0016068559185859668
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAC	110	2.0190782E-10	24.001696	1
CGAGCCG	275	0.0	23.200396	15
CGTCGTA	250	0.0	21.122623	10
GTATTAG	170	0.0	20.707344	1
TAGTACA	55	0.004482417	20.000341	4
CCGTCGT	260	0.0	19.462914	9
ACCGTCG	260	0.0	19.46187	8
ATACCGC	330	0.0	19.333662	27
ATACCGT	265	0.0	19.094666	6
CTACGCT	70	8.117576E-4	18.858473	33
GTGTATA	95	1.5955033E-5	18.527624	1
GTATCAA	2030	0.0	18.424948	1
GCTTATA	60	0.0074078506	18.33463	1
TACGCCA	60	0.0074101402	18.333645	5
GGATACC	385	0.0	18.287006	25
TGGATAC	365	0.0	18.0825	24
GTATAGA	110	2.7931565E-6	18.001272	1
TACCGCA	360	0.0	17.722525	28
AGGCCCG	390	0.0	17.489351	10
GCATTCG	340	0.0	17.470884	22
>>END_MODULE