##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063975_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2442538
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.356324446129396	32.0	32.0	32.0	32.0	32.0
2	31.408034593525258	32.0	32.0	32.0	32.0	32.0
3	31.48004002394231	32.0	32.0	32.0	32.0	32.0
4	31.56085104919555	32.0	32.0	32.0	32.0	32.0
5	31.512777692711435	32.0	32.0	32.0	32.0	32.0
6	35.10622925825514	36.0	36.0	36.0	36.0	36.0
7	35.12129227876905	36.0	36.0	36.0	36.0	36.0
8	35.063867583636366	36.0	36.0	36.0	36.0	36.0
9	35.17134963713973	36.0	36.0	36.0	36.0	36.0
10	35.02830252794429	36.0	36.0	36.0	36.0	36.0
11	35.16341240136285	36.0	36.0	36.0	36.0	36.0
12	35.08285070692861	36.0	36.0	36.0	36.0	36.0
13	35.11859508429347	36.0	36.0	36.0	36.0	36.0
14	35.05575716733988	36.0	36.0	36.0	36.0	36.0
15	35.036463711107054	36.0	36.0	36.0	36.0	36.0
16	35.04241407912589	36.0	36.0	36.0	36.0	36.0
17	35.015349607662195	36.0	36.0	36.0	36.0	36.0
18	35.00692681137407	36.0	36.0	36.0	36.0	36.0
19	35.01115847532362	36.0	36.0	36.0	36.0	36.0
20	35.001999968884824	36.0	36.0	36.0	36.0	36.0
21	34.99928680741098	36.0	36.0	36.0	36.0	36.0
22	34.98680634651334	36.0	36.0	36.0	36.0	36.0
23	34.92631353125315	36.0	36.0	36.0	32.0	36.0
24	34.90000728750177	36.0	36.0	36.0	32.0	36.0
25	34.87854191009516	36.0	36.0	36.0	32.0	36.0
26	34.815411674250306	36.0	36.0	36.0	32.0	36.0
27	34.80957553168057	36.0	36.0	36.0	32.0	36.0
28	34.79028166603754	36.0	36.0	36.0	32.0	36.0
29	34.765604874929274	36.0	36.0	36.0	32.0	36.0
30	34.749248527556176	36.0	36.0	36.0	32.0	36.0
31	34.74015225146958	36.0	36.0	36.0	32.0	36.0
32	34.711996701791335	36.0	36.0	36.0	32.0	36.0
33	34.68854118134498	36.0	36.0	36.0	32.0	36.0
34	34.69050880682307	36.0	36.0	36.0	32.0	36.0
35	34.65154032403999	36.0	36.0	36.0	32.0	36.0
36	34.627523092783	36.0	36.0	36.0	32.0	36.0
37	34.61984501367021	36.0	36.0	36.0	32.0	36.0
38	34.5928112479724	36.0	36.0	36.0	32.0	36.0
39	34.589860628575686	36.0	36.0	36.0	32.0	36.0
40	34.57378800247939	36.0	36.0	36.0	32.0	36.0
41	34.55693585933975	36.0	36.0	36.0	32.0	36.0
42	34.511378738017584	36.0	36.0	36.0	32.0	36.0
43	34.49584694281113	36.0	36.0	36.0	32.0	36.0
44	34.44411837195573	36.0	36.0	36.0	32.0	36.0
45	34.429785739259735	36.0	36.0	36.0	32.0	36.0
46	34.41144416176944	36.0	36.0	36.0	32.0	36.0
47	34.395317084114964	36.0	36.0	36.0	32.0	36.0
48	34.35395764569476	36.0	36.0	36.0	32.0	36.0
49	34.34450436390345	36.0	36.0	36.0	32.0	36.0
50	33.737877568332614	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	14.0
20	18.0
21	95.0
22	255.0
23	784.0
24	1929.0
25	4289.0
26	9110.0
27	16032.0
28	27029.0
29	41197.0
30	59034.0
31	85193.0
32	122333.0
33	197903.0
34	417421.0
35	1459898.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.71741564867759	17.574063951619674	11.822266988492474	26.886253411210266
2	16.505556331163316	19.709939984335907	36.23559843097052	27.548905253530254
3	18.552998595307237	22.997371165190806	28.300076109701138	30.149554129800816
4	12.805286959711578	15.736418430337624	34.65571467055988	36.80257993939091
5	14.932705243480346	36.039971537802074	33.11105088231995	15.916272336397633
6	35.6599100113407	34.580537393604175	16.106291427331495	13.653261167723633
7	31.252530669980167	29.83858982573433	19.945343669589178	18.96353583469633
8	28.58710898254193	32.81885481413186	18.56892298093213	20.025113222394083
9	27.307777025361702	14.109302674061269	17.92379289761073	40.659127402966305
10	16.193834554205583	26.661974783379105	31.053675799871932	26.09051486254338
11	37.79462182369323	20.767087349306337	21.754584780257257	19.683706046743183
12	24.897319834794658	23.167028039709546	28.21980924743791	23.715842878057888
13	29.959480522032695	19.17475698343807	24.672665094252718	26.193097400276518
14	23.94177522196211	18.969889467249995	24.527396548011204	32.56093876277669
15	25.58574861920301	26.848384600582207	21.54612414685375	26.019742633361037
16	26.41893587639308	25.418738129560616	22.89662671743943	25.265699276606878
17	24.84457758469984	25.554699887862498	24.360654516185427	25.240068011252237
18	25.457968137929228	24.73576556747763	25.10411344312122	24.70215285147192
19	26.00566542083088	24.6374978147721	24.582841529115015	24.773995235282005
20	26.295219691138662	23.97630316230778	24.275502350031935	25.452974796521627
21	27.03978402792505	23.983946206773446	23.99770238989117	24.978567375410332
22	26.555391609376482	23.817745163223798	24.293234572591764	25.333628654807956
23	25.288032088131825	23.84550728897947	24.786501498655284	26.079959124233426
24	25.449258083943395	24.390272860852715	24.471008918222807	25.689460136981086
25	25.467555267325338	24.029311047896982	24.732527271327807	25.77060641344987
26	25.321176567887832	24.731475999682285	24.828305954817054	25.11904147761283
27	25.880595254960113	24.338043762196087	24.245961524446216	25.535399458397578
28	25.412897036852943	24.178719860532738	24.933171254775456	25.475211847838857
29	25.351928724581562	24.466308477200734	24.68489876432413	25.496864033893573
30	25.256534710744138	24.548108728219166	24.889772880341393	25.3055836806953
31	25.693858148559713	24.281452596220536	24.29570112162099	25.72898813359876
32	25.729573617353697	24.291184194257088	24.01949023400236	25.959751954386856
33	25.243508686883242	24.162244136753007	24.494496846977054	26.0997503293867
34	25.831393354093773	24.152802945875194	24.702493299804377	25.31331040022666
35	25.921896904109946	24.070774431066333	24.823456968045384	25.183871696778336
36	25.17397107715488	24.474893136147006	24.5882261419283	25.76290964476981
37	26.02564233790321	24.195222410330633	24.324150594998045	25.454984656768115
38	25.28299646446346	24.185403782020966	24.646436109558643	25.885163643956933
39	25.91114102664885	24.213120365275266	24.115021421271912	25.760717186803973
40	25.985737482220983	24.256310988067074	24.560263600282664	25.19768792942928
41	25.181936504518166	24.597692584523966	24.908443204899296	25.311927706058572
42	26.114569101789936	24.413355054327734	24.513048359236535	24.959027484645798
43	25.684102440648072	23.743063991218857	24.61626517344679	25.95656839468628
44	25.24306982822574	24.270954281418934	24.59160829770056	25.894367592654767
45	25.428688287330775	24.304516380492355	24.547831758245312	25.718963573931557
46	25.293007172519168	24.2231272085312	24.730104232641985	25.75376138630765
47	25.38145530295338	24.141039792211878	24.964953768452496	25.512551136382243
48	25.769165419644334	24.713846758526664	24.216689150598672	25.300298671230326
49	25.289966207115093	24.615336745647742	24.417754406708465	25.6769426405287
50	25.23376538835208	24.782508834139268	24.48265372589624	25.501072051612418
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	18.0
2	31.0
3	94.0
4	157.0
5	189.0
6	221.0
7	224.5
8	228.0
9	305.0
10	382.0
11	571.0
12	760.0
13	1341.0
14	1922.0
15	2621.0
16	3320.0
17	3384.0
18	3448.0
19	3370.0
20	3292.0
21	3973.0
22	4654.0
23	5715.0
24	6776.0
25	8711.5
26	10647.0
27	13380.5
28	16114.0
29	19234.0
30	22354.0
31	26106.5
32	29859.0
33	35249.0
34	40639.0
35	48299.5
36	55960.0
37	66690.0
38	77420.0
39	86283.5
40	95147.0
41	105718.0
42	116289.0
43	123042.0
44	129795.0
45	142662.0
46	155529.0
47	166546.0
48	177563.0
49	186867.0
50	196171.0
51	194698.0
52	193225.0
53	194099.0
54	194973.0
55	193000.0
56	191027.0
57	185624.0
58	180221.0
59	166702.5
60	153184.0
61	134245.0
62	115306.0
63	100538.0
64	85770.0
65	72764.5
66	59759.0
67	51238.5
68	42718.0
69	36744.5
70	30771.0
71	23607.5
72	16444.0
73	13911.5
74	11379.0
75	9034.5
76	6690.0
77	5765.5
78	4841.0
79	4056.0
80	3271.0
81	2618.5
82	1966.0
83	1576.5
84	1187.0
85	872.5
86	558.0
87	404.0
88	250.0
89	184.0
90	118.0
91	93.5
92	69.0
93	62.0
94	55.0
95	43.0
96	31.0
97	27.0
98	23.0
99	21.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00912984772396581
2	3.6846919065332864E-4
3	4.50351233020735E-4
4	0.0
5	0.0
6	3.2752816946962545E-4
7	1.2282306355110954E-4
8	0.0
9	0.0014738767626133146
10	9.0070246604147E-4
11	0.0
12	8.188204236740636E-5
13	3.6846919065332864E-4
14	1.6376408473481273E-4
15	0.004421630287839944
16	8.597614448577668E-4
17	4.094102118370318E-5
18	4.0941021183703183E-4
19	4.094102118370318E-5
20	7.369383813066573E-4
21	0.0
22	8.188204236740636E-5
23	6.141153177555477E-4
24	4.50351233020735E-4
25	0.0018832869744503464
26	0.004585394372574756
27	0.006386799304657697
28	0.004135043139554021
29	0.0028249304616755192
30	0.0033162227158799575
31	0.007369383813066573
32	0.0034390457794310674
33	0.004912922542044382
34	0.002947753525226629
35	0.0030296355675940353
36	0.007287501770699166
37	0.0038075149700843957
38	0.007901617088454714
39	0.004339748245472537
40	0.0021289331015525654
41	0.0026611663769407065
42	0.0019242279956340495
43	0.0012691716566947985
44	0.0012282306355110954
45	0.0011872896143273921
46	8.597614448577668E-4
47	0.002047051059185159
48	0.0021698741227362687
49	4.912922542044382E-4
50	0.0010235255295925796
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2442538.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.17537554843001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.80801349055577	39.77944211392322
2	14.247236211442104	15.152042721412503
3	4.6547165125538745	7.425488958795921
4	2.033135980796484	4.324510772795145
5	1.119282012810992	2.975912068791357
6	0.697656675244526	2.225889342599805
7	0.4685865074881953	1.74420844588284
8	0.34546083322910026	1.4696007635384767
9	0.23571470439594316	1.12807961356873
>10	1.2586806682401377	11.736419202122537
>50	0.07338260658106513	2.6972613470020743
>100	0.049770041153901406	5.429275544213433
>500	0.005575837005365889	1.9269437127283744
>1k	0.0027879185026793716	1.9849253926255237
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2282306355110954E-4
2	0.0	0.0	0.0	0.0	1.6376408473481273E-4
3	0.0	0.0	0.0	0.0	1.6376408473481273E-4
4	0.0	0.0	0.0	0.0	1.6376408473481273E-4
5	0.0	0.0	0.0	4.094102118370318E-5	1.6376408473481273E-4
6	0.0	0.0	0.0	4.094102118370318E-5	2.456461271022191E-4
7	0.0	0.0	0.0	4.094102118370318E-5	2.456461271022191E-4
8	0.0	0.0	0.0	4.094102118370318E-5	2.456461271022191E-4
9	0.0	0.0	0.0	4.094102118370318E-5	2.456461271022191E-4
10	0.0	0.0	0.0	4.094102118370318E-5	2.8658714828592226E-4
11	0.0	0.0	0.0	4.094102118370318E-5	2.8658714828592226E-4
12	0.0	0.0	0.0	4.094102118370318E-5	4.912922542044382E-4
13	0.0	0.0	0.0	4.094102118370318E-5	6.550563389392509E-4
14	0.0	0.0	0.0	4.094102118370318E-5	6.550563389392509E-4
15	0.0	0.0	0.0	4.094102118370318E-5	6.959973601229541E-4
16	0.0	0.0	0.0	1.2282306355110954E-4	6.959973601229541E-4
17	0.0	0.0	0.0	1.6376408473481273E-4	6.959973601229541E-4
18	0.0	0.0	0.0	2.0470510591851591E-4	7.369383813066573E-4
19	0.0	0.0	0.0	2.456461271022191E-4	7.778794024903605E-4
20	0.0	0.0	0.0	2.8658714828592226E-4	8.188204236740637E-4
21	0.0	0.0	0.0	2.8658714828592226E-4	8.188204236740637E-4
22	0.0	0.0	0.0	3.6846919065332864E-4	8.188204236740637E-4
23	0.0	0.0	0.0	4.50351233020735E-4	8.597614448577668E-4
24	0.0	0.0	0.0	5.731742965718445E-4	8.597614448577668E-4
25	0.0	0.0	0.0	6.959973601229541E-4	8.597614448577668E-4
26	0.0	0.0	0.0	8.188204236740637E-4	9.0070246604147E-4
27	0.0	0.0	0.0	0.0011872896143273924	0.0010235255295925796
28	0.0	0.0	0.0	0.0027021073981244098	0.0010235255295925796
29	0.0	0.0	0.0	0.006018330114004367	0.0010235255295925796
30	0.0	0.0	0.0	0.013674301075356863	0.0010235255295925796
31	0.0	0.0	0.0	0.03275281694696255	0.0010235255295925796
32	0.0	0.0	0.0	0.054246853068406715	0.0010235255295925796
33	0.0	0.0	0.0	0.07569994816866718	0.0010235255295925796
34	0.0	0.0	0.0	0.10153373253558388	0.0010644665507762827
35	0.0	4.094102118370318E-5	0.0	0.13211667535981017	0.0010644665507762827
36	0.0	4.094102118370318E-5	0.0	0.17412216309428963	0.0010644665507762827
37	0.0	4.094102118370318E-5	0.0	0.23897274064927546	0.0010644665507762827
38	0.0	4.094102118370318E-5	0.0	0.3299436897194639	0.001105407571959986
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2555	0.0	29.103054	1
GGTATCA	1090	0.0	24.219631	1
TCAACGC	3755	0.0	19.743486	4
ATCAACG	3855	0.0	19.2884	3
TATCAAC	3870	0.0	19.213243	2
CAACGCA	3875	0.0	19.188847	5
AACGCAG	4010	0.0	18.65256	6
TAGGACG	225	1.8189894E-12	16.621504	4
CGCAATA	560	0.0	16.108423	36
TTAACGG	110	5.621186E-5	16.001274	35
ACCGTCG	680	0.0	15.852256	8
TATACCG	125	9.45196E-6	15.8393135	5
TCTAGCG	570	0.0	15.825497	28
CTAGCGG	570	0.0	15.825172	29
ATACCGT	715	0.0	15.691629	6
TACCGTC	735	0.0	15.563952	7
TACTACG	85	0.0029823717	15.528421	2
AGAGTAC	4860	0.0	15.299749	11
ACGCAGA	4910	0.0	15.188751	7
CAGAGTA	4990	0.0	15.077813	10
>>END_MODULE