FastQCFastQC Report
Thu 2 Feb 2017
SRR4063981_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063981_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286285
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43730.33997131273395864No Hit
TATCAACGCAGAGTACTTTTTTTTT31180.24240351088600115No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28450.22117959861150524No Hit
GTACATGGAAGCAGTGGTATCAACG25090.19505786042751022No Hit
TCCATGTACTCTGCGTTGATACCAC20390.15851852427727914No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18710.14545765518528164No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16090.12508891886323792No Hit
GAATAGGACCGCGGTTCTATTTTGT14980.116459416070311No Hit
GTACATGGGGTGGTATCAACGCAAA14910.11591521319147778No Hit
GGTATCAACGCAGAGTACTTTTTTT14770.11482680743381132No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14550.1131164555289069No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14440.11226127957645468No Hit
GATTAAGAGGGACGGCCGGGGGCAT13820.10744119693536036No Hit
GTATCTGATCGTCTTCGAACCTCCG13410.10425372293076574No Hit
GCGCAAGACGGACCAGAGCGAAAGC13200.10262111429426606No Hit
CTATTGGAGCTGGAATTACCGCGGC13020.10122173546298059No Hit
GAACTACGACGGTATCTGATCGTCT12880.10013332970531415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG753.2887328E-916.4636331
CGTTATT1150.015.6848262
GCGTTAT1300.015.3433261
CGCATCG2150.014.58551813
CCGCATC2450.013.18688812
ATCGCCA2600.013.15602816
CGCCAGT2550.012.66876918
TCGCGTA1155.313268E-912.3923959
TCACTAC1850.012.3249317
GCATCGC2550.012.29711414
GTTATTC1700.012.2880273
CGGTCCA2100.012.21583710
GTTTTAC701.0924954E-412.2120341
GTTTAAA1103.820969E-812.0886811
ATACTAG550.00307218612.0886811
TACCGGC550.003083842812.082572
AACCGCG1501.4551915E-1112.0329887
CATCGCC2800.011.87789515
ATCCCCG2100.011.76477112
GTTTAGG658.0341764E-411.6901521
Produced by FastQC (version 0.11.5)