FastQCFastQC Report
Thu 2 Feb 2017
SRR4063976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421397
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19190.13500802379630744No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG18640.131138591118456No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18200.12804304497617483No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA18120.127480218404851No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT17520.12325901911992215No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17440.12269619254859832No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC16850.11854534658508496No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15890.1117914277291988No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC15750.11080648122938207No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA15510.10911800151541054No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA15020.10567068876605198No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14720.10356008912358756No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCG450.001399103124.44213916
GTATCAA24450.020.7869721
TTCGCGC654.9358886E-420.30577717
TCGCGCA654.9358886E-420.30577718
CGTTATA851.4317052E-418.11657542
GTCCTAC2200.018.0014691
GGTATCA13500.017.438461
CGAGCCG7000.017.285315
ATTTCGT1206.411732E-616.49902342
TAATACC953.3483398E-416.2095684
GTAATAC953.3483398E-416.2095683
AGGCCCG7250.016.08180610
TCCTATA1105.6285906E-515.9979292
TATACTG1958.931238E-1015.7939385
AACGCAG31700.015.7530116
GCTATAT850.002978665315.5306791
TCCTACA3750.015.2513582
TTGATTA6400.015.12623436
GTATAAA1603.821242E-715.1262341
CGACGGT6050.014.908217
Produced by FastQC (version 0.11.5)