FastQCFastQC Report
Thu 2 Feb 2017
SRR4063975_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063975_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2442538
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35520.14542250724451372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7800.014.2492471
TAGGCCG551.9624337E-413.8168865
GTATCAA22650.013.2951041
TCTTATA1800.012.6641822
CTAATCA951.3626905E-510.9987434
ACGGTAT2700.010.9077259
CGACCAT3500.010.58558810
TCGCGTA1803.45608E-1110.5558629
GCGTTAT2350.010.5100831
CAAGACG5450.010.45752054
TCGGCGT2550.010.43167713
CGGTCCA3750.010.38654310
GTGTTAA1856.002665E-1110.2697281
CGGACAT2500.010.2590375
CCGGTCC4100.010.1954189
TCGCACG1409.676296E-810.17991118
GTCGGCG2800.010.17886812
CGAACGA2350.010.10729816
CTAATAG856.608967E-410.0566443
GTAGAAC2650.010.0355623
Produced by FastQC (version 0.11.5)