##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063980_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1079874
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.321564367694748	32.0	32.0	32.0	32.0	32.0
2	31.459638809712985	32.0	32.0	32.0	32.0	32.0
3	31.516581564145447	32.0	32.0	32.0	32.0	32.0
4	31.58764170634722	32.0	32.0	32.0	32.0	32.0
5	31.547661116019093	32.0	32.0	32.0	32.0	32.0
6	35.13015685163269	36.0	36.0	36.0	36.0	36.0
7	35.146918066367	36.0	36.0	36.0	36.0	36.0
8	35.099018959619364	36.0	36.0	36.0	36.0	36.0
9	35.199375112281615	36.0	36.0	36.0	36.0	36.0
10	35.066633699857576	36.0	36.0	36.0	36.0	36.0
11	35.19998629469735	36.0	36.0	36.0	36.0	36.0
12	35.09956994982748	36.0	36.0	36.0	36.0	36.0
13	35.14817747255698	36.0	36.0	36.0	36.0	36.0
14	35.09353683855709	36.0	36.0	36.0	36.0	36.0
15	35.06771993769644	36.0	36.0	36.0	36.0	36.0
16	35.07636168664122	36.0	36.0	36.0	36.0	36.0
17	35.04497654355971	36.0	36.0	36.0	36.0	36.0
18	35.04165856386949	36.0	36.0	36.0	36.0	36.0
19	35.026130826374185	36.0	36.0	36.0	36.0	36.0
20	35.011453188057125	36.0	36.0	36.0	36.0	36.0
21	34.9981544143113	36.0	36.0	36.0	36.0	36.0
22	34.981596001014935	36.0	36.0	36.0	36.0	36.0
23	34.93866228837809	36.0	36.0	36.0	36.0	36.0
24	34.90199967774018	36.0	36.0	36.0	36.0	36.0
25	34.86292289656016	36.0	36.0	36.0	32.0	36.0
26	34.80775164509934	36.0	36.0	36.0	32.0	36.0
27	34.79323976686169	36.0	36.0	36.0	32.0	36.0
28	34.74905035217072	36.0	36.0	36.0	32.0	36.0
29	34.71181915667939	36.0	36.0	36.0	32.0	36.0
30	34.666766678334696	36.0	36.0	36.0	32.0	36.0
31	34.648543255972456	36.0	36.0	36.0	32.0	36.0
32	34.613656778476006	36.0	36.0	36.0	32.0	36.0
33	34.61640987744867	36.0	36.0	36.0	32.0	36.0
34	34.59360536507037	36.0	36.0	36.0	32.0	36.0
35	34.55709184590054	36.0	36.0	36.0	32.0	36.0
36	34.499756453067675	36.0	36.0	36.0	32.0	36.0
37	34.457962688239554	36.0	36.0	36.0	32.0	36.0
38	34.38955007713863	36.0	36.0	36.0	32.0	36.0
39	34.321948671789485	36.0	36.0	36.0	32.0	36.0
40	34.330983059134674	36.0	36.0	36.0	32.0	36.0
41	34.31984750072694	36.0	36.0	36.0	32.0	36.0
42	34.24209583710692	36.0	36.0	36.0	32.0	36.0
43	34.247923368837476	36.0	36.0	36.0	32.0	36.0
44	34.12161696642386	36.0	36.0	36.0	32.0	36.0
45	34.111474116424695	36.0	36.0	36.0	32.0	36.0
46	33.996143068543184	36.0	36.0	36.0	32.0	36.0
47	33.98777449961755	36.0	36.0	36.0	32.0	36.0
48	33.92041201103092	36.0	36.0	36.0	32.0	36.0
49	33.88154173542469	36.0	36.0	36.0	32.0	36.0
50	33.334260293330516	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	4.0
20	15.0
21	24.0
22	120.0
23	355.0
24	910.0
25	2070.0
26	4381.0
27	7977.0
28	13474.0
29	20476.0
30	29183.0
31	41092.0
32	59238.0
33	93786.0
34	189172.0
35	617595.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.32394235756094	19.53563754908498	12.266244350596429	25.87417574275765
2	14.147773890464189	21.35487180816879	40.99499382330771	23.502360478059316
3	17.894114934311478	26.230311269237287	29.745459865965223	26.130113930486015
4	10.990448885703332	17.079029590489263	38.35688237701806	33.57363914678935
5	12.356997205229499	39.146696744249795	34.47004002318789	14.026266027332818
6	31.60294146245246	38.4211632685756	17.72683959472937	12.249055674242571
7	28.200379304213836	31.485305665856693	22.05131719314882	18.262997836780656
8	25.79664460543045	36.7748800090381	19.861039214796556	17.567436170734894
9	27.58625479579165	13.599999259158421	19.547660653811214	39.26608529123872
10	15.817254934644609	27.449449699730984	32.33061540099919	24.402679964625207
11	36.46342073241878	20.628980788499398	25.234703307978524	17.672895171103296
12	25.55439389631929	23.938463134090767	30.792232049509526	19.714910920080417
13	30.089825627158824	20.519877392649118	25.96321779473455	23.427079185457508
14	21.885452666437008	22.777969159193532	25.708975541455064	29.627602632914396
15	22.907095303229127	32.52965521328879	22.304961813327505	22.258287670154573
16	22.697302623293304	26.319795826141068	28.45562033737582	22.52728121318981
17	21.64687732087262	28.21403237785149	27.200580808501734	22.938509492774156
18	22.47770564975414	25.080611555094595	31.43498754479706	21.00669525035421
19	25.544554272072485	24.482208109464622	26.343258565351142	23.629979053111754
20	25.472673949662322	25.63667562764674	26.701806796577728	22.188843626113215
21	25.954972524572312	24.391271574276256	25.031994473429307	24.62176142772212
22	25.39585673500495	27.187641509696046	25.84100167334492	21.575500081954083
23	22.09024082617658	26.820272144877233	27.822526128241837	23.266960900704348
24	24.756823030877733	25.661282077875896	27.723193119184497	21.858701772061874
25	23.28062563955346	26.464108607174115	27.754096614823286	22.501169138449143
26	21.20456934353888	28.448284643986643	28.888271493464096	21.458874519010376
27	22.96315504331841	26.93113044605689	27.170342518718833	22.93537199190587
28	21.415652692514634	27.47338231031452	27.243160246965488	23.86780475020536
29	22.664704651236015	25.817851130072732	26.76178552047429	24.755658698216962
30	21.36280710852634	26.62039395089968	28.19304890584629	23.823750034727688
31	24.303932282501993	25.481857415399432	26.56605975291263	23.648150549185946
32	22.721834201056083	27.29316804928147	26.37071486648923	23.614282883173214
33	21.51685268394596	27.125523694514314	28.53889693543787	22.81872668610186
34	23.32602670207929	26.885069190741973	28.23573100113813	21.553173106040614
35	23.345344294145374	27.11300767608745	27.922566808075587	21.61908122169159
36	21.276934660382725	27.525748085968647	28.024827072696613	23.172490180952018
37	22.973680043192104	27.2489945148621	25.899148659924226	23.878176782021566
38	22.54547037375587	28.019141131112303	26.549474933990513	22.88591356114132
39	24.73217758515308	25.570002407808705	26.22872330573614	23.46909670130207
40	25.47947006865845	26.055389789949974	27.461989668820692	21.003150472570887
41	23.751979478251208	25.90463313669744	28.63459989072354	21.70878749432781
42	24.26402360310337	28.473465955708992	26.982343974855954	20.280166466331686
43	22.118875109273826	27.40819146256538	27.977063608481128	22.495869819679662
44	21.46144599958143	28.16785848562131	26.61701217377776	23.753683341019503
45	22.006576784561688	28.417876778357176	26.71469145508812	22.860854981993022
46	22.400557106159468	26.20658489108834	26.942416929507925	24.45044107324427
47	24.238868721292043	25.935879389539192	26.952141057934508	22.873110831234257
48	23.328845063953207	27.34967384419346	25.495901290176803	23.825579801676525
49	23.425504921888745	27.202070619611618	25.952290553492553	23.420133905007084
50	22.552914462669097	29.17351943222059	25.131776829503195	23.14178927560712
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23.0
1	20.5
2	18.0
3	52.5
4	87.0
5	129.5
6	172.0
7	168.5
8	165.0
9	238.0
10	311.0
11	464.5
12	618.0
13	1197.0
14	1776.0
15	2507.0
16	3238.0
17	3752.0
18	4266.0
19	4450.0
20	4634.0
21	4958.0
22	5282.0
23	5803.5
24	6325.0
25	7617.5
26	8910.0
27	12570.5
28	16231.0
29	21219.5
30	26208.0
31	31603.0
32	36998.0
33	45964.5
34	54931.0
35	64723.0
36	74515.0
37	77450.5
38	80386.0
39	74848.0
40	69310.0
41	66373.0
42	63436.0
43	58434.5
44	53433.0
45	56118.5
46	58804.0
47	61374.5
48	63945.0
49	66092.0
50	68239.0
51	62191.5
52	56144.0
53	54008.5
54	51873.0
55	53506.5
56	55140.0
57	54954.5
58	54769.0
59	48682.0
60	42595.0
61	37007.5
62	31420.0
63	27470.0
64	23520.0
65	20011.0
66	16502.0
67	14907.0
68	13312.0
69	12626.5
70	11941.0
71	9126.0
72	6311.0
73	5653.5
74	4996.0
75	3955.0
76	2914.0
77	2579.0
78	2244.0
79	1901.5
80	1559.0
81	1226.0
82	893.0
83	819.0
84	745.0
85	538.5
86	332.0
87	259.5
88	187.0
89	135.5
90	84.0
91	75.5
92	67.0
93	48.5
94	30.0
95	24.5
96	19.0
97	11.5
98	4.0
99	8.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01055678718072664
2	7.408271705773081E-4
3	4.6301698161081755E-4
4	0.0
5	0.0
6	2.7781018896649054E-4
7	1.8520679264432703E-4
8	9.260339632216352E-5
9	0.0019446713227654338
10	8.334305668994715E-4
11	0.0
12	9.260339632216352E-5
13	3.7041358528865407E-4
14	5.556203779329811E-4
15	0.004352359627141685
16	9.260339632216351E-4
17	0.0
18	3.7041358528865407E-4
19	0.0
20	6.482237742551446E-4
21	0.0
22	9.260339632216352E-5
23	7.408271705773081E-4
24	1.8520679264432703E-4
25	0.0017594645301211068
26	0.006389634346229283
27	0.007315668309450917
28	0.004352359627141685
29	0.0029633086823092326
30	0.004074549438175194
31	0.00870471925428337
32	0.003333722267597886
33	0.0042597562308195214
34	0.0030559120786313957
35	0.002500291700698415
36	0.007871288687383899
37	0.004352359627141685
38	0.009723356613827169
39	0.00500058340139683
40	0.003333722267597886
41	0.004074549438175194
42	0.0014816543411546163
43	0.001666861133798943
44	0.0011112407558659622
45	0.0013890509448324527
46	7.408271705773081E-4
47	0.0031485154749535593
48	0.002037274719087597
49	3.7041358528865407E-4
50	0.001666861133798943
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1079874.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.0467733925833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.41705139542505	38.74473627908812
2	13.837307522068507	13.850251878409017
3	4.1620328863600715	6.248889501484257
4	1.7228181489240795	3.44885957983333
5	0.7741864954844875	1.9372768051555178
6	0.446120419850837	1.3396132534847378
7	0.2856356866515153	1.000660113787832
8	0.1838851470989884	0.7362286629699935
9	0.1384037490739659	0.6233994959929858
>10	0.784733460098286	7.588383400569938
>50	0.11058592509234236	3.92957519709769
>100	0.11840607239956115	12.49503408468894
>500	0.013984968915139779	4.820089020441804
>1k	0.004848122557248456	3.2370027269958928
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1824	0.16890859489162624	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1686	0.1561293261991677	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1673	0.15492548204697956	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1659	0.15362903449846926	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1622	0.15020270883454923	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1608	0.14890626128603893	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1553	0.14381307448831993	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1466	0.13575657900829172	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1416	0.13112640919218352	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	1410	0.13057078881425055	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1339	0.12399594767537694	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1320	0.12223648314525584	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	1290	0.11945838125559093	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1275	0.11806933031075849	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	1272	0.11779152012179198	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG	1245	0.11529122842109357	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	1225	0.1134391604946503	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1148	0.10630869897784372	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	1138	0.10538266501462208	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA	1133	0.10491964803301125	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	1124	0.10408621746611178	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1093	0.10121551218012471	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	1.8520679264432703E-4	0.0
21	0.0	0.0	0.0	5.556203779329811E-4	0.0
22	0.0	0.0	0.0	5.556203779329811E-4	0.0
23	0.0	0.0	0.0	0.001296447548510289	0.0
24	0.0	0.0	0.0	0.0019446713227654338	0.0
25	0.0	0.0	0.0	0.0025002917006984146	0.0
26	0.0	0.0	0.0	0.0027781018896649054	0.0
27	0.0	0.0	0.0	0.0036115324565643768	0.0
28	0.0	0.0	0.0	0.00527839359036332	0.0
29	0.0	0.0	0.0	0.007778685291061735	0.0
30	0.0	0.0	0.0	0.014631336618901834	0.0
31	0.0	0.0	0.0	0.033244619279656704	0.0
32	0.0	0.0	0.0	0.05028364420293479	0.0
33	0.0	0.0	0.0	0.06880432346736749	0.0
34	0.0	0.0	0.0	0.08834364009134399	0.0
35	0.0	0.0	0.0	0.11251312653142867	0.0
36	0.0	0.0	0.0	0.14640596958534052	0.0
37	0.0	0.0	0.0	0.19604139001402016	0.0
38	0.0	0.0	0.0	0.26799422895634123	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGG	60	9.749252E-6	25.667505	35
AGGACCT	1715	0.0	23.217268	5
GGACCTG	1695	0.0	22.45293	6
TAGGACC	1665	0.0	21.536245	4
CGGACCG	95	6.862756E-7	20.842785	21
TGTAGGA	2640	0.0	20.832083	2
GTCCTAC	2295	0.0	20.131376	1
CTGTAGG	2595	0.0	20.093142	1
GTAGGAC	2790	0.0	20.027472	3
TTAGGAC	1620	0.0	19.961765	3
TCCTACA	2350	0.0	19.564783	2
GTATCAA	2945	0.0	19.498089	1
GTCCTAA	1290	0.0	19.442495	1
AAATTTA	1145	0.0	19.406746	44
CCTACAG	2265	0.0	18.939262	3
GATATAC	790	0.0	18.937326	1
TAGGACG	2605	0.0	18.916332	4
CTTTAGG	1705	0.0	18.839325	1
GACCTGG	2025	0.0	18.793936	7
AGGACGT	2600	0.0	18.783487	5
>>END_MODULE