##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063981_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1286285
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.265772359935784	32.0	32.0	32.0	32.0	32.0
2	31.418949921673658	32.0	32.0	32.0	32.0	32.0
3	31.492083014262004	32.0	32.0	32.0	32.0	32.0
4	31.573306071360545	32.0	32.0	32.0	32.0	32.0
5	31.520642003910485	32.0	32.0	32.0	32.0	32.0
6	35.10883357887249	36.0	36.0	36.0	36.0	36.0
7	35.12616566313064	36.0	36.0	36.0	36.0	36.0
8	35.07980346501747	36.0	36.0	36.0	36.0	36.0
9	35.18050509801483	36.0	36.0	36.0	36.0	36.0
10	35.03935208760112	36.0	36.0	36.0	36.0	36.0
11	35.1752721986185	36.0	36.0	36.0	36.0	36.0
12	35.080025033332426	36.0	36.0	36.0	36.0	36.0
13	35.11897985283199	36.0	36.0	36.0	36.0	36.0
14	35.06568528747517	36.0	36.0	36.0	36.0	36.0
15	35.03577123265839	36.0	36.0	36.0	36.0	36.0
16	35.04350513299929	36.0	36.0	36.0	36.0	36.0
17	35.00561306397882	36.0	36.0	36.0	36.0	36.0
18	35.00679165192784	36.0	36.0	36.0	36.0	36.0
19	35.00720524611575	36.0	36.0	36.0	36.0	36.0
20	34.99895124330922	36.0	36.0	36.0	36.0	36.0
21	34.98396700575689	36.0	36.0	36.0	36.0	36.0
22	34.96869278581341	36.0	36.0	36.0	36.0	36.0
23	34.90992042976479	36.0	36.0	36.0	32.0	36.0
24	34.880450289010604	36.0	36.0	36.0	32.0	36.0
25	34.84663896414869	36.0	36.0	36.0	32.0	36.0
26	34.779153142577265	36.0	36.0	36.0	32.0	36.0
27	34.77779574511092	36.0	36.0	36.0	32.0	36.0
28	34.742029177048636	36.0	36.0	36.0	32.0	36.0
29	34.707802703133446	36.0	36.0	36.0	32.0	36.0
30	34.68009266997594	36.0	36.0	36.0	32.0	36.0
31	34.67108222516783	36.0	36.0	36.0	32.0	36.0
32	34.62745270293908	36.0	36.0	36.0	32.0	36.0
33	34.592315855350876	36.0	36.0	36.0	32.0	36.0
34	34.58038692824685	36.0	36.0	36.0	32.0	36.0
35	34.52170941898569	36.0	36.0	36.0	32.0	36.0
36	34.479100665871094	36.0	36.0	36.0	32.0	36.0
37	34.45940440882075	36.0	36.0	36.0	32.0	36.0
38	34.4068616208694	36.0	36.0	36.0	32.0	36.0
39	34.37601697912982	36.0	36.0	36.0	32.0	36.0
40	34.34140101143992	36.0	36.0	36.0	32.0	36.0
41	34.330683324457645	36.0	36.0	36.0	32.0	36.0
42	34.236678496600675	36.0	36.0	36.0	32.0	36.0
43	34.23267705057588	36.0	36.0	36.0	32.0	36.0
44	34.161275300574914	36.0	36.0	36.0	32.0	36.0
45	34.09865387530757	36.0	36.0	36.0	32.0	36.0
46	34.07835277562904	36.0	36.0	36.0	32.0	36.0
47	34.016178374155025	36.0	36.0	36.0	32.0	36.0
48	33.983254877418304	36.0	36.0	36.0	32.0	36.0
49	33.951364588718675	36.0	36.0	36.0	32.0	36.0
50	33.356942668226715	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	4.0
20	15.0
21	58.0
22	152.0
23	466.0
24	1247.0
25	2799.0
26	5783.0
27	10343.0
28	16932.0
29	25087.0
30	35212.0
31	48242.0
32	69143.0
33	107795.0
34	219751.0
35	743252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.18050631336693	17.635597437332837	11.723580269950862	26.46031597934938
2	15.814196120443356	20.527629654795494	36.90608817202462	26.752086052736534
3	18.429113412320802	24.570000310974283	28.324937027707808	28.675949248997107
4	12.201961462661853	15.658193946131691	35.484515484515484	36.65532910669098
5	14.221964805622392	36.9374594277318	33.13480294025041	15.705772826395394
6	34.51032007830339	35.232402923471504	16.599353639451472	13.65792335877364
7	30.30707060027179	30.33249266880409	20.77014096420386	18.590295766720256
8	28.50892298363116	32.584769316286824	19.348278180963003	19.558029519119014
9	27.850594005754637	13.740071073890569	18.20873893211907	40.200595988235726
10	16.00418263661595	26.451678108017756	31.335800415153898	26.2083388402124
11	37.491924417994454	20.718503286596672	22.467882312240288	19.321689983168582
12	25.2179145507524	23.385037829903816	28.664587291764494	22.73246032757929
13	30.067356924842297	19.16391584427054	25.285046358419073	25.48368087246809
14	23.832273041427186	19.490608972689483	25.086120373386528	31.590997612496803
15	25.333729325108727	27.284558964160155	22.33245893788164	25.049252772849478
16	25.779186147940024	25.269109788086247	24.18349688014566	24.768207183828068
17	23.967005756889026	25.740951655348542	25.455633860303124	24.83640872745931
18	25.093506297632622	24.11253563579229	26.838698040935192	23.95526002563989
19	25.629332246999887	24.642302943984376	25.49281496154815	24.23554984746759
20	26.124740043925286	23.537968163884084	25.517016190161513	24.820275602029117
21	26.995883493937967	23.748158456329662	24.372825617961805	24.883132431770562
22	26.048737254962933	24.23864073669521	24.950613588745885	24.76200841959597
23	24.18431445719439	24.017787728954815	25.712053363186865	26.08584445066393
24	24.91706672367884	24.686556140794153	25.51670521467027	24.879671920856737
25	25.008104924762197	24.01623317304889	25.535138833279557	25.440523068909354
26	24.382797156909547	25.05197456706002	25.996302303052545	24.56892597297789
27	25.35202288275078	24.59576942783016	25.108516578565382	24.94369111085368
28	24.440107912270744	24.28974600188147	25.87709818617199	25.393047899675796
29	24.487114500113506	24.584995786206747	25.77294340455836	25.154946309121385
30	24.53095407657118	24.702852060443405	26.1165983013898	24.64959556159561
31	25.09590459766719	24.469473026200188	24.831554673006917	25.603067703125703
32	24.67873538867012	24.788123568993463	24.93102014001998	25.602120902316432
33	24.29328017514834	24.419152495988257	25.68650561644006	25.60106171242334
34	25.07418870559076	24.514964855117253	25.796911479676922	24.613934959615065
35	25.743495652106308	24.309254385794418	25.61887028621852	24.328379675880754
36	24.49412134245804	25.110521249780355	25.33793922769632	25.057418180065277
37	25.607257782998882	24.842466333860195	24.930786257233333	24.619489625907594
38	24.738371731396015	24.813711342771132	25.273679496429725	25.174237429403128
39	25.412914080284615	24.538882807349264	24.77811018920499	25.27009292316113
40	25.621103042930667	24.830594125604467	25.291698412451602	24.256604419013264
41	24.469444001725954	25.043440402100686	25.760411119188024	24.726704476985333
42	25.919078631786242	25.442270270186224	24.998969882473308	23.63968121555423
43	24.913062238341244	24.389530928696654	25.3342818159555	25.363125017006606
44	24.526167584179646	25.127286947422267	24.950496281098715	25.39604918729937
45	24.749370851839785	25.381278945285686	24.82719319454886	25.04215700832567
46	24.552484744637397	24.7836183566579	25.29984008010766	25.364056818597042
47	25.193137618473415	24.560683346044616	25.495720535288775	24.750458500193197
48	25.584076637634194	25.528644652990334	24.19143548626601	24.695843223109463
49	24.670639875237313	25.316532455557955	24.821773141503964	25.191054527700768
50	24.689567991839983	26.095335978704348	24.2261356851428	24.988960344312865
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	60.0
1	59.0
2	58.0
3	186.0
4	314.0
5	366.0
6	418.0
7	374.5
8	331.0
9	455.5
10	580.0
11	889.0
12	1198.0
13	2089.0
14	2980.0
15	3875.0
16	4770.0
17	5074.0
18	5378.0
19	5276.0
20	5174.0
21	5392.5
22	5611.0
23	6079.5
24	6548.0
25	6743.0
26	6938.0
27	8178.0
28	9418.0
29	11264.5
30	13111.0
31	15918.0
32	18725.0
33	22626.5
34	26528.0
35	30845.5
36	35163.0
37	40754.5
38	46346.0
39	49723.0
40	53100.0
41	58142.5
42	63185.0
43	64873.5
44	66562.0
45	75149.5
46	83737.0
47	89731.5
48	95726.0
49	98635.5
50	101545.0
51	96944.5
52	92344.0
53	91759.5
54	91175.0
55	92096.5
56	93018.0
57	90956.0
58	88894.0
59	81413.0
60	73932.0
61	65185.0
62	56438.0
63	49206.0
64	41974.0
65	35620.0
66	29266.0
67	25058.5
68	20851.0
69	18808.5
70	16766.0
71	13281.0
72	9796.0
73	8337.5
74	6879.0
75	5402.0
76	3925.0
77	3439.0
78	2953.0
79	2430.0
80	1907.0
81	1526.0
82	1145.0
83	990.5
84	836.0
85	593.5
86	351.0
87	251.5
88	152.0
89	109.0
90	66.0
91	54.0
92	42.0
93	29.5
94	17.0
95	21.5
96	26.0
97	19.5
98	13.0
99	14.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009717908550593376
2	3.10973073618988E-4
3	3.88716342023735E-4
4	0.0
5	0.0
6	4.6645961042848203E-4
7	7.7743268404747E-5
8	0.0
9	0.001399378831285446
10	0.001166149026071205
11	0.0
12	7.7743268404747E-5
13	2.3322980521424101E-4
14	3.10973073618988E-4
15	0.004897825909499061
16	5.442028788332291E-4
17	0.0
18	6.21946147237976E-4
19	7.7743268404747E-5
20	7.7743268404747E-4
21	0.0
22	0.0
23	3.88716342023735E-4
24	7.7743268404747E-4
25	0.00233229805214241
26	0.006141718203975013
27	0.005908488398760773
28	0.004275879762261085
29	0.002565527857356651
30	0.004431366299070579
31	0.008629502792926918
32	0.00310973073618988
33	0.0047423393726895675
34	0.0029542441993803863
35	0.00233229805214241
36	0.008318529719307929
37	0.004042649957046844
38	0.008474016256117423
39	0.0047423393726895675
40	0.001943581710118675
41	0.00310973073618988
42	0.0018658384417139281
43	0.00155486536809494
44	0.001399378831285446
45	0.001710351904904434
46	0.001243892294475952
47	0.002021324978523422
48	0.0018658384417139281
49	2.3322980521424101E-4
50	0.001010662489261711
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1286285.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.97518117692297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.30467356499572	40.95229087928537
2	13.227529735840825	14.014615685586076
3	4.116529342961176	6.542216632904639
4	1.7437893147764676	3.695102195386629
5	0.9118649744471012	2.4153106115112717
6	0.560554389554559	1.7817282207703333
7	0.3962503638693189	1.4694004372179172
8	0.26306601310880806	1.1148775764743923
9	0.19128005306681894	0.9119785920071557
>10	1.1112617700150742	10.576489906819532
>50	0.08412894773078794	3.1481561579937534
>100	0.07799299129927051	8.690322913315953
>500	0.008715116822776666	3.1413353103609216
>1k	0.0023634215112614686	1.5461748803662179
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1464	0.11381614494454961	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1448	0.11257225265007367	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1419	0.110317697866336	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1402	0.10899606230345531	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1306	0.10153270853659958	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1290	0.10028881624212363	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1288	0.10013332970531415	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	7.7743268404747E-5	0.0
11	0.0	0.0	0.0	7.7743268404747E-5	0.0
12	0.0	0.0	0.0	7.7743268404747E-5	3.88716342023735E-4
13	0.0	0.0	0.0	7.7743268404747E-5	5.44202878833229E-4
14	0.0	0.0	0.0	7.7743268404747E-5	5.44202878833229E-4
15	0.0	0.0	0.0	7.7743268404747E-5	0.001010662489261711
16	0.0	0.0	0.0	7.7743268404747E-5	0.001010662489261711
17	0.0	0.0	0.0	7.7743268404747E-5	0.001010662489261711
18	0.0	0.0	0.0	7.7743268404747E-5	0.001010662489261711
19	0.0	0.0	0.0	7.7743268404747E-5	0.001010662489261711
20	0.0	0.0	0.0	7.7743268404747E-5	0.0013216355628806991
21	0.0	0.0	0.0	5.44202878833229E-4	0.0013216355628806991
22	0.0	0.0	0.0	7.7743268404747E-4	0.0013216355628806991
23	0.0	0.0	0.0	0.0020990682469281693	0.001399378831285446
24	0.0	0.0	0.0	0.003964906688642097	0.0014771220996901932
25	0.0	0.0	0.0	0.005208798983118049	0.0014771220996901932
26	0.0	0.0	0.0	0.0069191508880224835	0.00155486536809494
27	0.0	0.0	0.0	0.008629502792926918	0.00155486536809494
28	0.0	0.0	0.0	0.012361179676354774	0.00155486536809494
29	0.0	0.0	0.0	0.018891614222353522	0.00155486536809494
30	0.0	0.0	0.0	0.032807659266803235	0.00155486536809494
31	0.0	0.0	0.0	0.0638272233602973	0.00155486536809494
32	0.0	0.0	0.0	0.09406935476974387	0.001710351904904434
33	0.0	0.0	0.0	0.1276544467205946	0.001710351904904434
34	0.0	0.0	0.0	0.15906272715611236	0.001710351904904434
35	0.0	0.0	0.0	0.19529109023272448	0.001710351904904434
36	0.0	0.0	0.0	0.2489339454319999	0.001710351904904434
37	0.0	0.0	0.0	0.3222458475376763	0.001710351904904434
38	0.0	0.0	0.0	0.4227678935850142	0.001710351904904434
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTAT	55	1.594727E-4	23.997711	8
TACCGTA	65	1.814848E-5	23.690046	7
CCCTATA	105	7.7548975E-8	20.950382	2
TGTAGGA	690	0.0	20.72266	2
AACGTAT	55	0.0044801887	20.002758	31
AGTATCG	55	0.004486241	19.998093	8
TTAGGAC	465	0.0	19.869844	3
TAATACT	200	0.0	19.79888	4
TAGGACG	560	0.0	19.641747	4
GTAGGAC	700	0.0	19.170343	3
CTATACT	115	2.0228435E-7	19.129354	4
GTATCGA	60	0.00741311	18.333008	9
TATGGCG	730	0.0	18.079763	16
GGACGTG	635	0.0	18.01473	6
CTGTAGG	830	0.0	17.493666	1
GATTTCG	240	0.0	17.417036	41
CGGTCCG	305	0.0	17.311169	39
TGGCGAG	750	0.0	17.304342	18
CATGGGT	950	0.0	17.135872	4
AGGACGT	645	0.0	17.053299	5
>>END_MODULE