##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063979_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2539119
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32726981287604	32.0	32.0	32.0	32.0	32.0
2	31.368016229251168	32.0	32.0	32.0	32.0	32.0
3	31.45088079763099	32.0	32.0	32.0	32.0	32.0
4	31.535394363162972	32.0	32.0	32.0	32.0	32.0
5	31.479879438498156	32.0	32.0	32.0	32.0	32.0
6	35.05544600312155	36.0	36.0	36.0	36.0	36.0
7	35.07246883663192	36.0	36.0	36.0	36.0	36.0
8	35.013926877787135	36.0	36.0	36.0	36.0	36.0
9	35.1350818925777	36.0	36.0	36.0	36.0	36.0
10	34.97858981796442	36.0	36.0	36.0	36.0	36.0
11	35.12980998527442	36.0	36.0	36.0	36.0	36.0
12	35.03341631487142	36.0	36.0	36.0	36.0	36.0
13	35.075738080806765	36.0	36.0	36.0	36.0	36.0
14	35.018034995602804	36.0	36.0	36.0	36.0	36.0
15	34.990552628687354	36.0	36.0	36.0	36.0	36.0
16	35.005663381668995	36.0	36.0	36.0	36.0	36.0
17	34.971304613923174	36.0	36.0	36.0	36.0	36.0
18	34.968193298541735	36.0	36.0	36.0	36.0	36.0
19	34.96315099843686	36.0	36.0	36.0	36.0	36.0
20	34.95395016933038	36.0	36.0	36.0	36.0	36.0
21	34.955874458818194	36.0	36.0	36.0	36.0	36.0
22	34.938174224996935	36.0	36.0	36.0	36.0	36.0
23	34.879857541139266	36.0	36.0	36.0	32.0	36.0
24	34.85376148183681	36.0	36.0	36.0	32.0	36.0
25	34.82746417162803	36.0	36.0	36.0	32.0	36.0
26	34.759102665137	36.0	36.0	36.0	32.0	36.0
27	34.75778961127856	36.0	36.0	36.0	32.0	36.0
28	34.740003520906264	36.0	36.0	36.0	32.0	36.0
29	34.705322988012774	36.0	36.0	36.0	32.0	36.0
30	34.69328377283617	36.0	36.0	36.0	32.0	36.0
31	34.68367768505533	36.0	36.0	36.0	32.0	36.0
32	34.64848555739215	36.0	36.0	36.0	32.0	36.0
33	34.62586353770737	36.0	36.0	36.0	32.0	36.0
34	34.62608251129624	36.0	36.0	36.0	32.0	36.0
35	34.58294077591479	36.0	36.0	36.0	32.0	36.0
36	34.55886234556159	36.0	36.0	36.0	32.0	36.0
37	34.55421860889545	36.0	36.0	36.0	32.0	36.0
38	34.51872401411671	36.0	36.0	36.0	32.0	36.0
39	34.509097840628975	36.0	36.0	36.0	32.0	36.0
40	34.49182767723766	36.0	36.0	36.0	32.0	36.0
41	34.48229090483746	36.0	36.0	36.0	32.0	36.0
42	34.4213008527761	36.0	36.0	36.0	32.0	36.0
43	34.42006184034699	36.0	36.0	36.0	32.0	36.0
44	34.35920844985997	36.0	36.0	36.0	32.0	36.0
45	34.33706809330323	36.0	36.0	36.0	32.0	36.0
46	34.31694772872008	36.0	36.0	36.0	32.0	36.0
47	34.30371006636554	36.0	36.0	36.0	32.0	36.0
48	34.255178272463795	36.0	36.0	36.0	32.0	36.0
49	34.24228797468729	36.0	36.0	36.0	32.0	36.0
50	33.62464421714776	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	6.0
19	6.0
20	23.0
21	69.0
22	236.0
23	808.0
24	2035.0
25	4740.0
26	9834.0
27	17628.0
28	29622.0
29	45375.0
30	65703.0
31	93431.0
32	135088.0
33	216947.0
34	451791.0
35	1465776.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.34387994118688	18.02879133353211	11.904340835696244	26.722987889584775
2	16.062365331378835	20.16054488533943	36.78133577093958	26.995754012342154
3	18.216275440960903	23.526986184944825	28.823321835884485	29.433416538209784
4	12.302495471854607	16.000943634386573	35.7799299678353	35.91663092592352
5	14.38089353039381	36.64223693336153	33.49378268604189	15.483086850202769
6	34.76085002745048	35.77754165028959	16.29884865532047	13.162759666939463
7	30.514714569952694	30.28955746803218	20.417302714803103	18.77842524721202
8	28.209705807408003	33.374528724333125	18.86000616749353	19.555759300765345
9	27.318874855410623	13.878098702407598	18.13124166284968	40.6717847793321
10	15.757713482727887	26.795946426538926	31.51637218487074	25.92996790586245
11	37.687205680395444	20.757672247736323	22.333139959174815	19.22198211269342
12	24.591816761496222	23.445315832236165	28.85881194131661	23.104055464951003
13	29.7042812773965	19.394140133007653	25.04995860741317	25.851619982182676
14	23.632634349907605	19.365676873368525	24.865819442672173	32.13586933405169
15	25.32817754237655	27.48676458888501	21.651407510450873	25.533650358287563
16	25.99814028373079	25.71764686421984	23.493037091532933	24.791175760516435
17	24.464272844242434	25.756689623448132	24.89410697174886	24.884930560560573
18	25.061881797397913	24.939004338125688	25.856174296949924	24.14293956752648
19	25.846809797732913	24.73122556730329	24.883680081036015	24.53828455392778
20	26.082329764885753	24.238688735326527	24.563881933247373	25.11509956654035
21	26.86258501472361	24.16798897570378	24.1382936364936	24.831132373079008
22	26.212597696441474	24.222928067456575	24.615545180111965	24.94892905598998
23	24.881779116483262	24.131159704824892	25.11379018495902	25.873270993732827
24	25.03086717563866	24.729107303557747	25.014995441307075	25.225030079496513
25	25.24087540644771	24.30978719784236	25.009688579134426	25.439648816575506
26	24.805779911075263	25.144535193172434	25.31030905462424	24.739375841128066
27	25.41171336486589	24.646597843292714	24.816627890993292	25.1250609008481
28	24.999094140110532	24.518318850077826	25.3043689228617	25.178218086949943
29	24.897422425832612	24.522440247430737	25.061026665836962	25.519110660899692
30	24.84312820399714	24.798898470903687	25.301455366961058	25.056517958138112
31	25.46274433038498	24.498479476976314	24.4717753036812	25.56700088895751
32	25.23289176484952	24.708365907788526	24.436217411596047	25.622524915765904
33	24.88826309570697	24.43410791650256	24.971287908625442	25.706341079165025
34	25.464869299386738	24.526288800483016	25.06968165614894	24.939160243981302
35	25.71931459377113	24.337222322215915	25.257015520377905	24.686447563635046
36	24.81088490033203	24.842669943637674	24.88418349462175	25.462261661408547
37	25.726797882957715	24.568119750422802	24.590963263692124	25.114119102927358
38	25.132792160477052	24.39358312359931	24.96035731587716	25.513267400046473
39	25.753278553257758	24.339060914115436	24.411057734912276	25.496602797714534
40	25.931448538354413	24.437822560825754	24.87987661556106	24.750852285258773
41	24.90571180107002	24.77751293410381	25.446037532532184	24.870737732293986
42	25.94547077897143	24.86901746656957	24.876500494864477	24.309011259594524
43	25.267989712849882	24.0151786663726	25.1558235430804	25.561008077697128
44	24.824326825752532	24.710270214919518	24.81196019046194	25.653442768866014
45	25.029488969451595	24.876284173235636	24.79523135302678	25.298995504285994
46	24.907171393823294	24.579179610946923	24.89070878992772	25.622940205302065
47	25.285950704863268	24.368214553809775	25.177958578599913	25.167876162727044
48	25.552590795503104	24.927404950996127	24.361334890324667	25.158669363176106
49	25.064254899165295	24.867807803218927	24.727443438753642	25.340493858862136
50	24.96357937097763	25.055738626897977	24.575328967190124	25.405353034934265
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	14.0
2	23.0
3	86.0
4	149.0
5	204.0
6	259.0
7	248.0
8	237.0
9	336.0
10	435.0
11	652.5
12	870.0
13	1621.5
14	2373.0
15	3039.5
16	3706.0
17	3853.5
18	4001.0
19	4047.0
20	4093.0
21	4994.0
22	5895.0
23	6954.0
24	8013.0
25	10809.0
26	13605.0
27	17898.5
28	22192.0
29	26264.0
30	30336.0
31	34641.0
32	38946.0
33	45811.5
34	52677.0
35	62151.5
36	71626.0
37	82944.5
38	94263.0
39	101224.5
40	108186.0
41	118634.0
42	129082.0
43	132886.5
44	136691.0
45	149224.5
46	161758.0
47	171703.5
48	181649.0
49	189191.0
50	196733.0
51	193279.0
52	189825.0
53	189498.0
54	189171.0
55	187880.0
56	186589.0
57	181481.5
58	176374.0
59	161365.0
60	146356.0
61	129082.5
62	111809.0
63	97877.5
64	83946.0
65	71305.0
66	58664.0
67	51133.0
68	43602.0
69	38158.0
70	32714.0
71	25435.0
72	18156.0
73	15419.0
74	12682.0
75	10089.0
76	7496.0
77	6441.5
78	5387.0
79	4498.0
80	3609.0
81	2917.5
82	2226.0
83	1802.0
84	1378.0
85	1014.5
86	651.0
87	467.5
88	284.0
89	216.0
90	148.0
91	115.0
92	82.0
93	63.5
94	45.0
95	56.0
96	67.0
97	46.0
98	25.0
99	27.5
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0090582599712735
2	6.301398240885914E-4
3	1.9691869502768482E-4
4	0.0
5	0.0
6	3.938373900553696E-5
7	1.1815121701661089E-4
8	0.0
9	0.0012602796481771827
10	0.001023977214143961
11	0.0
12	7.876747801107392E-5
13	6.695235630941283E-4
14	1.1815121701661089E-4
15	0.0038202226835370853
16	8.664422581218131E-4
17	0.0
18	1.5753495602214784E-4
19	3.938373900553696E-5
20	5.119886070719805E-4
21	0.0
22	1.5753495602214784E-4
23	6.301398240885914E-4
24	5.513723460775174E-4
25	0.001851035733260237
26	0.004647281202653361
27	0.006143863284863766
28	0.0037414552055260114
29	0.0025599430353599024
30	0.004056525117570307
31	0.007482910411052023
32	0.0025205592963543654
33	0.0046866649416588985
34	0.002835629208398661
35	0.002756861730387587
36	0.007443526672046485
37	0.004135292595581381
38	0.007443526672046485
39	0.004647281202653361
40	0.0024811755573488285
41	0.0037414552055260114
42	0.0015753495602214786
43	0.0011421284311605717
44	0.0011421284311605717
45	0.0013390471261882566
46	0.0012208959091716458
47	0.0017722682552491632
48	0.0013390471261882566
49	5.119886070719805E-4
50	0.0014178146041993304
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2539119.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.865394236148894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.5388190681512	33.05829564581283
2	15.226730616422445	14.272134665325527
3	5.893604869015603	8.286183469755088
4	2.8770055375813652	5.393279949533366
5	1.5302300688347532	3.585741772397498
6	0.9043741391049943	2.5430310339679987
7	0.5979104945948278	1.961491773298216
8	0.4136677831774903	1.5509362993125477
9	0.2993412427874211	1.2625870818934124
>10	1.5370057023837886	12.844034617668154
>50	0.10277845945866275	3.328824517703868
>100	0.06541235752776471	6.311983031337595
>500	0.009141441184780098	2.8308462829386136
>1k	0.003978219774855882	2.770629859055313
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.876747801107392E-5
2	0.0	0.0	0.0	0.0	7.876747801107392E-5
3	0.0	0.0	0.0	0.0	7.876747801107392E-5
4	0.0	0.0	0.0	0.0	7.876747801107392E-5
5	0.0	0.0	0.0	0.0	1.1815121701661087E-4
6	0.0	0.0	0.0	0.0	1.5753495602214784E-4
7	0.0	0.0	0.0	0.0	1.5753495602214784E-4
8	0.0	0.0	0.0	0.0	1.5753495602214784E-4
9	0.0	0.0	0.0	0.0	1.5753495602214784E-4
10	0.0	0.0	0.0	0.0	1.5753495602214784E-4
11	0.0	0.0	0.0	0.0	1.5753495602214784E-4
12	0.0	0.0	0.0	0.0	2.756861730387587E-4
13	0.0	0.0	0.0	0.0	3.5445365104983266E-4
14	0.0	0.0	0.0	0.0	3.5445365104983266E-4
15	0.0	0.0	0.0	0.0	3.938373900553696E-4
16	0.0	0.0	0.0	0.0	3.938373900553696E-4
17	0.0	0.0	0.0	0.0	3.938373900553696E-4
18	0.0	0.0	0.0	3.938373900553696E-5	4.3322112906090657E-4
19	0.0	0.0	0.0	3.938373900553696E-5	4.3322112906090657E-4
20	0.0	0.0	0.0	7.876747801107392E-5	5.119886070719805E-4
21	0.0	0.0	0.0	1.1815121701661087E-4	5.907560850830544E-4
22	0.0	0.0	0.0	1.969186950276848E-4	5.907560850830544E-4
23	0.0	0.0	0.0	3.938373900553696E-4	5.907560850830544E-4
24	0.0	0.0	0.0	7.089073020996653E-4	5.907560850830544E-4
25	0.0	0.0	0.0	8.270585191162762E-4	5.907560850830544E-4
26	0.0	0.0	0.0	0.001142128431160572	5.907560850830544E-4
27	0.0	0.0	0.0	0.0014571983432048676	6.301398240885914E-4
28	0.0	0.0	0.0	0.0031506991204429567	7.089073020996653E-4
29	0.0	0.0	0.0	0.0072466079770188005	7.089073020996653E-4
30	0.0	0.0	0.0	0.016147332992270152	7.089073020996653E-4
31	0.0	0.0	0.0	0.03816284309636531	7.089073020996653E-4
32	0.0	0.0	0.0	0.058484852423222385	7.089073020996653E-4
33	0.0	0.0	0.0	0.07908254792311821	7.876747801107392E-4
34	0.0	0.0	0.0	0.10137374420025214	8.270585191162762E-4
35	0.0	0.0	0.0	0.12862729159208372	8.270585191162762E-4
36	0.0	0.0	0.0	0.16990145006988644	8.270585191162762E-4
37	0.0	0.0	0.0	0.23393940969288954	8.270585191162762E-4
38	0.0	0.0	0.0	0.3194808908129158	8.664422581218131E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2750	0.0	27.920149	1
GGTATCA	1225	0.0	24.245028	1
CGTACAC	140	1.8553692E-10	20.427073	3
TAGGACG	640	0.0	18.561138	4
GATATAC	585	0.0	18.051378	1
TATACCG	115	4.2796164E-6	17.216127	5
TAGTACG	115	4.2796164E-6	17.216127	4
TCAACGC	4375	0.0	17.196453	4
CTAGACG	90	2.2178242E-4	17.109856	4
CAACGCA	4495	0.0	16.73737	5
CGCAATA	750	0.0	16.721407	36
TATCAAC	4570	0.0	16.703697	2
ATCAACG	4540	0.0	16.619925	3
GTGTACG	160	2.1320375E-8	16.50009	1
AACGCAG	4590	0.0	16.343027	6
CGTCTTA	230	1.8189894E-12	16.260637	15
CGAATGC	805	0.0	16.123993	43
GTCCTAC	875	0.0	16.091515	1
TAAACGC	535	0.0	16.038101	28
TACGAAT	805	0.0	15.850705	41
>>END_MODULE