##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063977_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	957034
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266573601355855	32.0	32.0	32.0	32.0	32.0
2	31.397243985062183	32.0	32.0	32.0	32.0	32.0
3	31.480336121809675	32.0	32.0	32.0	32.0	32.0
4	31.564295521371236	32.0	32.0	32.0	32.0	32.0
5	31.508729052468354	32.0	32.0	32.0	32.0	32.0
6	35.086708518192665	36.0	36.0	36.0	36.0	36.0
7	35.11372845687823	36.0	36.0	36.0	36.0	36.0
8	35.05828424068528	36.0	36.0	36.0	36.0	36.0
9	35.16973064697806	36.0	36.0	36.0	36.0	36.0
10	35.02683917185805	36.0	36.0	36.0	36.0	36.0
11	35.17199388945429	36.0	36.0	36.0	36.0	36.0
12	35.07062236033411	36.0	36.0	36.0	36.0	36.0
13	35.11789967754542	36.0	36.0	36.0	36.0	36.0
14	35.06013788433849	36.0	36.0	36.0	36.0	36.0
15	35.030204778513614	36.0	36.0	36.0	36.0	36.0
16	35.04665351492214	36.0	36.0	36.0	36.0	36.0
17	35.006224439257124	36.0	36.0	36.0	36.0	36.0
18	34.99628226374403	36.0	36.0	36.0	36.0	36.0
19	34.99845877993885	36.0	36.0	36.0	36.0	36.0
20	34.99359374902041	36.0	36.0	36.0	36.0	36.0
21	34.98039986040203	36.0	36.0	36.0	36.0	36.0
22	34.96912753360905	36.0	36.0	36.0	36.0	36.0
23	34.90970749210582	36.0	36.0	36.0	32.0	36.0
24	34.88819101515725	36.0	36.0	36.0	32.0	36.0
25	34.85238977925549	36.0	36.0	36.0	32.0	36.0
26	34.79719111337737	36.0	36.0	36.0	32.0	36.0
27	34.786359732256116	36.0	36.0	36.0	32.0	36.0
28	34.75707028172458	36.0	36.0	36.0	32.0	36.0
29	34.73301784471607	36.0	36.0	36.0	32.0	36.0
30	34.70630301535787	36.0	36.0	36.0	32.0	36.0
31	34.70702399287799	36.0	36.0	36.0	32.0	36.0
32	34.652764687565956	36.0	36.0	36.0	32.0	36.0
33	34.61990901054717	36.0	36.0	36.0	32.0	36.0
34	34.61157910795228	36.0	36.0	36.0	32.0	36.0
35	34.55209532785669	36.0	36.0	36.0	32.0	36.0
36	34.52337221039169	36.0	36.0	36.0	32.0	36.0
37	34.49826860905673	36.0	36.0	36.0	32.0	36.0
38	34.45546344226015	36.0	36.0	36.0	32.0	36.0
39	34.433115228926034	36.0	36.0	36.0	32.0	36.0
40	34.393993316851855	36.0	36.0	36.0	32.0	36.0
41	34.375058775341316	36.0	36.0	36.0	32.0	36.0
42	34.29751189612908	36.0	36.0	36.0	32.0	36.0
43	34.282814403668	36.0	36.0	36.0	32.0	36.0
44	34.2148972763768	36.0	36.0	36.0	32.0	36.0
45	34.160796795098186	36.0	36.0	36.0	32.0	36.0
46	34.1513540793744	36.0	36.0	36.0	32.0	36.0
47	34.09289743102126	36.0	36.0	36.0	32.0	36.0
48	34.055150600710114	36.0	36.0	36.0	32.0	36.0
49	34.01926681810678	36.0	36.0	36.0	32.0	36.0
50	33.412544381913285	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	0.0
19	6.0
20	14.0
21	37.0
22	124.0
23	340.0
24	884.0
25	1968.0
26	4172.0
27	7526.0
28	12078.0
29	18407.0
30	25371.0
31	35431.0
32	50974.0
33	80201.0
34	163047.0
35	556451.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.037373137822065	17.992797906669562	11.655749970740189	26.31407898476818
2	15.652745429334805	20.829715358082897	37.11473135031718	26.40280786226512
3	17.715733653770798	25.219299391342965	29.049815835531987	28.015151119354247
4	11.779205336487523	16.158673568546153	36.40142356488902	35.6606975300773
5	13.673390914011415	37.64035551506007	33.688771767774185	14.997481803154328
6	33.67933360639979	36.377675981576374	16.976025880012372	12.966964532011469
7	29.651015168755933	30.920772846913323	21.389544561159333	18.038667423171407
8	28.265975921440617	33.045847900910516	19.608289778628553	19.079886399020307
9	27.646984450739424	13.765395398020738	18.450967218133734	40.1366529331061
10	15.250039967524428	27.303409412691597	32.340257902859584	25.106292716924393
11	37.02522585404489	21.00458290928013	22.819356470094064	19.150834766580914
12	24.34046092502654	24.10285131531652	29.475816900584306	22.080870859072633
13	29.688577554679696	19.579573429408544	25.551812486155058	25.180036529756705
14	23.511623946091724	19.67100403016406	25.77925735058248	31.038114673161743
15	25.074999921630177	27.354098898538247	22.914396118708343	24.656505061123237
16	25.370498661993835	25.894834073651467	24.268537472009495	24.466129792345203
17	23.586204878823533	25.957071535598526	25.8833019516548	24.573421633923143
18	24.467571549481207	24.873932896565414	27.08608925530025	23.572406298653124
19	25.30780025349178	25.124525486581966	25.975279849284195	23.592394410642058
20	25.33745164708169	24.322850162900487	25.97306656245494	24.36663162756289
21	26.499894465609376	24.421075949234826	24.990648190137446	24.08838139501836
22	25.414379650440477	24.54920572226872	25.481670955964947	24.554743671325856
23	24.062748490899423	24.35396284535337	26.035733579094423	25.547555084652785
24	24.42384130871824	25.31148513941076	25.74240252113836	24.522271030732643
25	24.656798513716566	24.560039623079188	25.797633900302813	24.98552796290143
26	24.321279852871257	25.327983197228797	26.30114369608719	24.049593253812755
27	25.007549799943362	24.990726024291025	25.423650884711158	24.57807329105445
28	24.16838559071426	24.73484682244612	26.249171886135127	24.8475957007045
29	24.264532241042414	25.08928851318168	26.122715541112424	24.523463704663484
30	24.43495179718244	25.16598780770702	26.349793834469875	24.04926656064067
31	24.528351177998708	25.120119712172478	25.450853847906185	24.900675261922625
32	24.24018557799814	25.2336966175902	25.36702855769532	25.159089246716338
33	24.027080714447678	24.912251003405466	25.876000923728643	25.18466735841821
34	24.618681746984617	24.97102953054778	26.096410796554682	24.313877925912923
35	25.316244067484906	24.858988478747438	25.912055623010737	23.912711830756916
36	24.353261800960546	25.430322815260055	25.56000484870778	24.656410535071625
37	25.048589544783884	25.42581160213836	25.340022319842507	24.18557653323525
38	24.389525163016216	25.272320682160178	25.687489550242432	24.650664604581173
39	24.68560310389544	25.337228876760598	25.259067222157782	24.718100797186178
40	25.113975371079565	25.37468456277658	25.573116127919914	23.93822393822394
41	24.055799373040752	25.80135841170324	25.939602925809822	24.203239289446184
42	25.418801775518908	25.71984167453836	25.32590886672741	23.535447683215327
43	24.518818833462205	24.881820651606027	25.598838059810664	25.000522455121104
44	24.447163535938156	25.505763208462945	25.318933061935027	24.728140193663865
45	24.47357201969455	25.560283213655783	25.34273199194162	24.62341277470805
46	24.222460228311697	25.521799326036415	25.351688827355606	24.904051618296283
47	24.622681334481346	25.174082773955714	25.733216581540958	24.470019310021986
48	25.254513746302326	25.974142678164846	24.57856201692544	24.192781558607386
49	24.29479597253163	25.90039162908504	25.209920712873608	24.594891685509722
50	24.34086786427813	26.2990378426275	24.799376394961005	24.560717898133362
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	64.0
1	54.5
2	45.0
3	140.0
4	235.0
5	271.0
6	307.0
7	277.5
8	248.0
9	375.0
10	502.0
11	678.5
12	855.0
13	1559.5
14	2264.0
15	2953.0
16	3642.0
17	3917.0
18	4192.0
19	4262.5
20	4333.0
21	4556.0
22	4779.0
23	5250.0
24	5721.0
25	6133.0
26	6545.0
27	8021.5
28	9498.0
29	11009.0
30	12520.0
31	14124.0
32	15728.0
33	18254.0
34	20780.0
35	24136.5
36	27493.0
37	32151.5
38	36810.0
39	39866.0
40	42922.0
41	47349.5
42	51777.0
43	52973.0
44	54169.0
45	59985.0
46	65801.0
47	69709.5
48	73618.0
49	74438.0
50	75258.0
51	71697.5
52	68137.0
53	66848.0
54	65559.0
55	65581.0
56	65603.0
57	63969.5
58	62336.0
59	55823.0
60	49310.0
61	43583.5
62	37857.0
63	32513.0
64	27169.0
65	23114.0
66	19059.0
67	16378.0
68	13697.0
69	12235.5
70	10774.0
71	8425.0
72	6076.0
73	5192.5
74	4309.0
75	3335.0
76	2361.0
77	2115.0
78	1869.0
79	1532.5
80	1196.0
81	937.0
82	678.0
83	604.5
84	531.0
85	358.5
86	186.0
87	136.5
88	87.0
89	66.0
90	45.0
91	38.5
92	32.0
93	25.5
94	19.0
95	18.5
96	18.0
97	14.5
98	11.0
99	10.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009404054610390018
2	7.314264696970014E-4
3	9.404054610390017E-4
4	0.0
5	0.0
6	2.089789913420004E-4
7	1.044894956710002E-4
8	0.0
9	0.0011493844523810023
10	7.314264696970014E-4
11	0.0
12	2.089789913420004E-4
13	6.269369740260012E-4
14	1.044894956710002E-4
15	0.0034481533571430064
16	0.0013583634437230024
17	0.0
18	4.179579826840008E-4
19	1.044894956710002E-4
20	8.359159653680016E-4
21	0.0
22	1.044894956710002E-4
23	7.314264696970014E-4
24	6.269369740260012E-4
25	0.0016718319307360032
26	0.005119985287879009
27	0.0057469222619050105
28	0.004179579826840008
29	0.0025077478961040046
30	0.003761621844156007
31	0.007523243688312014
32	0.0025077478961040046
33	0.004493048313853008
34	0.002612237391775005
35	0.002089789913420004
36	0.008150180662338016
37	0.003970600835498007
38	0.007732222679654014
39	0.004493048313853008
40	0.0030301953744590058
41	0.003552642852814007
42	0.0018808109220780035
43	0.0014628529393940029
44	0.0015673424350650027
45	0.0018808109220780035
46	9.404054610390017E-4
47	0.0018808109220780035
48	0.0013583634437230024
49	6.269369740260012E-4
50	0.0018808109220780035
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	957034.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.835932038029924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.23688394344593	44.67067170582343
2	13.776514351423272	15.935550954997204
3	4.292595058410611	7.447987081950576
4	1.7623859409181293	4.077169340148814
5	0.8741580137954915	2.5278871738187636
6	0.5006447965245693	1.7373155056192988
7	0.32288243831449187	1.3071944731041238
8	0.220478725063877	1.02012740469007
9	0.13986797331096093	0.7280455138838747
>10	0.7250866135892216	7.463077943614481
>50	0.07307448953294635	3.0088138154421435
>100	0.06979330085316587	7.827984123312937
>500	0.0056343548174728995	2.2481749635943147
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	966	0.10093685281818618	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.044894956710002E-4
7	0.0	0.0	0.0	0.0	1.044894956710002E-4
8	0.0	0.0	0.0	0.0	1.044894956710002E-4
9	0.0	0.0	0.0	0.0	1.044894956710002E-4
10	0.0	0.0	0.0	0.0	1.044894956710002E-4
11	0.0	0.0	0.0	0.0	1.044894956710002E-4
12	0.0	0.0	0.0	0.0	3.134684870130006E-4
13	0.0	0.0	0.0	0.0	3.134684870130006E-4
14	0.0	0.0	0.0	0.0	3.134684870130006E-4
15	0.0	0.0	0.0	0.0	5.22447478355001E-4
16	0.0	0.0	0.0	1.044894956710002E-4	5.22447478355001E-4
17	0.0	0.0	0.0	1.044894956710002E-4	7.314264696970013E-4
18	0.0	0.0	0.0	3.134684870130006E-4	7.314264696970013E-4
19	0.0	0.0	0.0	3.134684870130006E-4	7.314264696970013E-4
20	0.0	0.0	0.0	7.314264696970013E-4	0.001149384452381002
21	0.0	0.0	0.0	0.001044894956710002	0.001149384452381002
22	0.0	0.0	0.0	0.0012538739480520023	0.001149384452381002
23	0.0	0.0	0.0	0.0028212163831170052	0.001149384452381002
24	0.0	0.0	0.0	0.003866111339827007	0.001149384452381002
25	0.0	0.0	0.0	0.004597537809524008	0.001149384452381002
26	0.0	0.0	0.0	0.0058514117575760105	0.0012538739480520023
27	0.0	0.0	0.0	0.006896306714286013	0.0012538739480520023
28	0.0	0.0	0.0	0.009822012593074018	0.0012538739480520023
29	0.0	0.0	0.0	0.013897102924243026	0.0012538739480520023
30	0.0	0.0	0.0	0.026122373917750047	0.0012538739480520023
31	0.0	0.0	0.0	0.04712476254762109	0.0012538739480520023
32	0.0	0.0	0.0	0.07053040957792513	0.0012538739480520023
33	0.0	0.0	0.0	0.09195075619048017	0.0012538739480520023
34	0.0	0.0	0.0	0.11546089271645521	0.0012538739480520023
35	0.0	0.0	0.0	0.14367305654762527	0.0012538739480520023
36	0.0	0.0	0.0	0.18473742834632834	0.0012538739480520023
37	0.0	0.0	0.0	0.24502786734849547	0.0012538739480520023
38	0.0	0.0	0.0	0.3191109197792346	0.0012538739480520023
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCCGT	45	0.0013984747	24.443024	2
TCTATAG	80	3.6160654E-6	21.99872	3
TAATACC	130	6.91216E-11	21.998718	4
TAGAGCG	75	5.473561E-5	20.532137	5
ACGACGG	285	0.0	20.069008	6
TGTGCGA	55	0.0044803848	20.00197	10
TACGACG	275	0.0	19.998837	5
CGACGGT	280	0.0	19.641714	7
TTAGGAC	170	5.456968E-12	19.410635	3
GACGGTA	275	0.0	19.198883	8
TATGGCG	150	4.656613E-10	19.065556	16
GGCGTTA	185	1.8189894E-12	19.025919	42
GGACCGT	105	1.7928905E-6	18.856047	6
ACGGTAT	285	0.0	18.528141	9
GTAATAC	95	1.5973435E-5	18.525238	3
TAGCGTA	170	1.2732926E-10	18.116592	7
GTACTAA	85	1.431076E-4	18.116592	1
TTAACGG	110	2.7925598E-6	18.001774	35
CTACGAC	295	0.0	17.897264	4
CCCGTCG	100	2.4833791E-5	17.598976	41
>>END_MODULE