FastQCFastQC Report
Thu 2 Feb 2017
SRR4063975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2442538
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25550.029.1030541
GGTATCA10900.024.2196311
TCAACGC37550.019.7434864
ATCAACG38550.019.28843
TATCAAC38700.019.2132432
CAACGCA38750.019.1888475
AACGCAG40100.018.652566
TAGGACG2251.8189894E-1216.6215044
CGCAATA5600.016.10842336
TTAACGG1105.621186E-516.00127435
ACCGTCG6800.015.8522568
TATACCG1259.45196E-615.83931355
TCTAGCG5700.015.82549728
CTAGCGG5700.015.82517229
ATACCGT7150.015.6916296
TACCGTC7350.015.5639527
TACTACG850.002982371715.5284212
AGAGTAC48600.015.29974911
ACGCAGA49100.015.1887517
CAGAGTA49900.015.07781310
Produced by FastQC (version 0.11.5)