##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063977_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	957034
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.215368524002283	32.0	32.0	32.0	32.0	32.0
2	30.834656866945167	32.0	32.0	32.0	32.0	32.0
3	30.863485518800793	32.0	32.0	32.0	32.0	32.0
4	30.877244695590754	32.0	32.0	32.0	32.0	32.0
5	30.81527302060324	32.0	32.0	32.0	32.0	32.0
6	34.44734147376164	36.0	36.0	36.0	32.0	36.0
7	34.38007636092344	36.0	36.0	36.0	32.0	36.0
8	34.33717924337066	36.0	36.0	36.0	32.0	36.0
9	34.46505453306779	36.0	36.0	36.0	32.0	36.0
10	34.18874460050531	36.0	36.0	36.0	32.0	36.0
11	34.45479261969794	36.0	36.0	36.0	32.0	36.0
12	34.27179180676966	36.0	36.0	36.0	32.0	36.0
13	34.34358549435025	36.0	36.0	36.0	32.0	36.0
14	34.257415097060296	36.0	36.0	36.0	32.0	36.0
15	34.20494256212423	36.0	36.0	36.0	32.0	36.0
16	34.1967777529325	36.0	36.0	36.0	32.0	36.0
17	34.128784348309466	36.0	36.0	36.0	32.0	36.0
18	34.10742669539431	36.0	36.0	36.0	32.0	36.0
19	34.130414384441934	36.0	36.0	36.0	32.0	36.0
20	34.09805503252758	36.0	36.0	36.0	32.0	36.0
21	34.07399841593924	36.0	36.0	36.0	32.0	36.0
22	34.06301030057448	36.0	36.0	36.0	32.0	36.0
23	34.015766420001796	36.0	36.0	36.0	32.0	36.0
24	33.99075163473816	36.0	36.0	36.0	32.0	36.0
25	33.57863356996721	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	13.0
5	8.0
6	43.0
7	16.0
8	53.0
9	67.0
10	89.0
11	20.0
12	62.0
13	66.0
14	133.0
15	217.0
16	322.0
17	407.0
18	553.0
19	726.0
20	1108.0
21	1598.0
22	2476.0
23	3742.0
24	5329.0
25	7933.0
26	10932.0
27	14405.0
28	19315.0
29	26032.0
30	33870.0
31	45818.0
32	63686.0
33	89494.0
34	192404.0
35	436091.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.04713251420101	17.039123081007965	11.113166956227225	25.8005774485638
2	16.300737014469643	20.21099952036064	37.6342389356395	25.854024529530218
3	18.392416411881122	24.740285931031313	28.78317349589452	28.084124161193042
4	12.137785463873897	15.549193046985616	36.226939534689315	36.086081954451174
5	14.081492751926966	37.17448460933973	33.91400123727198	14.830021401461318
6	33.59995819834884	35.93489392831017	17.326679903856203	13.138467969484793
7	29.875377296176453	30.87871338868404	21.290164797953196	17.95574451718631
8	28.34905098288445	32.755045083278105	19.797966705481702	19.09793722835574
9	27.716061773155527	13.885473774008606	18.507796916360352	39.89066753647552
10	15.396566505656272	26.953976120276856	32.42163812391527	25.2278192501516
11	36.84700819582148	21.110905492423647	22.782271765870714	19.259814545884158
12	24.4554835377285	23.958306104460586	29.527216151417974	22.05899420639294
13	29.492841817337734	19.76655917891065	25.493732669167745	25.246866334583874
14	23.475315832289446	19.659815007288035	25.84157463387295	31.02329452654957
15	24.944245963909655	27.318170744840465	22.961252905326354	24.776330385923533
16	25.326153443994247	25.854920925369235	24.277087962357864	24.541837668278657
17	23.53259159114886	25.97153692451984	25.84000158929817	24.65586989503313
18	24.39390405995129	25.034849928313204	27.002550606906407	23.568695404829107
19	25.209377519510355	25.31875890815205	25.77855811069271	23.693305461644883
20	25.30285284029114	24.48977244206475	25.661131096795785	24.546243620848323
21	26.423943414536204	24.470556987016465	24.83069953821443	24.274800060232902
22	25.430793781594506	24.5652323954414	25.333016821820053	24.670957001144043
23	23.982007872862887	24.566720630498352	25.882455061514587	25.56881643512418
24	24.335236541598697	25.57472706738612	25.52317312920902	24.566863261806166
25	24.5870283610809	24.93798627959508	25.516920438383668	24.958064920940352
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	204.0
1	204.0
2	391.0
3	578.0
4	578.0
5	578.0
6	1796.5
7	3015.0
8	3015.0
9	3015.0
10	2881.5
11	2748.0
12	2748.0
13	2748.0
14	2679.5
15	2611.0
16	2611.0
17	2611.0
18	4615.5
19	6620.0
20	6620.0
21	6620.0
22	10781.0
23	14942.0
24	14942.0
25	14942.0
26	21948.0
27	28954.0
28	28954.0
29	28954.0
30	35920.5
31	42887.0
32	42887.0
33	42887.0
34	52582.0
35	62277.0
36	62277.0
37	62277.0
38	71168.5
39	80060.0
40	80060.0
41	80060.0
42	91083.5
43	102107.0
44	102107.0
45	102107.0
46	116792.5
47	131478.0
48	131478.0
49	131478.0
50	130932.5
51	130387.0
52	130387.0
53	130387.0
54	119299.0
55	108211.0
56	108211.0
57	108211.0
58	98852.0
59	89493.0
60	89493.0
61	89493.0
62	77283.5
63	65074.0
64	65074.0
65	65074.0
66	53438.5
67	41803.0
68	41803.0
69	41803.0
70	31337.5
71	20872.0
72	20872.0
73	20872.0
74	15851.0
75	10830.0
76	10830.0
77	10830.0
78	8796.0
79	6762.0
80	6762.0
81	6762.0
82	4613.0
83	2464.0
84	2464.0
85	2464.0
86	1930.5
87	1397.0
88	1397.0
89	1397.0
90	981.5
91	566.0
92	566.0
93	566.0
94	382.0
95	198.0
96	198.0
97	198.0
98	347.0
99	496.0
100	496.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.043049672216452076
2	0.006791817218615013
3	0.002089789913420004
4	0.004075090331169008
5	0.009404054610390018
6	0.014001592419914027
7	0.023614626021646046
8	0.03761621844156007
9	0.0524537268268421
10	0.05997697051515411
11	0.05966350202814111
12	0.06635082975108512
13	0.06750021420346612
14	0.07021694109091213
15	0.06279818689827112
16	0.06885857764718913
17	0.06655980874242713
18	0.08265119107576115
19	0.07805365326623714
20	0.08296465956277416
21	0.07794916377056615
22	0.08108384864069615
23	0.08756219737229816
24	0.07878507973593415
25	0.08296465956277416
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	957034.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.35574783839476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.26054948163029	41.451991976409225
2	14.137912405931244	15.369536033962236
3	4.510269118929362	7.354771527354701
4	1.8807726809972982	4.089232223585232
5	0.9330069591117988	2.5357145500474214
6	0.5337818955294672	1.7408468468460054
7	0.3556021766200594	1.3530315570200986
8	0.22528381796775623	0.9796376321220948
9	0.16623617605855684	0.8132302500712121
>10	0.8235792965977653	8.048436790863356
>50	0.07946664780297832	3.0749491975543735
>100	0.08447470559800117	8.95735156524017
>500	0.007328855629105521	2.6460899424380866
>1k	0.0017357815963670968	1.58517990648573
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2915	0.30458687988096556	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2341	0.24460990936581145	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2252	0.23531034425109243	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1440	0.1504648737662403	No Hit
GTACATGGAAGCAGTGGTATCAACG	1418	0.14816610486147827	No Hit
GTACATGGGGTGGTATCAACGCAAA	1281	0.13385104395455125	No Hit
TCCATGTACTCTGCGTTGATACCAC	1216	0.12705922673593623	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1178	0.12308862590043822	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1080	0.1128486553246802	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.089789913420004E-4
7	0.0	0.0	0.0	0.0	2.089789913420004E-4
8	0.0	0.0	0.0	0.0	2.089789913420004E-4
9	0.0	0.0	0.0	0.0	2.089789913420004E-4
10	0.0	0.0	0.0	1.044894956710002E-4	2.089789913420004E-4
11	0.0	0.0	0.0	1.044894956710002E-4	2.089789913420004E-4
12	0.0	0.0	0.0	1.044894956710002E-4	2.089789913420004E-4
13	0.0	0.0	0.0	1.044894956710002E-4	3.134684870130006E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTAGC	55	1.1257864E-5	15.547808	8
GCTCGTA	50	8.697116E-5	15.202301	9
GTACTAA	50	8.7437846E-5	15.192766	1
TTTTCGG	115	1.8189894E-12	14.873373	16
GTTTTCG	120	3.6379788E-12	14.253649	15
CCGATAA	60	2.562561E-5	14.252158	9
CCGCGCG	40	0.0052718413	14.252156	9
AAGGCGT	60	2.5714216E-5	14.246942	6
CTATACT	40	0.005288326	14.245453	4
GTGTAGG	110	1.8735591E-10	13.811606	1
TATACTG	70	7.2795247E-6	13.567097	5
ATTAGCT	85	2.7092938E-7	13.406083	3
CGATAAC	65	5.4431453E-5	13.15515	10
CGAGCCG	210	0.0	12.6699095	15
CGTCACT	105	1.9826984E-8	12.6699095	15
ACGAACG	60	4.0806452E-4	12.6699095	15
TTTCGGA	135	2.7284841E-11	12.669909	17
AACCGCG	60	4.0878533E-4	12.66726	7
GAACCGC	75	1.4804395E-5	12.663948	6
AATACCC	60	4.1004943E-4	12.662624	5
>>END_MODULE