FastQCFastQC Report
Thu 2 Feb 2017
SRR4063977_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063977_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957034
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9660.10093685281818618No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGT450.001398474724.4430242
TCTATAG803.6160654E-621.998723
TAATACC1306.91216E-1121.9987184
TAGAGCG755.473561E-520.5321375
ACGACGG2850.020.0690086
TGTGCGA550.004480384820.0019710
TACGACG2750.019.9988375
CGACGGT2800.019.6417147
TTAGGAC1705.456968E-1219.4106353
GACGGTA2750.019.1988838
TATGGCG1504.656613E-1019.06555616
GGCGTTA1851.8189894E-1219.02591942
GGACCGT1051.7928905E-618.8560476
ACGGTAT2850.018.5281419
GTAATAC951.5973435E-518.5252383
TAGCGTA1701.2732926E-1018.1165927
GTACTAA851.431076E-418.1165921
TTAACGG1102.7925598E-618.00177435
CTACGAC2950.017.8972644
CCCGTCG1002.4833791E-517.59897641
Produced by FastQC (version 0.11.5)