FastQCFastQC Report
Thu 2 Feb 2017
SRR4063979_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063979_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2539119
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38930.15332089594855539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9000.014.2453881
GTATCAA27800.013.220541
TTGCGCG2400.012.27290918
TAGGACG5250.011.9408754
CGCATCG2550.011.92217413
ACGGTAT2950.011.9170339
GTATTAG3800.011.7461971
AACCGCG1950.011.6931337
GCGTTAT2200.011.6553181
CGTCGTA4350.011.35667510
CGGTCCA3350.011.343636510
AACGGAC855.316538E-511.17836115
CGCTTAC855.316538E-511.17836118
GAACCGC2550.011.1759386
AGAACCG2650.011.11185
TCGCGTA1900.011.0016439
CGTTATT2250.010.9714152
CGGAATT1502.1282176E-910.76848815
GTCGCGT1951.8189894E-1210.7193398
GTAGGAC6650.010.7106083
Produced by FastQC (version 0.11.5)