FastQCFastQC Report
Thu 2 Feb 2017
SRR4063980_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063980_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079874
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT38590.357356506407229No Hit
GTATCAACGCAGAGTACTTTTTTTT32480.30077583125438706No Hit
GTCCTACAGTGGACATTTCTAAATT32110.297349505590467No Hit
CTGTAGGACGTGGAATATGGCAAGA31840.29484921388976865No Hit
CTTTAGGACGTGAAATATGGCGAGG30480.2822551519899544No Hit
GTCCTACAGTGTGCATTTCTCATTT25060.23206411118334175No Hit
TATCAACGCAGAGTACTTTTTTTTT23740.21984046286881614No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18410.17048285262910304No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG17690.16381540809390724No Hit
GATTAAGAGGGACGGCCGGGGGCAT17640.16335239111229644No Hit
CTGTAGGACCTGGAATATGGCGAGA17510.1621485469601083No Hit
CTGAAGGACCTGGAATATGGCGAGA16500.1527956039315698No Hit
ATTTAGAAATGTCCACTGTAGGACG16050.14862845109707243No Hit
GAATAGGACCGCGGTTCTATTTTGT15920.14742460694488432No Hit
GTATCTGATCGTCTTCGAACCTCCG15660.14501691864050806No Hit
GATATACACTGTTCTACAAATCCCG15580.14427609146993076No Hit
GCGCAAGACGGACCAGAGCGAAAGC15350.142146213354521No Hit
GAACTACGACGGTATCTGATCGTCT15000.13890509448324526No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14840.13742344014209065No Hit
GAATAACGCCGCCGCATCGCCAGTC14760.13668261297151335No Hit
GTCCTATTCCATTATTCCTAGCTGC14070.13029297862528405No Hit
TCGTAGTTCCGACCATAAACGATGC13910.12881132428412945No Hit
GTCCTTCAGTGTGCATTTCTCATTT13660.12649623937607535No Hit
ATCAGATACCGTCGTAGTTCCGACC13500.12501458503492074No Hit
GTACATGGGGAATAATTGCAATCCC13300.12316251710847746No Hit
GTACATGGGGTGGTATCAACGCAAA12810.11862495068869144No Hit
CTATTGGAGCTGGAATTACCGCGGC12740.11797672691443632No Hit
TTTCTAAATTTTCCACCTTTTTCAG12490.11566164200638224No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12320.11408738426890545No Hit
GGGTAGGCACACGCTGAGCCAGTCA12310.11399478087258329No Hit
CTCTTAATCATGGCCTCAGTTCCGA12070.11177229936085137No Hit
TTGTAGAACAGTGTATATCAATGAG12040.11149448917188486No Hit
GAATATGGCAAGAAAACTGAAAATC11980.1109388687939519No Hit
GCTTTGAACACTCTAATTTTTTCAA11980.1109388687939519No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG11960.11075366200130755No Hit
GAATAATGGAATAGGACCGCGGTTC11770.10899419747118645No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11720.10853118048957565No Hit
GGTATCAACGCAGAGTACTTTTTTT11690.10825337030060914No Hit
GTGCATGGCCGTTCTTAGTTGGTGG11410.10566047520358857No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT11190.10362320048450097No Hit
GTGTATATCAATGAGTTACAATGAG10980.10167852916173553No Hit
CTTTAATATACGCTATTGGAGCTGG10840.10038208161322523No Hit
CCATTATTCCTAGCTGCGGTATCCA10800.1000116680279366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGCG250.0060240319.00375617
GCTTTAT508.729777E-515.195961
TAGAAAT5200.014.6115014
GAATACG400.005274048514.25149313
AAATGTC5200.013.8847867
TCGCCAG2100.013.5741117
ATGTCCA5150.013.2823319
CGCCAGT2150.013.25781818
CCGACCA2800.013.2329179
CGCAAGG655.439379E-513.15644616
GGCGAAA655.441745E-513.15583719
AATGTCC5350.012.9633768
CGCGGTC1102.739398E-912.95530410
GAAATGT5600.012.8918196
GGTTCCG1550.012.8717218
TTTAGAA5450.012.7190322
CGAACGA1207.366907E-1012.66916916
AATGGCG604.0834132E-412.66916916
TTTTCGG1650.012.66916816
AGTGCAT604.088206E-412.66740810
Produced by FastQC (version 0.11.5)