FastQCFastQC Report
Thu 2 Feb 2017
SRR4063976_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063976_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421397
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30440.2141555103887232No Hit
TATCAACGCAGAGTACTTTTTTTTT21020.1478826816153404No Hit
GATTAAGAGGGACGGCCGGGGGCAT18990.13360095736799782No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG18790.13219389093968822No Hit
GCGCAAGACGGACCAGAGCGAAAGC18090.12726915844060455No Hit
GTCTTGCGCCGGTCCAAGAATTTCA17910.1260027986551259No Hit
GAATAGGACCGCGGTTCTATTTTGT17710.12459573222681629No Hit
ATCAGATACCGTCGTAGTTCCGACC17480.12297760583426023No Hit
GTATCTGATCGTCTTCGAACCTCCG17210.12107806615604226No Hit
GTACATGGGGAATAATTGCAATCCC16430.11559050708563477No Hit
GGGTAGGCACACGCTGAGCCAGTCA16270.11446485394298708No Hit
TCGTAGTTCCGACCATAAACGATGC16140.11355026076458584No Hit
GAACTACGACGGTATCTGATCGTCT15930.11207284101486072No Hit
CTATTGGAGCTGGAATTACCGCGGC15660.11017330133664274No Hit
GTCCTATTCCATTATTCCTAGCTGC15090.10616316201596035No Hit
GAATAACGCCGCCGCATCGCCAGTC14750.10377114908783401No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACAA250.006028536719.00179713
CGTTATT2050.015.2871322
TACTAGA508.740128E-515.1944812
CGAACGA1350.014.78021616
GCGTTAT2000.014.7227641
TCGGCAA551.9583944E-413.81948813
CGACCAT3450.013.49402810
ACGCGCG852.6953603E-713.41303313
GTTATTC2350.012.9314743
TAACACA1052.0019797E-812.6625144
CTAGGAC1900.012.4954623
CCGACCA3650.012.4943319
GTCTTAG1301.9826984E-1012.4211891
TCGAACG1405.2750693E-1112.2098513
ACGAACG1650.012.09290315
TTATGTT550.00307576712.0869454
GTCCTAC2150.011.9284191
TAACGAA1850.011.81192813
CGAGATT1302.6266207E-911.6884744
AAGACGG3750.011.6507435
Produced by FastQC (version 0.11.5)