##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063980_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1079874
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.247233473534877	32.0	32.0	32.0	32.0	32.0
2	30.867378045957214	32.0	32.0	32.0	32.0	32.0
3	30.882018642915746	32.0	32.0	32.0	32.0	32.0
4	30.90285903725805	32.0	32.0	32.0	32.0	32.0
5	30.854075568075533	32.0	32.0	32.0	32.0	32.0
6	34.50690265716185	36.0	36.0	36.0	32.0	36.0
7	34.412127711195936	36.0	36.0	36.0	32.0	36.0
8	34.37776907305852	36.0	36.0	36.0	32.0	36.0
9	34.49153327147427	36.0	36.0	36.0	32.0	36.0
10	34.20580641815619	36.0	36.0	36.0	32.0	36.0
11	34.46613030779517	36.0	36.0	36.0	32.0	36.0
12	34.270582493883545	36.0	36.0	36.0	32.0	36.0
13	34.3685568871924	36.0	36.0	36.0	32.0	36.0
14	34.271412220314595	36.0	36.0	36.0	32.0	36.0
15	34.208496546819354	36.0	36.0	36.0	32.0	36.0
16	34.21427407271589	36.0	36.0	36.0	32.0	36.0
17	34.13598623543117	36.0	36.0	36.0	32.0	36.0
18	34.135742688498844	36.0	36.0	36.0	32.0	36.0
19	34.11691641802655	36.0	36.0	36.0	32.0	36.0
20	34.092411707291774	36.0	36.0	36.0	32.0	36.0
21	34.0602301750019	36.0	36.0	36.0	32.0	36.0
22	34.02963864302687	36.0	36.0	36.0	32.0	36.0
23	34.00962149287787	36.0	36.0	36.0	32.0	36.0
24	33.97214026821648	36.0	36.0	36.0	32.0	36.0
25	33.61727201506842	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	7.0
4	14.0
5	22.0
6	40.0
7	18.0
8	53.0
9	55.0
10	99.0
11	30.0
12	67.0
13	60.0
14	123.0
15	184.0
16	321.0
17	410.0
18	554.0
19	740.0
20	1135.0
21	1732.0
22	2572.0
23	4121.0
24	5974.0
25	8679.0
26	12216.0
27	15947.0
28	21484.0
29	29176.0
30	38078.0
31	51650.0
32	72257.0
33	101943.0
34	222122.0
35	487990.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.143163511554334	18.572255700740644	11.76119651864581	25.523384269059207
2	14.755074560241823	20.580886425053574	41.549252730603314	23.114786284101285
3	18.56535629948604	25.76543038384961	29.28258554428856	26.386627772375792
4	11.212979941101295	16.439776999870347	38.27221203533922	34.075031023689135
5	12.849754944635642	38.658548783243624	34.550676263896655	13.941020008224081
6	31.486009387584236	38.151416473098685	18.061838025273126	12.30073611404396
7	28.540081621159995	31.207311447895652	21.873303804479715	18.379303126464634
8	25.921849915747018	36.593307747105804	19.897414234724454	17.587428102422724
9	27.631335141785623	13.767676879975097	19.632639599175818	38.968348379063464
10	15.940880838809388	27.28975097382983	32.355241261475626	24.414126925885157
11	36.54028791709945	20.523706323446703	25.202058059668165	17.733947699785688
12	25.68943613617389	23.830893670095506	30.761347283444174	19.71832291028643
13	30.054577970514924	20.576265532482697	25.91137797792789	23.45777851907449
14	21.933232943356888	22.72359550561798	25.71972663037183	29.62344492065331
15	23.056139047154872	32.414376532920194	22.2517700103129	22.277714409612027
16	22.673428897881724	26.193854128924105	28.647495067790658	22.485221905403517
17	21.493766551949726	28.128353246637715	27.42494750634279	22.952932695069766
18	22.402128602117852	25.146475777049133	31.346279672813615	21.105115948019403
19	25.353410103998115	24.478294957268286	26.230193372367378	23.938101566366218
20	25.36570763407735	25.605743815592895	26.68127272929479	22.347275821034955
21	25.920842654666167	24.485805316820286	24.808306442794045	24.7850455857195
22	25.434077253934845	27.28243311248335	25.67540891536988	21.608080718211923
23	22.13186772447542	26.903499666345372	27.61214502854601	23.352487580633202
24	24.619147267999104	25.907809450374693	27.687933376952916	21.785109904673288
25	23.21472758799973	26.668081545195637	27.697081303307016	22.42010956349762
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	157.0
1	157.0
2	374.5
3	592.0
4	592.0
5	592.0
6	1558.0
7	2524.0
8	2524.0
9	2524.0
10	2503.5
11	2483.0
12	2483.0
13	2483.0
14	2739.0
15	2995.0
16	2995.0
17	2995.0
18	5501.5
19	8008.0
20	8008.0
21	8008.0
22	15034.5
23	22061.0
24	22061.0
25	22061.0
26	36691.5
27	51322.0
28	51322.0
29	51322.0
30	67950.5
31	84579.0
32	84579.0
33	84579.0
34	93332.5
35	102086.0
36	102086.0
37	102086.0
38	106434.0
39	110782.0
40	110782.0
41	110782.0
42	117098.5
43	123415.0
44	123415.0
45	123415.0
46	130205.5
47	136996.0
48	136996.0
49	136996.0
50	130445.5
51	123895.0
52	123895.0
53	123895.0
54	105647.5
55	87400.0
56	87400.0
57	87400.0
58	80102.5
59	72805.0
60	72805.0
61	72805.0
62	65729.0
63	58653.0
64	58653.0
65	58653.0
66	49494.5
67	40336.0
68	40336.0
69	40336.0
70	30334.5
71	20333.0
72	20333.0
73	20333.0
74	16498.0
75	12663.0
76	12663.0
77	12663.0
78	10736.5
79	8810.0
80	8810.0
81	8810.0
82	5991.0
83	3172.0
84	3172.0
85	3172.0
86	2513.5
87	1855.0
88	1855.0
89	1855.0
90	1395.5
91	936.0
92	936.0
93	936.0
94	645.5
95	355.0
96	355.0
97	355.0
98	508.0
99	661.0
100	661.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037874789095764874
2	0.007037858120484427
3	0.0022224815117319243
4	0.00500058340139683
5	0.010927200766015294
6	0.013890509448324527
7	0.023336055873185206
8	0.03583751437667728
9	0.04611649136843743
10	0.05834013968296301
11	0.056025054774908926
12	0.06435936044390364
13	0.06334072308435984
14	0.06935994384530048
15	0.05954398383515114
16	0.06880432346736749
17	0.0644519638402258
18	0.07926850725177197
19	0.07556437139888542
20	0.08056495480028225
21	0.07482354422830811
22	0.07973152423338278
23	0.08463950423845745
24	0.07852768008119466
25	0.08010193781867143
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1079874.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.59031902981105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.77336385437516	32.27211740519791
2	14.43107039868717	12.292477844434982
3	4.483054806561174	5.728042033187047
4	1.831923860956049	3.1208888670576536
5	0.9121148554585684	1.9423631342905212
6	0.506727668763346	1.2949015844317993
7	0.3355908443512612	1.0005044787082713
8	0.22296591752175068	0.7596951648020718
9	0.1636296068908845	0.6272133444184804
>10	0.9808284953332658	8.097782238977484
>50	0.16232648071626013	4.874619810073211
>100	0.16428833618983188	14.785731400972027
>500	0.02228379025797832	6.433975560425225
>1k	0.009831083937343377	6.769687133023336
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	3859	0.357356506407229	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3248	0.30077583125438706	No Hit
GTCCTACAGTGGACATTTCTAAATT	3211	0.297349505590467	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3184	0.29484921388976865	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3048	0.2822551519899544	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2506	0.23206411118334175	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2374	0.21984046286881614	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1841	0.17048285262910304	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1769	0.16381540809390724	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1764	0.16335239111229644	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1751	0.1621485469601083	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1650	0.1527956039315698	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1605	0.14862845109707243	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1592	0.14742460694488432	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1566	0.14501691864050806	No Hit
GATATACACTGTTCTACAAATCCCG	1558	0.14427609146993076	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1535	0.142146213354521	No Hit
GAACTACGACGGTATCTGATCGTCT	1500	0.13890509448324526	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1484	0.13742344014209065	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1476	0.13668261297151335	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1407	0.13029297862528405	No Hit
TCGTAGTTCCGACCATAAACGATGC	1391	0.12881132428412945	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1366	0.12649623937607535	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1350	0.12501458503492074	No Hit
GTACATGGGGAATAATTGCAATCCC	1330	0.12316251710847746	No Hit
GTACATGGGGTGGTATCAACGCAAA	1281	0.11862495068869144	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1274	0.11797672691443632	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1249	0.11566164200638224	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1232	0.11408738426890545	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1231	0.11399478087258329	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1207	0.11177229936085137	No Hit
TTGTAGAACAGTGTATATCAATGAG	1204	0.11149448917188486	No Hit
GAATATGGCAAGAAAACTGAAAATC	1198	0.1109388687939519	No Hit
GCTTTGAACACTCTAATTTTTTCAA	1198	0.1109388687939519	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1196	0.11075366200130755	No Hit
GAATAATGGAATAGGACCGCGGTTC	1177	0.10899419747118645	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1172	0.10853118048957565	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1169	0.10825337030060914	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	1141	0.10566047520358857	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	1119	0.10362320048450097	No Hit
GTGTATATCAATGAGTTACAATGAG	1098	0.10167852916173553	No Hit
CTTTAATATACGCTATTGGAGCTGG	1084	0.10038208161322523	No Hit
CCATTATTCCTAGCTGCGGTATCCA	1080	0.1000116680279366	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.260339632216352E-5	0.0
10	0.0	0.0	0.0	9.260339632216352E-5	0.0
11	0.0	0.0	0.0	9.260339632216352E-5	0.0
12	0.0	0.0	0.0	9.260339632216352E-5	0.0
13	0.0	0.0	0.0	9.260339632216352E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAAGCG	25	0.00602403	19.003756	17
GCTTTAT	50	8.729777E-5	15.19596	1
TAGAAAT	520	0.0	14.611501	4
GAATACG	40	0.0052740485	14.251493	13
AAATGTC	520	0.0	13.884786	7
TCGCCAG	210	0.0	13.57411	17
ATGTCCA	515	0.0	13.282331	9
CGCCAGT	215	0.0	13.257818	18
CCGACCA	280	0.0	13.232917	9
CGCAAGG	65	5.439379E-5	13.156446	16
GGCGAAA	65	5.441745E-5	13.155837	19
AATGTCC	535	0.0	12.963376	8
CGCGGTC	110	2.739398E-9	12.955304	10
GAAATGT	560	0.0	12.891819	6
GGTTCCG	155	0.0	12.871721	8
TTTAGAA	545	0.0	12.719032	2
CGAACGA	120	7.366907E-10	12.669169	16
AATGGCG	60	4.0834132E-4	12.669169	16
TTTTCGG	165	0.0	12.669168	16
AGTGCAT	60	4.088206E-4	12.667408	10
>>END_MODULE