##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063981_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1286285
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25527079923967	32.0	32.0	32.0	32.0	32.0
2	30.907784044749025	32.0	32.0	32.0	32.0	32.0
3	30.921813594965347	32.0	32.0	32.0	32.0	32.0
4	30.939654120198867	32.0	32.0	32.0	32.0	32.0
5	30.87488542585819	32.0	32.0	32.0	32.0	32.0
6	34.52957081828677	36.0	36.0	36.0	32.0	36.0
7	34.45695782816406	36.0	36.0	36.0	32.0	36.0
8	34.40979409695363	36.0	36.0	36.0	32.0	36.0
9	34.52838290114555	36.0	36.0	36.0	32.0	36.0
10	34.2676965058288	36.0	36.0	36.0	32.0	36.0
11	34.51223717916325	36.0	36.0	36.0	32.0	36.0
12	34.34777518201643	36.0	36.0	36.0	32.0	36.0
13	34.41670003148602	36.0	36.0	36.0	32.0	36.0
14	34.325285609332305	36.0	36.0	36.0	32.0	36.0
15	34.265444283343115	36.0	36.0	36.0	32.0	36.0
16	34.266810232568986	36.0	36.0	36.0	32.0	36.0
17	34.18804308531935	36.0	36.0	36.0	32.0	36.0
18	34.17912748729869	36.0	36.0	36.0	32.0	36.0
19	34.196660926622016	36.0	36.0	36.0	32.0	36.0
20	34.1651842321103	36.0	36.0	36.0	32.0	36.0
21	34.13365156244534	36.0	36.0	36.0	32.0	36.0
22	34.120303820692925	36.0	36.0	36.0	32.0	36.0
23	34.06809299649767	36.0	36.0	36.0	32.0	36.0
24	34.05281722168882	36.0	36.0	36.0	32.0	36.0
25	33.65279078897756	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	13.0
5	18.0
6	51.0
7	20.0
8	68.0
9	94.0
10	121.0
11	29.0
12	71.0
13	75.0
14	148.0
15	274.0
16	425.0
17	550.0
18	740.0
19	952.0
20	1423.0
21	2009.0
22	3047.0
23	4601.0
24	6662.0
25	9623.0
26	13604.0
27	17883.0
28	24352.0
29	33204.0
30	43825.0
31	59850.0
32	84871.0
33	120265.0
34	261880.0
35	595534.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.29787048710245	16.649102845183535	11.088150681255915	25.964875986458097
2	16.551405640287637	19.818365726929716	37.42107632418009	26.209152308602558
3	19.046863812031635	24.1388398448524	28.029424866998013	28.784871476117953
4	12.571944809205826	15.048719940228372	35.43140451661407	36.94793073395173
5	14.638796277866243	36.6273916451035	33.2093451433121	15.524466933718154
6	34.36722703907678	35.02494734116626	16.952451153051566	13.65537446670539
7	30.464832180007477	30.279216573055333	20.69625681672733	18.559694430209852
8	28.62820216688482	32.387702994768276	19.397048861844958	19.587045976501955
9	27.85242651388285	14.063614464578645	18.336391446823438	39.74756757471506
10	16.179041084701463	26.27048303598169	31.10644372099898	26.44403215831787
11	37.3125133210637	20.791905149359266	22.529127429489762	19.366454100087278
12	25.375211497695403	23.27194227814621	28.576362823080963	22.77648340107742
13	30.012338302884334	19.227447978416528	25.15761287291081	25.60260084578833
14	23.93932840209987	19.57123474808077	24.958534695490627	31.53090215432873
15	25.356280493876334	27.228542268246542	22.449482848750375	24.965694389126757
16	25.73359462337903	25.34298208285716	24.121899741484672	24.801523552279146
17	23.911235648256554	25.690350074019562	25.452771785594543	24.945642492129345
18	25.008441753457227	24.25802044382616	26.758957984187077	23.97457981852953
19	25.685905635069574	24.75212167044786	25.375474205404462	24.186498489078105
20	26.002715456706362	23.75153182102884	25.384851797531187	24.86090092473361
21	26.980109624638704	23.85833602402561	24.212029145060082	24.949525206275602
22	26.095176887902653	24.21678600800312	24.716766945549075	24.971270158545153
23	24.121059708181864	24.210152009362083	25.54357975200438	26.125208530451673
24	24.77543192154025	24.952363168111916	25.39920404280896	24.873000867538874
25	24.958003789190304	24.27742786338685	25.356996969425825	25.40757137799702
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	256.0
1	256.0
2	499.0
3	742.0
4	742.0
5	742.0
6	2174.5
7	3607.0
8	3607.0
9	3607.0
10	3429.5
11	3252.0
12	3252.0
13	3252.0
14	3031.5
15	2811.0
16	2811.0
17	2811.0
18	4936.5
19	7062.0
20	7062.0
21	7062.0
22	11635.5
23	16209.0
24	16209.0
25	16209.0
26	25267.5
27	34326.0
28	34326.0
29	34326.0
30	43513.0
31	52700.0
32	52700.0
33	52700.0
34	64995.0
35	77290.0
36	77290.0
37	77290.0
38	88583.5
39	99877.0
40	99877.0
41	99877.0
42	116131.5
43	132386.0
44	132386.0
45	132386.0
46	154541.5
47	176697.0
48	176697.0
49	176697.0
50	176931.0
51	177165.0
52	177165.0
53	177165.0
54	162298.5
55	147432.0
56	147432.0
57	147432.0
58	136184.5
59	124937.0
60	124937.0
61	124937.0
62	110875.5
63	96814.0
64	96814.0
65	96814.0
66	80088.5
67	63363.0
68	63363.0
69	63363.0
70	47804.5
71	32246.0
72	32246.0
73	32246.0
74	24939.5
75	17633.0
76	17633.0
77	17633.0
78	14369.5
79	11106.0
80	11106.0
81	11106.0
82	7583.5
83	4061.0
84	4061.0
85	4061.0
86	3116.0
87	2171.0
88	2171.0
89	2171.0
90	1584.0
91	997.0
92	997.0
93	997.0
94	693.5
95	390.0
96	390.0
97	390.0
98	572.5
99	755.0
100	755.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03871614766556401
2	0.005753001861951278
3	0.002021324978523422
4	0.004353623030665833
5	0.010961800845069328
6	0.013682815239235473
7	0.02285652091099562
8	0.03700579576065957
9	0.0492892321686096
10	0.059862316671655195
11	0.056519356130251074
12	0.06297204740784507
13	0.06662598102286818
14	0.06786987331734413
15	0.06071749262410741
16	0.06748115697532041
17	0.06328302048146406
18	0.07828747128358024
19	0.07595517323143783
20	0.08163043182498436
21	0.07696583572069954
22	0.08054202606731789
23	0.08559533851362644
24	0.08085299914093688
25	0.08163043182498436
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1286285.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.47108125895856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.953191072197	36.815332963376676
2	13.912245543675262	13.486831684841356
3	4.264416171739987	6.201025883472779
4	1.838977917274403	3.5654899224655185
5	0.9763611432654726	2.3662640156655206
6	0.6212000008075376	1.8066141430324372
7	0.4211351932403417	1.4289014720791873
8	0.30298838146028767	1.174893956662555
9	0.22856932582003983	0.9971102128615673
>10	1.2686552465336762	11.273353516982935
>50	0.09592675298158489	3.249977268469275
>100	0.09957611376696401	10.130857331856483
>500	0.012406726608585467	4.113040983963422
>1k	0.004350410628984514	3.3903066442703205
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4373	0.33997131273395864	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3118	0.24240351088600115	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2845	0.22117959861150524	No Hit
GTACATGGAAGCAGTGGTATCAACG	2509	0.19505786042751022	No Hit
TCCATGTACTCTGCGTTGATACCAC	2039	0.15851852427727914	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1871	0.14545765518528164	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1609	0.12508891886323792	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1498	0.116459416070311	No Hit
GTACATGGGGTGGTATCAACGCAAA	1491	0.11591521319147778	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1477	0.11482680743381132	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1455	0.1131164555289069	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1444	0.11226127957645468	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1382	0.10744119693536036	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1341	0.10425372293076574	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1320	0.10262111429426606	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1302	0.10122173546298059	No Hit
GAACTACGACGGTATCTGATCGTCT	1288	0.10013332970531415	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3322980521424101E-4
2	0.0	0.0	0.0	0.0	2.3322980521424101E-4
3	0.0	0.0	0.0	0.0	2.3322980521424101E-4
4	0.0	0.0	0.0	0.0	2.3322980521424101E-4
5	0.0	0.0	0.0	0.0	2.3322980521424101E-4
6	0.0	0.0	0.0	0.0	3.10973073618988E-4
7	0.0	0.0	0.0	0.0	3.10973073618988E-4
8	0.0	0.0	0.0	0.0	3.10973073618988E-4
9	0.0	0.0	0.0	0.0	3.10973073618988E-4
10	0.0	0.0	0.0	7.7743268404747E-5	3.10973073618988E-4
11	0.0	0.0	0.0	7.7743268404747E-5	3.88716342023735E-4
12	0.0	0.0	0.0	7.7743268404747E-5	5.44202878833229E-4
13	0.0	0.0	0.0	7.7743268404747E-5	6.21946147237976E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGG	75	3.2887328E-9	16.463633	1
CGTTATT	115	0.0	15.684826	2
GCGTTAT	130	0.0	15.343326	1
CGCATCG	215	0.0	14.585518	13
CCGCATC	245	0.0	13.186888	12
ATCGCCA	260	0.0	13.156028	16
CGCCAGT	255	0.0	12.668769	18
TCGCGTA	115	5.313268E-9	12.392395	9
TCACTAC	185	0.0	12.32493	17
GCATCGC	255	0.0	12.297114	14
GTTATTC	170	0.0	12.288027	3
CGGTCCA	210	0.0	12.215837	10
GTTTTAC	70	1.0924954E-4	12.212034	1
GTTTAAA	110	3.820969E-8	12.088681	1
ATACTAG	55	0.003072186	12.088681	1
TACCGGC	55	0.0030838428	12.08257	2
AACCGCG	150	1.4551915E-11	12.032988	7
CATCGCC	280	0.0	11.877895	15
ATCCCCG	210	0.0	11.764771	12
GTTTAGG	65	8.0341764E-4	11.690152	1
>>END_MODULE