FastQCFastQC Report
Thu 2 Feb 2017
SRR4063974_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063974_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005288
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24760.2462975784053923No Hit
TATCAACGCAGAGTACTTTTTTTTT17860.17766053111148247No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13490.13419040115867292No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTT250.006028252719.0012957
GCGTTAT508.718788E-515.19800951
GGTATAC602.5696487E-514.2481351
GTATTAA551.9612446E-413.8163711
AACCGCG707.251325E-613.5723537
TTGCGCG852.6919224E-713.41334618
TTAGGGT500.001502073313.2962724
AGAACCG1301.4551915E-1113.150165
GTATTAG1457.2759576E-1212.4466461
CGTCGTA1301.9645086E-1012.42454210
GTCCTAG853.9451643E-612.2925081
GCACCGT701.0909869E-412.2132946
TTAACCT701.0930166E-412.2108624
AAGACCG550.003073593612.0875215
CCTAACC550.003075885612.0863183
CGCGGTC802.8605431E-511.876410
GTAATAA802.8678396E-511.8734451
CGAACGA907.443323E-611.61248116
GAACCGC907.459843E-611.6101686
TAAATCC1406.82121E-1011.5324814
Produced by FastQC (version 0.11.5)