FastQCFastQC Report
Thu 2 Feb 2017
SRR4063978_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063978_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569464
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24810.15807944623132483No Hit
TATCAACGCAGAGTACTTTTTTTTT18090.11526228062574229No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATT402.7606424E-416.62539113
GGACCGT456.7644444E-414.7771836
TCCAACG1700.013.97403518
CGAATTG551.9587195E-413.81938814
GTATCAA17150.013.6794841
CGACCCG1351.8189894E-1213.368985
GTGCGAA500.001498932613.30073611
TGCGATG655.4513504E-513.15415612
GGTATCA5350.012.7824941
CCAACGA1457.2759576E-1212.45134719
CGAACGA853.932517E-612.29715216
TTTAACC550.003076728712.086573
GATATAC3150.012.0610841
TAAGAGG3750.011.9035314
TAATACC802.8741366E-511.8718734
CCCGACC1701.8189894E-1211.7310823
CGACCAT2350.011.72330710
CGCATCG1302.6138878E-911.69258213
GAACCGG658.026207E-411.6918376
GTTTTAC907.477174E-611.6087921
Produced by FastQC (version 0.11.5)