##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063978_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1569464
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.355716346472427	32.0	32.0	32.0	32.0	32.0
2	31.409553197779623	32.0	32.0	32.0	32.0	32.0
3	31.48273933011525	32.0	32.0	32.0	32.0	32.0
4	31.564630982297142	32.0	32.0	32.0	32.0	32.0
5	31.522091618539832	32.0	32.0	32.0	32.0	32.0
6	35.1118114209692	36.0	36.0	36.0	36.0	36.0
7	35.13570301708099	36.0	36.0	36.0	36.0	36.0
8	35.07097900939429	36.0	36.0	36.0	36.0	36.0
9	35.18371813561828	36.0	36.0	36.0	36.0	36.0
10	35.039182803810725	36.0	36.0	36.0	36.0	36.0
11	35.17104310771066	36.0	36.0	36.0	36.0	36.0
12	35.090270308844296	36.0	36.0	36.0	36.0	36.0
13	35.12533769490731	36.0	36.0	36.0	36.0	36.0
14	35.07812603538533	36.0	36.0	36.0	36.0	36.0
15	35.0487427554885	36.0	36.0	36.0	36.0	36.0
16	35.07075090604181	36.0	36.0	36.0	36.0	36.0
17	35.02782223740079	36.0	36.0	36.0	36.0	36.0
18	35.0275896739269	36.0	36.0	36.0	36.0	36.0
19	35.024559340003975	36.0	36.0	36.0	36.0	36.0
20	35.012101583725396	36.0	36.0	36.0	36.0	36.0
21	35.00718525560319	36.0	36.0	36.0	36.0	36.0
22	34.99454718298731	36.0	36.0	36.0	36.0	36.0
23	34.94498822527946	36.0	36.0	36.0	36.0	36.0
24	34.92335408776499	36.0	36.0	36.0	36.0	36.0
25	34.888705315955	36.0	36.0	36.0	32.0	36.0
26	34.82792787856236	36.0	36.0	36.0	32.0	36.0
27	34.819646070250734	36.0	36.0	36.0	32.0	36.0
28	34.79816548834506	36.0	36.0	36.0	32.0	36.0
29	34.76672800395549	36.0	36.0	36.0	32.0	36.0
30	34.747798611500485	36.0	36.0	36.0	32.0	36.0
31	34.73856106288516	36.0	36.0	36.0	32.0	36.0
32	34.705326149564435	36.0	36.0	36.0	32.0	36.0
33	34.69474546724232	36.0	36.0	36.0	32.0	36.0
34	34.68605969936233	36.0	36.0	36.0	32.0	36.0
35	34.664293669685954	36.0	36.0	36.0	32.0	36.0
36	34.62180209294383	36.0	36.0	36.0	32.0	36.0
37	34.60426425837101	36.0	36.0	36.0	32.0	36.0
38	34.575513678555225	36.0	36.0	36.0	32.0	36.0
39	34.55520419710169	36.0	36.0	36.0	32.0	36.0
40	34.5482527792928	36.0	36.0	36.0	32.0	36.0
41	34.542683361963064	36.0	36.0	36.0	32.0	36.0
42	34.48160773359567	36.0	36.0	36.0	32.0	36.0
43	34.48030792678264	36.0	36.0	36.0	32.0	36.0
44	34.42419195343123	36.0	36.0	36.0	32.0	36.0
45	34.39457164993909	36.0	36.0	36.0	32.0	36.0
46	34.36249573102664	36.0	36.0	36.0	32.0	36.0
47	34.34422325074038	36.0	36.0	36.0	32.0	36.0
48	34.29769398979524	36.0	36.0	36.0	32.0	36.0
49	34.291617392944346	36.0	36.0	36.0	32.0	36.0
50	33.71071079043546	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	11.0
21	44.0
22	131.0
23	435.0
24	1126.0
25	2663.0
26	5610.0
27	10314.0
28	16962.0
29	26756.0
30	38689.0
31	54915.0
32	79458.0
33	128556.0
34	269446.0
35	934345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.37085453392679	18.024641010364263	11.782060554311222	26.822443901397726
2	15.841707806291621	20.144419835713357	37.46046390714721	26.553408450847808
3	18.129623010221994	23.953922976006382	29.073075499264394	28.84337851450723
4	12.161604216471355	15.893132942201923	36.1487106426143	35.796552198712426
5	14.106663166533288	37.04901800869596	33.84919947192163	14.995119352849127
6	34.574608735100774	35.99522383799279	16.39460935951897	13.035558067387464
7	30.069902890351667	30.433275585343523	20.9715679821697	18.52525354213511
8	27.686458561649076	34.2357008507363	19.103719486397903	18.97412110121672
9	27.28311309652381	13.738469664792758	18.31097195381558	40.66744528486785
10	15.385399589282608	27.175078642249606	32.29400891650509	25.145512851962692
11	37.528098764928664	20.59174342323239	22.90329692175163	18.976860890087316
12	24.47005691762968	23.230969103405563	29.682801930089376	22.616172048875377
13	29.84174801635468	19.61344641688633	25.176892164752314	25.367913402006682
14	23.21504006789592	19.598894142638777	25.133278240109185	32.05278754935612
15	24.84685093405956	28.383393250608833	21.830384621756536	24.939371193575067
16	25.449773679542464	25.586381523279407	24.39373897707231	24.57010582010582
17	23.95123430674421	26.170654439987157	25.42766192789385	24.45044932537478
18	24.585701715429693	25.034773084517553	26.622197047266543	23.75732815278621
19	25.600204910721114	24.764059577027574	25.2063761895781	24.429359322673218
20	25.920779071004723	24.600961222943702	25.024610391223984	24.453649314827594
21	26.330581650805623	24.418655031271822	24.44790068456492	24.80286263335763
22	25.873230283027283	24.706459546595642	25.00535215934143	24.414958011035644
23	24.49850425784747	24.64989439009083	25.422837864099325	25.42876348796238
24	24.98016830024652	24.868027604451733	25.416497552975326	24.73530654232642
25	25.004093829945173	24.552657485018496	25.484011762194676	24.95923692284166
26	24.326106737148493	25.47929511376409	25.843258646045463	24.35133950304195
27	24.853477729506952	25.0620943477258	25.28358224171746	24.800845681049786
28	24.5405250414171	24.96482732254365	25.482286224034663	25.012361412004587
29	24.707233914033594	24.604712420520354	25.318348671202926	25.36970499424312
30	24.382552623446465	25.128822706694574	25.584409378076405	24.904215291782556
31	25.242297766591264	24.516137254277247	24.9556822888457	25.28588269028579
32	25.046769108430418	25.10328709096146	24.693643224341347	25.156300576266784
33	24.38310583575571	24.983018923227693	25.474609944985133	25.159265296031464
34	25.176657843863136	24.958806385551842	25.517293671921692	24.347242098663326
35	25.181659929553412	24.789412112531032	25.663431492988476	24.36549646492708
36	24.323542455739695	25.326185584878345	25.34714965157594	25.003122307806024
37	25.24859787358497	24.81480207110734	25.035204491902014	24.90139556340568
38	24.78533846588132	25.045066974398644	25.046532567062012	25.12306199265802
39	25.54561974961259	24.55384450697333	24.732064574667646	25.168471168746432
40	25.69598605351759	24.763353272657298	25.224604123285683	24.316056550539432
41	24.753713021908247	24.911288262014555	25.868655015996467	24.46634370008073
42	25.65911961066417	25.349073110060772	25.17971355424412	23.812093725030934
43	24.668783343878488	24.71147370305733	25.577578258755825	25.042164694308354
44	24.441571532429535	25.2231197063428	25.01878047646021	25.316528284767458
45	24.561770161184985	25.384293368522126	25.11043755125239	24.943498919040497
46	24.647505474823458	24.773663757592594	25.14786961350327	25.430961154080677
47	25.159564605426056	24.777896646355643	25.44667576128103	24.61586298693727
48	25.13342372674005	25.206379871495237	24.63400865024123	25.026187751523477
49	24.833111069624717	25.187756020075735	24.949202176294094	25.029930734005458
50	24.684714189502174	25.766749986778798	24.599779922762703	24.948755900956325
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	22.5
2	33.0
3	101.0
4	169.0
5	187.0
6	205.0
7	198.0
8	191.0
9	246.5
10	302.0
11	443.5
12	585.0
13	1089.5
14	1594.0
15	2011.5
16	2429.0
17	2436.5
18	2444.0
19	2506.5
20	2569.0
21	3047.0
22	3525.0
23	4355.0
24	5185.0
25	6779.0
26	8373.0
27	11227.0
28	14081.0
29	17360.5
30	20640.0
31	24242.5
32	27845.0
33	33322.0
34	38799.0
35	46184.5
36	53570.0
37	59479.0
38	65388.0
39	68518.5
40	71649.0
41	76417.0
42	81185.0
43	83943.0
44	86701.0
45	94068.0
46	101435.0
47	106535.5
48	111636.0
49	116721.5
50	121807.0
51	119048.0
52	116289.0
53	115759.5
54	115230.0
55	113651.5
56	112073.0
57	107492.0
58	102911.0
59	94416.0
60	85921.0
61	75873.0
62	65825.0
63	56715.5
64	47606.0
65	40470.0
66	33334.0
67	28429.5
68	23525.0
69	20366.5
70	17208.0
71	13406.5
72	9605.0
73	8185.0
74	6765.0
75	5348.5
76	3932.0
77	3366.5
78	2801.0
79	2324.5
80	1848.0
81	1423.5
82	999.0
83	831.5
84	664.0
85	473.5
86	283.0
87	203.0
88	123.0
89	94.0
90	65.0
91	51.5
92	38.0
93	29.0
94	20.0
95	19.0
96	18.0
97	15.0
98	12.0
99	14.5
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008219366611785935
2	7.645922429568311E-4
3	3.1858010123201295E-4
4	0.0
5	0.0
6	1.9114806073920778E-4
7	6.371602024640259E-5
8	0.0
9	0.0010831723441888442
10	9.557403036960389E-4
11	0.0
12	1.9114806073920778E-4
13	3.1858010123201295E-4
14	1.9114806073920778E-4
15	0.004969849579219402
16	5.097281619712207E-4
17	0.0
18	1.9114806073920778E-4
19	0.0
20	3.1858010123201295E-4
21	0.0
22	2.5486408098561035E-4
23	5.734441822176234E-4
24	4.4601214172481816E-4
25	0.0018477645871456754
26	0.004842417538726597
27	0.004969849579219402
28	0.004077825295769766
29	0.0023574927491168958
30	0.0037592451945377527
31	0.007263626308089896
32	0.003313233052812935
33	0.004460121417248181
34	0.0029309369313345194
35	0.0030583689718273245
36	0.0071361942675970905
37	0.003950393255276961
38	0.008474230692771544
39	0.0035680971337985453
40	0.0022937767288704934
41	0.0035043811135521425
42	0.001401752445420857
43	0.0014654684656672597
44	9.557403036960389E-4
45	0.0015929005061600648
46	4.4601214172481816E-4
47	0.0023574927491168958
48	0.0017840485668992726
49	4.4601214172481816E-4
50	9.557403036960389E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1569464.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.52404118953553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.32233523338992	40.31555773523966
2	13.941641131664461	14.924259483618629
3	4.619661099787946	7.417887929602351
4	1.9827824197594885	4.245061116203752
5	1.0479318242405837	2.8044773062239052
6	0.6488923611112265	2.0838804878215353
7	0.4482590048909371	1.679484340895391
8	0.3037660364840744	1.3007028679004444
9	0.24490400209537747	1.179742670607157
>10	1.298125967784935	12.379005132425482
>50	0.07919037582441034	2.9371061121943582
>100	0.05556510848877315	5.929023772711917
>500	0.0056281969045139674	2.0268583075090034
>1k	0.0013172375733968861	0.7769527370465845
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.371602024640259E-5
7	0.0	0.0	0.0	0.0	6.371602024640259E-5
8	0.0	0.0	0.0	0.0	6.371602024640259E-5
9	0.0	0.0	0.0	0.0	1.9114806073920778E-4
10	0.0	0.0	0.0	0.0	1.9114806073920778E-4
11	0.0	0.0	0.0	0.0	1.9114806073920778E-4
12	0.0	0.0	0.0	0.0	2.5486408098561035E-4
13	0.0	0.0	0.0	0.0	2.5486408098561035E-4
14	0.0	0.0	0.0	0.0	3.1858010123201295E-4
15	0.0	0.0	0.0	0.0	3.8229612147841556E-4
16	0.0	0.0	0.0	0.0	3.8229612147841556E-4
17	0.0	0.0	0.0	0.0	3.8229612147841556E-4
18	0.0	0.0	0.0	0.0	3.8229612147841556E-4
19	0.0	0.0	0.0	0.0	3.8229612147841556E-4
20	0.0	0.0	0.0	1.9114806073920778E-4	3.8229612147841556E-4
21	0.0	0.0	0.0	1.9114806073920778E-4	4.4601214172481816E-4
22	0.0	0.0	0.0	2.5486408098561035E-4	4.4601214172481816E-4
23	0.0	0.0	0.0	3.1858010123201295E-4	4.4601214172481816E-4
24	0.0	0.0	0.0	5.097281619712207E-4	4.4601214172481816E-4
25	0.0	0.0	0.0	5.734441822176234E-4	4.4601214172481816E-4
26	0.0	0.0	0.0	8.920242834496363E-4	4.4601214172481816E-4
27	0.0	0.0	0.0	0.0011468883644352467	4.4601214172481816E-4
28	0.0	0.0	0.0	0.0018477645871456752	4.4601214172481816E-4
29	0.0	0.0	0.0	0.00516099763995861	4.4601214172481816E-4
30	0.0	0.0	0.0	0.011405167624106064	4.4601214172481816E-4
31	0.0	0.0	0.0	0.026633296462996284	4.4601214172481816E-4
32	0.0	0.0	0.0	0.04256230152459693	5.097281619712207E-4
33	0.0	0.0	0.0	0.05734441822176233	5.097281619712207E-4
34	0.0	0.0	0.0	0.07461145970853744	5.097281619712207E-4
35	0.0	0.0	0.0	0.09531916628861828	5.097281619712207E-4
36	0.0	0.0	0.0	0.12666744824984835	5.097281619712207E-4
37	0.0	0.0	0.0	0.17381730323218628	5.097281619712207E-4
38	0.0	0.0	0.0	0.24008196428844497	5.097281619712207E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCGA	65	5.9302147E-7	27.078432	9
GTATCAA	1800	0.0	27.011757	1
AGTATCG	75	2.065637E-6	23.465733	8
GTCCTAA	660	0.0	21.333841	1
TATCGAG	90	1.0017893E-5	19.556646	10
AATCGGT	90	1.00289235E-5	19.554153	19
CTAAGAC	170	5.456968E-12	19.410374	3
ATACGTA	70	8.12349E-4	18.857594	25
CGGGATT	210	0.0	18.855791	44
GGTATCA	755	0.0	18.649454	1
AACGCAG	2640	0.0	18.33202	6
TCAACGC	2625	0.0	18.269167	4
ATCAACG	2660	0.0	18.194185	3
TATCAAC	2710	0.0	18.102022	2
TTAGGAC	620	0.0	18.095476	3
CAACGCA	2690	0.0	17.909496	5
TAGGACG	805	0.0	17.216156	4
TTCTCGC	530	0.0	17.017649	42
ATTTCGT	470	0.0	16.849855	42
TCCTAAA	785	0.0	16.814081	2
>>END_MODULE