##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063976_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1421397
Sequences flagged as poor quality	0
Sequence length	25
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2484295379827	32.0	32.0	32.0	32.0	32.0
2	30.7790314739654	32.0	32.0	32.0	32.0	32.0
3	30.800487829930695	32.0	32.0	32.0	32.0	32.0
4	30.83862847606967	32.0	32.0	32.0	32.0	32.0
5	30.714845324705202	32.0	32.0	32.0	32.0	32.0
6	34.394678615474774	36.0	36.0	36.0	32.0	36.0
7	34.32794919364541	36.0	36.0	36.0	32.0	36.0
8	34.27245027251359	36.0	36.0	36.0	32.0	36.0
9	34.43723885726507	36.0	36.0	36.0	32.0	36.0
10	34.07414395837335	36.0	36.0	36.0	32.0	36.0
11	34.417357008633054	36.0	36.0	36.0	32.0	36.0
12	34.18305512112379	36.0	36.0	36.0	32.0	36.0
13	34.29979379441493	36.0	36.0	36.0	32.0	36.0
14	34.16403087947984	36.0	36.0	36.0	32.0	36.0
15	34.08608573115041	36.0	36.0	36.0	32.0	36.0
16	34.092487179866005	36.0	36.0	36.0	32.0	36.0
17	34.00159983452899	36.0	36.0	36.0	32.0	36.0
18	34.00011115824783	36.0	36.0	36.0	32.0	36.0
19	34.02843048071721	36.0	36.0	36.0	32.0	36.0
20	34.00388350334214	36.0	36.0	36.0	32.0	36.0
21	33.98651960008358	36.0	36.0	36.0	32.0	36.0
22	33.962270920791305	36.0	36.0	36.0	32.0	36.0
23	33.88956850197376	36.0	36.0	36.0	32.0	36.0
24	33.88103182995321	36.0	36.0	36.0	32.0	36.0
25	33.47025918867143	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	12.0
5	11.0
6	60.0
7	21.0
8	74.0
9	91.0
10	136.0
11	40.0
12	88.0
13	89.0
14	126.0
15	196.0
16	387.0
17	456.0
18	725.0
19	945.0
20	1471.0
21	2314.0
22	3554.0
23	5675.0
24	8476.0
25	12157.0
26	16885.0
27	22381.0
28	30272.0
29	40985.0
30	53267.0
31	72472.0
32	102329.0
33	144010.0
34	301128.0
35	600560.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.92431447869696	16.82031849881726	10.928716763803152	26.326650258682637
2	17.662715398968277	19.368739956491027	36.22037604586173	26.748168598678973
3	19.40619512359854	23.13511695708947	27.75022231978027	29.70846559953172
4	13.160638603245362	15.04694122216031	34.549428994365925	37.2429911802284
5	15.561862934587053	35.692338434195506	32.63899784909619	16.10680078212125
6	35.262437975474455	34.31711806542585	16.440122937323917	13.98032102177578
7	31.273146926468154	29.5280718605854	20.336081233705233	18.862699979241214
8	28.927048957073094	31.6510387744861	19.088127798926983	20.333784469513823
9	27.80657045540451	13.908916282405839	18.175284878576864	40.10922838361279
10	16.91067463898599	25.61091158412538	30.196144960305	27.282268816583628
11	38.137591061811165	20.569603991550057	21.69166430264374	19.60114064399504
12	25.646236905074502	23.18131803535815	27.550082964153994	23.62236209541335
13	29.918838037216656	18.559260763692606	24.769217447745255	26.752683751345486
14	24.38399369209541	19.252133141843373	24.053251105291316	32.3106220607699
15	25.861625957997692	26.414654698456435	21.559391809176926	26.164327534368947
16	26.733312447859646	24.817856375994847	22.869100880357916	25.579730295787588
17	24.948961920665003	25.078070662044844	24.364734306318585	25.608233110971568
18	26.052647142482982	23.74584953855599	25.325559520222146	24.875943798738877
19	26.393527801559053	24.05199556166359	24.497518924886226	25.056957711891137
20	26.72291793107624	23.3223543857821	23.80063564304292	26.154092040098742
21	27.932377992714553	23.245065004372112	23.535694321041195	25.286862681872137
22	27.164703480226148	23.26442818821243	23.875351155733608	25.695517175827813
23	25.13432541568532	23.23035814243317	24.790511496255927	26.844804945625576
24	25.600341332251887	24.11947473697773	24.45376630709905	25.826417623671333
25	25.67795345221868	23.5995955795379	24.343168786400657	26.37928218184276
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	121.0
1	121.0
2	279.5
3	438.0
4	438.0
5	438.0
6	1141.5
7	1845.0
8	1845.0
9	1845.0
10	1921.5
11	1998.0
12	1998.0
13	1998.0
14	2215.5
15	2433.0
16	2433.0
17	2433.0
18	4254.0
19	6075.0
20	6075.0
21	6075.0
22	10543.5
23	15012.0
24	15012.0
25	15012.0
26	23259.5
27	31507.0
28	31507.0
29	31507.0
30	39411.5
31	47316.0
32	47316.0
33	47316.0
34	61762.0
35	76208.0
36	76208.0
37	76208.0
38	89724.5
39	103241.0
40	103241.0
41	103241.0
42	121590.5
43	139940.0
44	139940.0
45	139940.0
46	159304.0
47	178668.0
48	178668.0
49	178668.0
50	188057.5
51	197447.0
52	197447.0
53	197447.0
54	185856.0
55	174265.0
56	174265.0
57	174265.0
58	163548.0
59	152831.0
60	152831.0
61	152831.0
62	136241.0
63	119651.0
64	119651.0
65	119651.0
66	99858.0
67	80065.0
68	80065.0
69	80065.0
70	60969.5
71	41874.0
72	41874.0
73	41874.0
74	32708.0
75	23542.0
76	23542.0
77	23542.0
78	19292.0
79	15042.0
80	15042.0
81	15042.0
82	10429.0
83	5816.0
84	5816.0
85	5816.0
86	4541.0
87	3266.0
88	3266.0
89	3266.0
90	2343.0
91	1420.0
92	1420.0
93	1420.0
94	983.0
95	546.0
96	546.0
97	546.0
98	688.0
99	830.0
100	830.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038272206850021494
2	0.005698619034653935
3	0.0014774197497250945
4	0.003165899463696631
5	0.009427345069674412
6	0.013015364461863927
7	0.021950236281629974
8	0.037990793564359575
9	0.0495287382764984
10	0.057337956953616764
11	0.05600124384672262
12	0.06359940255959454
13	0.06359940255959454
14	0.06732812859461502
15	0.06022244313165147
16	0.06697636198753762
17	0.06254410273836233
18	0.07548911387881078
19	0.07351922087917731
20	0.07759971352127519
21	0.07253427437936058
22	0.0764740603786275
23	0.08104702627063375
24	0.07598158712871915
25	0.0768961803071204
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1421397.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.789095249439676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.4598159098608	39.34113968249982
2	13.364765337445142	13.575686794198221
3	4.083447679827115	6.2218383947052835
4	1.6724446717722325	3.3976780693623114
5	0.9217014702735014	2.3406191882634726
6	0.5607326160716988	1.7087461348279795
7	0.3618018379853743	1.286291160760306
8	0.2599607822397115	1.056253834423324
9	0.19649547935781425	0.89818448554695
>10	0.9323936642210954	8.326907392732387
>50	0.07491753306308055	2.6656799528857777
>100	0.09219424962134906	10.300982645591914
>500	0.01362747690353459	4.7693921898121925
>1k	0.005701291357601205	4.110600074390118
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3044	0.2141555103887232	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2102	0.1478826816153404	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1899	0.13360095736799782	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1879	0.13219389093968822	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1809	0.12726915844060455	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1791	0.1260027986551259	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1771	0.12459573222681629	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1748	0.12297760583426023	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1721	0.12107806615604226	No Hit
GTACATGGGGAATAATTGCAATCCC	1643	0.11559050708563477	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1627	0.11446485394298708	No Hit
TCGTAGTTCCGACCATAAACGATGC	1614	0.11355026076458584	No Hit
GAACTACGACGGTATCTGATCGTCT	1593	0.11207284101486072	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1566	0.11017330133664274	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1509	0.10616316201596035	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1475	0.10377114908783401	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4070664283096136E-4
2	0.0	0.0	0.0	0.0	1.4070664283096136E-4
3	0.0	0.0	0.0	0.0	1.4070664283096136E-4
4	0.0	0.0	0.0	0.0	1.4070664283096136E-4
5	0.0	0.0	0.0	0.0	1.4070664283096136E-4
6	0.0	0.0	0.0	0.0	2.1105996424644206E-4
7	0.0	0.0	0.0	0.0	2.1105996424644206E-4
8	0.0	0.0	0.0	0.0	2.1105996424644206E-4
9	0.0	0.0	0.0	0.0	2.1105996424644206E-4
10	0.0	0.0	0.0	7.035332141548068E-5	2.814132856619227E-4
11	0.0	0.0	0.0	7.035332141548068E-5	2.814132856619227E-4
12	0.0	0.0	0.0	1.4070664283096136E-4	3.517666070774034E-4
13	0.0	0.0	0.0	1.4070664283096136E-4	4.2211992849288413E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGACAA	25	0.0060285367	19.001797	13
CGTTATT	205	0.0	15.287132	2
TACTAGA	50	8.740128E-5	15.194481	2
CGAACGA	135	0.0	14.780216	16
GCGTTAT	200	0.0	14.722764	1
TCGGCAA	55	1.9583944E-4	13.819488	13
CGACCAT	345	0.0	13.494028	10
ACGCGCG	85	2.6953603E-7	13.413033	13
GTTATTC	235	0.0	12.931474	3
TAACACA	105	2.0019797E-8	12.662514	4
CTAGGAC	190	0.0	12.495462	3
CCGACCA	365	0.0	12.494331	9
GTCTTAG	130	1.9826984E-10	12.421189	1
TCGAACG	140	5.2750693E-11	12.209851	3
ACGAACG	165	0.0	12.092903	15
TTATGTT	55	0.003075767	12.086945	4
GTCCTAC	215	0.0	11.928419	1
TAACGAA	185	0.0	11.811928	13
CGAGATT	130	2.6266207E-9	11.688474	4
AAGACGG	375	0.0	11.650743	5
>>END_MODULE