##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063978_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1569464
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.275752103902988	32.0	32.0	32.0	32.0	32.0
2	30.86591664415367	32.0	32.0	32.0	32.0	32.0
3	30.882291024196796	32.0	32.0	32.0	32.0	32.0
4	30.919881564661566	32.0	32.0	32.0	32.0	32.0
5	30.816160166783053	32.0	32.0	32.0	32.0	32.0
6	34.47977334937278	36.0	36.0	36.0	32.0	36.0
7	34.434444498249086	36.0	36.0	36.0	32.0	36.0
8	34.39091562469735	36.0	36.0	36.0	32.0	36.0
9	34.53355986502398	36.0	36.0	36.0	32.0	36.0
10	34.22271552581009	36.0	36.0	36.0	32.0	36.0
11	34.50509728161971	36.0	36.0	36.0	32.0	36.0
12	34.31547139660419	36.0	36.0	36.0	32.0	36.0
13	34.39953194211527	36.0	36.0	36.0	32.0	36.0
14	34.305422105890926	36.0	36.0	36.0	32.0	36.0
15	34.231234994877234	36.0	36.0	36.0	32.0	36.0
16	34.23104257249609	36.0	36.0	36.0	32.0	36.0
17	34.17068183787586	36.0	36.0	36.0	32.0	36.0
18	34.15679493126316	36.0	36.0	36.0	32.0	36.0
19	34.178093285350926	36.0	36.0	36.0	32.0	36.0
20	34.1513236366046	36.0	36.0	36.0	32.0	36.0
21	34.13415917791042	36.0	36.0	36.0	32.0	36.0
22	34.11497747001524	36.0	36.0	36.0	32.0	36.0
23	34.0544319589363	36.0	36.0	36.0	32.0	36.0
24	34.03643218321669	36.0	36.0	36.0	32.0	36.0
25	33.63354814127626	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	10.0
5	23.0
6	66.0
7	23.0
8	88.0
9	85.0
10	168.0
11	46.0
12	90.0
13	90.0
14	103.0
15	249.0
16	375.0
17	532.0
18	690.0
19	936.0
20	1399.0
21	2071.0
22	3260.0
23	5232.0
24	7927.0
25	11728.0
26	16658.0
27	22641.0
28	30876.0
29	42051.0
30	56168.0
31	76689.0
32	107060.0
33	152147.0
34	324506.0
35	705474.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.29043822136269	17.086164815698943	11.27631677076833	26.347080192170036
2	16.48569274853302	19.441957615082146	38.0383578472759	26.03399178910894
3	18.85945741363513	23.321541486613746	28.844445747098018	28.974555352653102
4	12.540715989723514	15.171325748307643	36.15936709893157	36.12859116303727
5	14.432047653973425	36.57950963255896	34.14780862708714	14.840634086380474
6	34.459301440108284	35.71932449560524	16.6202859060052	13.201088158281276
7	30.140556042380307	30.276109296582494	20.969839878913408	18.613494782123794
8	27.677275016094182	34.04132858262848	19.323853170075658	18.95754323120168
9	27.29051296092185	13.909746875870557	18.40684857367068	40.392891589536916
10	15.543596828394248	26.905669783996995	32.346451452080046	25.204281935528705
11	37.38108626051433	20.64696979549578	22.993751012065736	18.978192931924156
12	24.51892240659297	23.184618268388444	29.806918642386343	22.489540682632242
13	29.75503266273668	19.60771562315503	25.138129648685904	25.499122065422387
14	23.162521606235092	19.633795435258442	25.27165995181116	31.932023006695303
15	24.91380387576537	28.26901351341013	21.859260600465664	24.957922010358836
16	25.430071225316453	25.629956076340072	24.43408996284756	24.505882735495916
17	23.836644757688916	26.033131882950077	25.622984088203772	24.50723927115723
18	24.489976802345524	25.029363716011737	26.71779332790057	23.762866153742166
19	25.550948992982182	24.62236034825103	25.27268484098007	24.554005817786717
20	25.75039423954333	24.67706791633003	25.100225798984706	24.47231204514194
21	26.286907589549024	24.366007237251118	24.49646905038976	24.85061612281009
22	25.819891916019706	24.748553300706213	24.972692056305696	24.458862726968388
23	24.386388245181976	24.687502789868983	25.442584815205453	25.483524149743587
24	24.86741481038899	24.884886659350595	25.499780964229256	24.747917566031155
25	24.84431770792816	24.69772193095779	25.494848424175103	24.963111936938947
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	58.0
1	58.0
2	179.5
3	301.0
4	301.0
5	301.0
6	815.0
7	1329.0
8	1329.0
9	1329.0
10	1689.0
11	2049.0
12	2049.0
13	2049.0
14	2861.0
15	3673.0
16	3673.0
17	3673.0
18	6322.0
19	8971.0
20	8971.0
21	8971.0
22	15092.5
23	21214.0
24	21214.0
25	21214.0
26	32673.5
27	44133.0
28	44133.0
29	44133.0
30	58289.5
31	72446.0
32	72446.0
33	72446.0
34	87838.0
35	103230.0
36	103230.0
37	103230.0
38	119566.0
39	135902.0
40	135902.0
41	135902.0
42	153919.5
43	171937.0
44	171937.0
45	171937.0
46	187267.0
47	202597.0
48	202597.0
49	202597.0
50	207936.5
51	213276.0
52	213276.0
53	213276.0
54	198491.5
55	183707.0
56	183707.0
57	183707.0
58	166975.5
59	150244.0
60	150244.0
61	150244.0
62	130520.5
63	110797.0
64	110797.0
65	110797.0
66	91106.0
67	71415.0
68	71415.0
69	71415.0
70	53664.0
71	35913.0
72	35913.0
73	35913.0
74	27277.0
75	18641.0
76	18641.0
77	18641.0
78	14387.5
79	10134.0
80	10134.0
81	10134.0
82	6962.5
83	3791.0
84	3791.0
85	3791.0
86	2805.0
87	1819.0
88	1819.0
89	1819.0
90	1306.0
91	793.0
92	793.0
93	793.0
94	549.0
95	305.0
96	305.0
97	305.0
98	547.0
99	789.0
100	789.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04109683305892967
2	0.00541586172094422
3	0.0011468883644352467
4	0.0035680971337985453
5	0.00962111905720679
6	0.013189216191005337
7	0.021599730863530477
8	0.03657299562143509
9	0.05014450793391884
10	0.05868245464693679
11	0.05670725801929831
12	0.064098316367881
13	0.06435318044886662
14	0.06696553727896913
15	0.06040278719358966
16	0.06760269748143315
17	0.0640346003476346
18	0.07658665633617592
19	0.07454774368829103
20	0.07996360540923525
21	0.07575834807297267
22	0.07894414908529282
23	0.08219366611785935
24	0.07817955684233599
25	0.07684152041716154
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1569464.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.03487729566739
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.14953200463397	37.10062735383023
2	14.249768163749838	14.259708031298612
3	4.847453177992463	7.276251748720375
4	2.182543985480777	4.368132820237101
5	1.138538539827916	2.8483318068339045
6	0.6932380831910928	2.0811649457489994
7	0.473667022218614	1.6589909934998759
8	0.36049941215873343	1.443003508201796
9	0.27984745323371796	1.2601919685648686
>10	1.4448769794458967	13.050418626561417
>50	0.0998815095189284	3.434944977514751
>100	0.06992688440291382	7.066012958951892
>500	0.008181427316138433	2.8452849954164505
>1k	0.0020453568290346082	1.306935264619736
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2481	0.15807944623132483	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1809	0.11526228062574229	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.371602024640259E-5
2	0.0	0.0	0.0	0.0	6.371602024640259E-5
3	0.0	0.0	0.0	0.0	6.371602024640259E-5
4	0.0	0.0	0.0	0.0	6.371602024640259E-5
5	0.0	0.0	0.0	0.0	6.371602024640259E-5
6	0.0	0.0	0.0	0.0	6.371602024640259E-5
7	0.0	0.0	0.0	0.0	6.371602024640259E-5
8	0.0	0.0	0.0	0.0	6.371602024640259E-5
9	0.0	0.0	0.0	0.0	6.371602024640259E-5
10	0.0	0.0	0.0	0.0	6.371602024640259E-5
11	0.0	0.0	0.0	0.0	6.371602024640259E-5
12	0.0	0.0	0.0	0.0	6.371602024640259E-5
13	0.0	0.0	0.0	0.0	6.371602024640259E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATT	40	2.7606424E-4	16.625391	13
GGACCGT	45	6.7644444E-4	14.777183	6
TCCAACG	170	0.0	13.974035	18
CGAATTG	55	1.9587195E-4	13.819388	14
GTATCAA	1715	0.0	13.679484	1
CGACCCG	135	1.8189894E-12	13.36898	5
GTGCGAA	50	0.0014989326	13.300736	11
TGCGATG	65	5.4513504E-5	13.154156	12
GGTATCA	535	0.0	12.782494	1
CCAACGA	145	7.2759576E-12	12.451347	19
CGAACGA	85	3.932517E-6	12.297152	16
TTTAACC	55	0.0030767287	12.08657	3
GATATAC	315	0.0	12.061084	1
TAAGAGG	375	0.0	11.903531	4
TAATACC	80	2.8741366E-5	11.871873	4
CCCGACC	170	1.8189894E-12	11.731082	3
CGACCAT	235	0.0	11.723307	10
CGCATCG	130	2.6138878E-9	11.692582	13
GAACCGG	65	8.026207E-4	11.691837	6
GTTTTAC	90	7.477174E-6	11.608792	1
>>END_MODULE