FastQCFastQC Report
Thu 2 Feb 2017
SRR4063981_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063981_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286285
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14640.11381614494454961No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC14480.11257225265007367No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14190.110317697866336No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14020.10899606230345531No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA13060.10153270853659958No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12900.10028881624212363No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12880.10013332970531415No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT551.594727E-423.9977118
TACCGTA651.814848E-523.6900467
CCCTATA1057.7548975E-820.9503822
TGTAGGA6900.020.722662
AACGTAT550.004480188720.00275831
AGTATCG550.00448624119.9980938
TTAGGAC4650.019.8698443
TAATACT2000.019.798884
TAGGACG5600.019.6417474
GTAGGAC7000.019.1703433
CTATACT1152.0228435E-719.1293544
GTATCGA600.0074131118.3330089
TATGGCG7300.018.07976316
GGACGTG6350.018.014736
CTGTAGG8300.017.4936661
GATTTCG2400.017.41703641
CGGTCCG3050.017.31116939
TGGCGAG7500.017.30434218
CATGGGT9500.017.1358724
AGGACGT6450.017.0532995
Produced by FastQC (version 0.11.5)