##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063979_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2539119
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22099161165743	32.0	32.0	32.0	32.0	32.0
2	30.727670503036684	32.0	32.0	32.0	32.0	32.0
3	30.751668984399707	32.0	32.0	32.0	32.0	32.0
4	30.794535427445503	32.0	32.0	32.0	32.0	32.0
5	30.65174968168093	32.0	32.0	32.0	32.0	32.0
6	34.315625616601665	36.0	36.0	36.0	32.0	36.0
7	34.2536572724634	36.0	36.0	36.0	32.0	36.0
8	34.2116426996923	36.0	36.0	36.0	32.0	36.0
9	34.39497361092568	36.0	36.0	36.0	32.0	36.0
10	34.01400997747644	36.0	36.0	36.0	32.0	36.0
11	34.359727921377456	36.0	36.0	36.0	32.0	36.0
12	34.13868550469671	36.0	36.0	36.0	32.0	36.0
13	34.24397084185499	36.0	36.0	36.0	32.0	36.0
14	34.126092554149686	36.0	36.0	36.0	32.0	36.0
15	34.03996110461936	36.0	36.0	36.0	32.0	36.0
16	34.04538779001693	36.0	36.0	36.0	32.0	36.0
17	33.965681009830575	36.0	36.0	36.0	32.0	36.0
18	33.95764003183781	36.0	36.0	36.0	32.0	36.0
19	33.978998227337904	36.0	36.0	36.0	32.0	36.0
20	33.962258562910996	36.0	36.0	36.0	32.0	36.0
21	33.94679965767654	36.0	36.0	36.0	32.0	36.0
22	33.92173190779952	36.0	36.0	36.0	32.0	36.0
23	33.85875100773142	36.0	36.0	36.0	32.0	36.0
24	33.83489942771489	36.0	36.0	36.0	32.0	36.0
25	33.413225217093014	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	18.0
5	27.0
6	103.0
7	41.0
8	146.0
9	155.0
10	215.0
11	78.0
12	155.0
13	143.0
14	245.0
15	439.0
16	775.0
17	1000.0
18	1311.0
19	1737.0
20	2713.0
21	4057.0
22	6443.0
23	10130.0
24	15005.0
25	22236.0
26	31450.0
27	41241.0
28	56300.0
29	75133.0
30	99209.0
31	134556.0
32	187589.0
33	263086.0
34	543449.0
35	1039928.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.38398560837022	17.154792757852004	11.326592444394363	26.134629189383414
2	16.81602472176786	19.378907331574883	37.29285456307797	26.51221338357929
3	19.020278918825248	23.06641355761316	28.4247899838131	29.488517539748493
4	12.69489180346722	15.391110881802824	35.73276009762756	36.18123721710239
5	14.793414470833827	36.23939501358856	33.72555043522785	15.241640080349757
6	34.683619591184005	35.474703550306344	16.549938061166813	13.291738797342834
7	30.648912426051357	30.182471659419853	20.446500532186025	18.722115382342768
8	28.285101378703086	33.09115908370121	19.050725793901716	19.573013743693995
9	27.32841452002167	14.095729695118948	18.26776338472147	40.308092400137916
10	15.877884735119016	26.65466877463876	31.52402126676933	25.94342522347289
11	37.464411770153134	20.88188273902385	22.427924106289655	19.22578138453336
12	24.70612436469307	23.377442756860646	28.886411436529603	23.03002144191668
13	29.633843469186527	19.414390733534738	25.104216995192896	25.84754880208584
14	23.68135954186951	19.469639318006355	24.844301921264833	32.0046992188593
15	25.30260828718744	27.413457734146068	21.848286809131764	25.435647169534725
16	25.98517702288789	25.64479083412548	23.61051338923281	24.759518753753827
17	24.35150545944069	25.756650854708766	25.046472338663055	24.845371347187484
18	25.014268011590985	24.975484245277364	25.888321671927173	24.121926071204474
19	25.814526265257403	24.662868007430205	24.95976881701995	24.562836910292443
20	26.016661358827776	24.285294238209502	24.581416467754092	25.11662793520863
21	26.834654470583413	24.200577958133508	24.1545825174759	24.810185053807178
22	26.201247717877653	24.22687685841224	24.6395902112323	24.932285212477808
23	24.829601891998422	24.242885297595585	25.102286164761527	25.825226645644463
24	24.966399518202593	24.796406699443395	24.994462325287348	25.242731457066665
25	25.200207795156228	24.36898775537875	24.977237916782613	25.45356653268241
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	63.0
1	63.0
2	250.5
3	438.0
4	438.0
5	438.0
6	1268.5
7	2099.0
8	2099.0
9	2099.0
10	2647.0
11	3195.0
12	3195.0
13	3195.0
14	4544.5
15	5894.0
16	5894.0
17	5894.0
18	10240.5
19	14587.0
20	14587.0
21	14587.0
22	23878.0
23	33169.0
24	33169.0
25	33169.0
26	50659.0
27	68149.0
28	68149.0
29	68149.0
30	87472.5
31	106796.0
32	106796.0
33	106796.0
34	132200.5
35	157605.0
36	157605.0
37	157605.0
38	181789.5
39	205974.0
40	205974.0
41	205974.0
42	236498.5
43	267023.0
44	267023.0
45	267023.0
46	295865.0
47	324707.0
48	324707.0
49	324707.0
50	334059.0
51	343411.0
52	343411.0
53	343411.0
54	321629.5
55	299848.0
56	299848.0
57	299848.0
58	277686.5
59	255525.0
60	255525.0
61	255525.0
62	223451.5
63	191378.0
64	191378.0
65	191378.0
66	158543.0
67	125708.0
68	125708.0
69	125708.0
70	95289.0
71	64870.0
72	64870.0
73	64870.0
74	49845.0
75	34820.0
76	34820.0
77	34820.0
78	26964.0
79	19108.0
80	19108.0
81	19108.0
82	13305.0
83	7502.0
84	7502.0
85	7502.0
86	5574.5
87	3647.0
88	3647.0
89	3647.0
90	2602.5
91	1558.0
92	1558.0
93	1558.0
94	1104.5
95	651.0
96	651.0
97	651.0
98	1022.5
99	1394.0
100	1394.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03847791300840961
2	0.005592490938786248
3	0.0011421284311605717
4	0.003229466598454031
5	0.008625038842212595
6	0.012760331437793976
7	0.0209915328899512
8	0.03402755050078393
9	0.04899337132288798
10	0.05714580529703413
11	0.055609839475818185
12	0.06167493528267088
13	0.06399857588399756
14	0.06600714657327994
15	0.05966636459338849
16	0.0672280424824516
17	0.06254137754079268
18	0.07778288453593549
19	0.07514417402256451
20	0.07841302436002409
21	0.07526232523958112
22	0.07904316418411268
23	0.08345414295273282
24	0.07691644227781369
25	0.0789643967061016
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2539119.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.30446340691956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.68664668608925	30.87429488054773
2	15.511922175176737	13.744947767622035
3	6.03808028849759	8.025417215693514
4	2.8772508209058483	5.099002148294097
5	1.6271928637030537	3.60459533429663
6	0.9812353200880376	2.6083862599450476
7	0.6513335924381535	2.019988971831154
8	0.42757959801040013	1.5154947722877719
9	0.31829011209447233	1.2691505361666486
>10	1.6662297713536136	13.372306977086454
>50	0.11809072262582135	3.6353195998730268
>100	0.07982175609261237	7.367032370903005
>500	0.011062624713895321	3.311812946677226
>1k	0.0052636682106426205	3.552250218775726
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3893	0.15332089594855539	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1815121701661087E-4
2	0.0	0.0	0.0	0.0	1.1815121701661087E-4
3	0.0	0.0	0.0	0.0	1.1815121701661087E-4
4	0.0	0.0	0.0	3.938373900553696E-5	1.1815121701661087E-4
5	0.0	0.0	0.0	3.938373900553696E-5	1.1815121701661087E-4
6	0.0	0.0	0.0	3.938373900553696E-5	1.5753495602214784E-4
7	0.0	0.0	0.0	3.938373900553696E-5	1.5753495602214784E-4
8	0.0	0.0	0.0	3.938373900553696E-5	1.5753495602214784E-4
9	0.0	0.0	0.0	3.938373900553696E-5	1.5753495602214784E-4
10	0.0	0.0	0.0	3.938373900553696E-5	1.5753495602214784E-4
11	0.0	0.0	0.0	3.938373900553696E-5	1.5753495602214784E-4
12	0.0	0.0	0.0	3.938373900553696E-5	4.726048680664435E-4
13	0.0	0.0	0.0	3.938373900553696E-5	5.513723460775174E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	900	0.0	14.245388	1
GTATCAA	2780	0.0	13.22054	1
TTGCGCG	240	0.0	12.272909	18
TAGGACG	525	0.0	11.940875	4
CGCATCG	255	0.0	11.922174	13
ACGGTAT	295	0.0	11.917033	9
GTATTAG	380	0.0	11.746197	1
AACCGCG	195	0.0	11.693133	7
GCGTTAT	220	0.0	11.655318	1
CGTCGTA	435	0.0	11.356675	10
CGGTCCA	335	0.0	11.3436365	10
AACGGAC	85	5.316538E-5	11.178361	15
CGCTTAC	85	5.316538E-5	11.178361	18
GAACCGC	255	0.0	11.175938	6
AGAACCG	265	0.0	11.1118	5
TCGCGTA	190	0.0	11.001643	9
CGTTATT	225	0.0	10.971415	2
CGGAATT	150	2.1282176E-9	10.768488	15
GTCGCGT	195	1.8189894E-12	10.719339	8
GTAGGAC	665	0.0	10.710608	3
>>END_MODULE