FastQCFastQC Report
Thu 2 Feb 2017
SRR4063978_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063978_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569464
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA655.9302147E-727.0784329
GTATCAA18000.027.0117571
AGTATCG752.065637E-623.4657338
GTCCTAA6600.021.3338411
TATCGAG901.0017893E-519.55664610
AATCGGT901.00289235E-519.55415319
CTAAGAC1705.456968E-1219.4103743
ATACGTA708.12349E-418.85759425
CGGGATT2100.018.85579144
GGTATCA7550.018.6494541
AACGCAG26400.018.332026
TCAACGC26250.018.2691674
ATCAACG26600.018.1941853
TATCAAC27100.018.1020222
TTAGGAC6200.018.0954763
CAACGCA26900.017.9094965
TAGGACG8050.017.2161564
TTCTCGC5300.017.01764942
ATTTCGT4700.016.84985542
TCCTAAA7850.016.8140812
Produced by FastQC (version 0.11.5)