##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063975_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2442538
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244317181554596	32.0	32.0	32.0	32.0	32.0
2	30.784391071909628	32.0	32.0	32.0	32.0	32.0
3	30.798484609041907	32.0	32.0	32.0	32.0	32.0
4	30.837334362863547	32.0	32.0	32.0	32.0	32.0
5	30.71322206655536	32.0	32.0	32.0	32.0	32.0
6	34.37313646706827	36.0	36.0	36.0	32.0	36.0
7	34.32763830081661	36.0	36.0	36.0	32.0	36.0
8	34.278030474858525	36.0	36.0	36.0	32.0	36.0
9	34.45613824636505	36.0	36.0	36.0	32.0	36.0
10	34.09692295472988	36.0	36.0	36.0	32.0	36.0
11	34.416250637656404	36.0	36.0	36.0	32.0	36.0
12	34.21280242108823	36.0	36.0	36.0	32.0	36.0
13	34.30693360758359	36.0	36.0	36.0	32.0	36.0
14	34.19650011586309	36.0	36.0	36.0	32.0	36.0
15	34.114312653477654	36.0	36.0	36.0	32.0	36.0
16	34.120198334683025	36.0	36.0	36.0	32.0	36.0
17	34.04408406337998	36.0	36.0	36.0	32.0	36.0
18	34.0341272070281	36.0	36.0	36.0	32.0	36.0
19	34.058952614043264	36.0	36.0	36.0	32.0	36.0
20	34.04189249051601	36.0	36.0	36.0	32.0	36.0
21	34.032144433372174	36.0	36.0	36.0	32.0	36.0
22	34.00879822545238	36.0	36.0	36.0	32.0	36.0
23	33.93486938586012	36.0	36.0	36.0	32.0	36.0
24	33.92651741753864	36.0	36.0	36.0	32.0	36.0
25	33.502467515346744	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	26.0
5	39.0
6	100.0
7	36.0
8	138.0
9	162.0
10	226.0
11	74.0
12	165.0
13	118.0
14	200.0
15	387.0
16	635.0
17	837.0
18	1102.0
19	1644.0
20	2463.0
21	3799.0
22	5841.0
23	9244.0
24	14013.0
25	20296.0
26	29174.0
27	38120.0
28	51535.0
29	70140.0
30	91170.0
31	123245.0
32	174003.0
33	244265.0
34	516048.0
35	1043289.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.70465356502449	16.71352260840147	11.26909973516563	26.312724091408406
2	17.257201674733807	19.030649135405977	36.68340144889204	27.02874774096817
3	19.426798531991096	22.41571313232551	27.891514076607006	30.265974259076383
4	13.207398848853233	15.157502720646699	34.552008365425635	37.08309006507443
5	15.31219649610123	35.66134983794018	33.30312966259783	15.723324003360755
6	35.58468678919255	34.25015508526106	16.343222620541685	13.821935505004697
7	31.289578862880635	29.70924994676408	19.97796851709283	19.023202673262464
8	28.644184626373526	32.66478895390484	18.722464524621532	19.968561895100105
9	27.330966859813067	14.292028184683241	17.971749778603925	40.40525517689977
10	16.349939945500335	26.32799916103946	31.030684326349096	26.291376567111108
11	37.51071720147191	20.850297541874404	21.855676522190805	19.783308734462874
12	24.97641280439568	23.12643105553878	28.224133050218708	23.673023089846833
13	29.889514916858634	19.225895681335604	24.616882763073427	26.267706638732335
14	23.981455913959113	19.021494653990395	24.578083531963394	32.4189659000871
15	25.515186869944813	26.74272937613885	21.67336595309571	26.06871780082063
16	26.34452345895083	25.399381048481256	22.979169175984943	25.27692631658297
17	24.70958433156465	25.505288031384065	24.46476502918495	25.320362607866336
18	25.41217385318789	24.79045601245894	25.12958600901315	24.66778412534002
19	26.027061631277594	24.579827715125628	24.601747431603314	24.791363221993464
20	26.22201207405046	24.087910953263247	24.27356156626674	25.416515406419553
21	26.978950197649187	23.92985092727952	23.948698072019038	25.142500803052254
22	26.5599628951456	23.721662785871477	24.31912633145829	25.399247987524642
23	25.208897841344584	23.888538102318865	24.75699095325761	26.145573103078938
24	25.284183096514084	24.4653948899487	24.528574003966106	25.7218480095711
25	25.383511966196124	24.11029444000349	24.717997170445617	25.788196423354766
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	71.0
1	71.0
2	225.0
3	379.0
4	379.0
5	379.0
6	1019.5
7	1660.0
8	1660.0
9	1660.0
10	2103.0
11	2546.0
12	2546.0
13	2546.0
14	3726.0
15	4906.0
16	4906.0
17	4906.0
18	8579.0
19	12252.0
20	12252.0
21	12252.0
22	19475.5
23	26699.0
24	26699.0
25	26699.0
26	40321.5
27	53944.0
28	53944.0
29	53944.0
30	69774.0
31	85604.0
32	85604.0
33	85604.0
34	109583.0
35	133562.0
36	133562.0
37	133562.0
38	159534.0
39	185506.0
40	185506.0
41	185506.0
42	217740.5
43	249975.0
44	249975.0
45	249975.0
46	281482.0
47	312989.0
48	312989.0
49	312989.0
50	326966.0
51	340943.0
52	340943.0
53	340943.0
54	325296.5
55	309650.0
56	309650.0
57	309650.0
58	287368.0
59	265086.0
60	265086.0
61	265086.0
62	231680.5
63	198275.0
64	198275.0
65	198275.0
66	162787.0
67	127299.0
68	127299.0
69	127299.0
70	96647.0
71	65995.0
72	65995.0
73	65995.0
74	50000.0
75	34005.0
76	34005.0
77	34005.0
78	26051.0
79	18097.0
80	18097.0
81	18097.0
82	12403.0
83	6709.0
84	6709.0
85	6709.0
86	4978.5
87	3248.0
88	3248.0
89	3248.0
90	2315.5
91	1383.0
92	1383.0
93	1383.0
94	960.5
95	538.0
96	538.0
97	538.0
98	877.5
99	1217.0
100	1217.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04143231343790762
2	0.004912922542044382
3	0.0018832869744503464
4	0.004257866203105131
5	0.009416434872251732
6	0.01363336005417316
7	0.02366391024418044
8	0.03746103438308841
9	0.04962051767464826
10	0.05821813212322592
11	0.05604825800048965
12	0.06530092878800658
13	0.0644821083643325
14	0.06763456699547765
15	0.06067459339424812
16	0.0669795106565384
17	0.06362234691947474
18	0.07701006084654569
19	0.07422607140605386
20	0.07909805292691455
21	0.07574088918985088
22	0.0772557069736479
23	0.08151357317675303
24	0.07606841735932052
25	0.07660065063470865
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2442538.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.47971857098229
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.87383020755775	37.291301586380456
2	14.357357392535727	14.495107211964312
3	4.911099261925451	7.437327258484667
4	2.228762975669576	4.500293110929009
5	1.1844625857858384	2.9895668994163547
6	0.7548305499468387	2.2862180238057728
7	0.5123025622091391	1.8102622414457343
8	0.36212553068733844	1.4623995901171563
9	0.2641799768604265	1.2002157795602648
>10	1.3942402678050292	12.465348320929559
>50	0.08675427910780831	3.0200253701864845
>100	0.05996110748493373	6.301752055559881
>500	0.007000193616822663	2.4510166115901884
>1k	0.0030931088074321557	2.289165939630112
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3552	0.14542250724451372	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.188204236740636E-5
2	0.0	0.0	0.0	0.0	8.188204236740636E-5
3	0.0	0.0	0.0	0.0	8.188204236740636E-5
4	0.0	0.0	0.0	0.0	8.188204236740636E-5
5	0.0	0.0	0.0	4.094102118370318E-5	8.188204236740636E-5
6	0.0	0.0	0.0	4.094102118370318E-5	1.2282306355110954E-4
7	0.0	0.0	0.0	4.094102118370318E-5	1.2282306355110954E-4
8	0.0	0.0	0.0	4.094102118370318E-5	1.2282306355110954E-4
9	0.0	0.0	0.0	8.188204236740636E-5	1.2282306355110954E-4
10	0.0	0.0	0.0	8.188204236740636E-5	1.2282306355110954E-4
11	0.0	0.0	0.0	8.188204236740636E-5	1.2282306355110954E-4
12	0.0	0.0	0.0	8.188204236740636E-5	2.0470510591851591E-4
13	0.0	0.0	0.0	8.188204236740636E-5	2.456461271022191E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	780	0.0	14.249247	1
TAGGCCG	55	1.9624337E-4	13.816886	5
GTATCAA	2265	0.0	13.295104	1
TCTTATA	180	0.0	12.664182	2
CTAATCA	95	1.3626905E-5	10.998743	4
ACGGTAT	270	0.0	10.907725	9
CGACCAT	350	0.0	10.585588	10
TCGCGTA	180	3.45608E-11	10.555862	9
GCGTTAT	235	0.0	10.510083	1
CAAGACG	545	0.0	10.4575205	4
TCGGCGT	255	0.0	10.431677	13
CGGTCCA	375	0.0	10.386543	10
GTGTTAA	185	6.002665E-11	10.269728	1
CGGACAT	250	0.0	10.259037	5
CCGGTCC	410	0.0	10.195418	9
TCGCACG	140	9.676296E-8	10.179911	18
GTCGGCG	280	0.0	10.178868	12
CGAACGA	235	0.0	10.107298	16
CTAATAG	85	6.608967E-4	10.056644	3
GTAGAAC	265	0.0	10.035562	3
>>END_MODULE