FastQCFastQC Report
Thu 2 Feb 2017
SRR4063980_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063980_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079874
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18240.16890859489162624No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA16860.1561293261991677No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16730.15492548204697956No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16590.15362903449846926No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16220.15020270883454923No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG16080.14890626128603893No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA15530.14381307448831993No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC14660.13575657900829172No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC14160.13112640919218352No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA14100.13057078881425055No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13390.12399594767537694No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA13200.12223648314525584No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA12900.11945838125559093No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG12750.11806933031075849No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12720.11779152012179198No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG12450.11529122842109357No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT12250.1134391604946503No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11480.10630869897784372No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC11380.10538266501462208No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA11330.10491964803301125No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG11240.10408621746611178No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10930.10121551218012471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGG609.749252E-625.66750535
AGGACCT17150.023.2172685
GGACCTG16950.022.452936
TAGGACC16650.021.5362454
CGGACCG956.862756E-720.84278521
TGTAGGA26400.020.8320832
GTCCTAC22950.020.1313761
CTGTAGG25950.020.0931421
GTAGGAC27900.020.0274723
TTAGGAC16200.019.9617653
TCCTACA23500.019.5647832
GTATCAA29450.019.4980891
GTCCTAA12900.019.4424951
AAATTTA11450.019.40674644
CCTACAG22650.018.9392623
GATATAC7900.018.9373261
TAGGACG26050.018.9163324
CTTTAGG17050.018.8393251
GACCTGG20250.018.7939367
AGGACGT26000.018.7834875
Produced by FastQC (version 0.11.5)