FastQCFastQC Report
Thu 2 Feb 2017
SRR4063977_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063977_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957034
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29150.30458687988096556No Hit
TATCAACGCAGAGTACTTTTTTTTT23410.24460990936581145No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22520.23531034425109243No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14400.1504648737662403No Hit
GTACATGGAAGCAGTGGTATCAACG14180.14816610486147827No Hit
GTACATGGGGTGGTATCAACGCAAA12810.13385104395455125No Hit
TCCATGTACTCTGCGTTGATACCAC12160.12705922673593623No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11780.12308862590043822No Hit
GGTATCAACGCAGAGTACTTTTTTT10800.1128486553246802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGC551.1257864E-515.5478088
GCTCGTA508.697116E-515.2023019
GTACTAA508.7437846E-515.1927661
TTTTCGG1151.8189894E-1214.87337316
GTTTTCG1203.6379788E-1214.25364915
CCGATAA602.562561E-514.2521589
CCGCGCG400.005271841314.2521569
AAGGCGT602.5714216E-514.2469426
CTATACT400.00528832614.2454534
GTGTAGG1101.8735591E-1013.8116061
TATACTG707.2795247E-613.5670975
ATTAGCT852.7092938E-713.4060833
CGATAAC655.4431453E-513.1551510
CGAGCCG2100.012.669909515
CGTCACT1051.9826984E-812.669909515
ACGAACG604.0806452E-412.669909515
TTTCGGA1352.7284841E-1112.66990917
AACCGCG604.0878533E-412.667267
GAACCGC751.4804395E-512.6639486
AATACCC604.1004943E-412.6626245
Produced by FastQC (version 0.11.5)