FastQCFastQC Report
Thu 2 Feb 2017
SRR4063979_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063979_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2539119
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27500.027.9201491
GGTATCA12250.024.2450281
CGTACAC1401.8553692E-1020.4270733
TAGGACG6400.018.5611384
GATATAC5850.018.0513781
TATACCG1154.2796164E-617.2161275
TAGTACG1154.2796164E-617.2161274
TCAACGC43750.017.1964534
CTAGACG902.2178242E-417.1098564
CAACGCA44950.016.737375
CGCAATA7500.016.72140736
TATCAAC45700.016.7036972
ATCAACG45400.016.6199253
GTGTACG1602.1320375E-816.500091
AACGCAG45900.016.3430276
CGTCTTA2301.8189894E-1216.26063715
CGAATGC8050.016.12399343
GTCCTAC8750.016.0915151
TAAACGC5350.016.03810128
TACGAAT8050.015.85070541
Produced by FastQC (version 0.11.5)