##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063974_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1005288
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.286146855428495	32.0	32.0	32.0	32.0	32.0
2	30.91493382990745	32.0	32.0	32.0	32.0	32.0
3	30.93900852293074	32.0	32.0	32.0	32.0	32.0
4	30.96463202584732	32.0	32.0	32.0	32.0	32.0
5	30.892218946212427	32.0	32.0	32.0	32.0	32.0
6	34.552009971271914	36.0	36.0	36.0	32.0	36.0
7	34.49739975012136	36.0	36.0	36.0	32.0	36.0
8	34.458412912518604	36.0	36.0	36.0	32.0	36.0
9	34.57876648283875	36.0	36.0	36.0	32.0	36.0
10	34.30711099704761	36.0	36.0	36.0	32.0	36.0
11	34.547520710482964	36.0	36.0	36.0	32.0	36.0
12	34.38147774568084	36.0	36.0	36.0	32.0	36.0
13	34.46212030781229	36.0	36.0	36.0	32.0	36.0
14	34.35831522906869	36.0	36.0	36.0	32.0	36.0
15	34.30695780711597	36.0	36.0	36.0	32.0	36.0
16	34.304234209500166	36.0	36.0	36.0	32.0	36.0
17	34.23213447290727	36.0	36.0	36.0	32.0	36.0
18	34.22853948321277	36.0	36.0	36.0	32.0	36.0
19	34.238880798338386	36.0	36.0	36.0	32.0	36.0
20	34.220750670454635	36.0	36.0	36.0	32.0	36.0
21	34.20571617287782	36.0	36.0	36.0	32.0	36.0
22	34.18048559218851	36.0	36.0	36.0	32.0	36.0
23	34.13564471076945	36.0	36.0	36.0	32.0	36.0
24	34.11889130279084	36.0	36.0	36.0	32.0	36.0
25	33.73265173761151	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	5.0
5	12.0
6	39.0
7	16.0
8	55.0
9	69.0
10	95.0
11	22.0
12	63.0
13	56.0
14	102.0
15	179.0
16	280.0
17	379.0
18	437.0
19	606.0
20	930.0
21	1351.0
22	2063.0
23	3262.0
24	4880.0
25	7096.0
26	10186.0
27	13760.0
28	18942.0
29	25574.0
30	33992.0
31	46258.0
32	65505.0
33	93842.0
34	205345.0
35	469882.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.733073802955936	16.96540523757777	11.07074193047611	26.23077902899018
2	16.996754005763805	19.576240078230153	37.25283241099878	26.174173505007264
3	19.344114548672145	23.429496840142967	28.117206672456174	29.109181938728717
4	12.836299282859965	15.182597640570489	35.37791359900044	36.6031894775691
5	14.88520115042584	36.15355459100889	33.4950601526274	15.466184105937863
6	34.47623709283805	34.942531480094196	16.846588450185493	13.734642976882263
7	30.60003323181379	30.085537096753328	20.64760248382934	18.666827187603538
8	28.190017872140555	32.70362896003248	19.467796313323703	19.63855685450326
9	27.60849639183004	14.137099511048435	18.3829068981681	39.87149719895343
10	16.122052494754904	26.417370325693607	31.534175733616788	25.926401445934705
11	37.39330615559784	20.837646206396855	22.470798531765304	19.298249106239997
12	25.09819183688908	23.350347279593418	28.693217447390474	22.858243436127033
13	29.79255813120168	19.227664489825983	25.072486704549867	25.907290674422473
14	23.64420175026477	19.46711684092339	25.114069803553086	31.774611605258755
15	25.342137165402296	27.079447484557797	22.106091882864177	25.472323467175734
16	25.73778764735099	25.457095392566035	23.8043953221939	25.00072163788907
17	24.172143573082984	25.64227365520921	25.200836491077194	24.984746280630613
18	24.976133615913977	24.706261802536655	26.065077830792067	24.2525267507573
19	25.700568497984722	24.58059461843144	25.327277052928743	24.39155983065509
20	25.94121599761081	24.126427913690552	24.892063413055922	25.040292675642718
21	26.77186517310271	23.915140402378317	24.45198327256149	24.86101115195749
22	26.06760945871419	24.01372178542488	24.918718530184144	24.99995022567678
23	24.6946715354627	24.030554748071662	25.285517455589492	25.989256260876143
24	24.93006763281177	24.633116385377615	25.258177358437973	25.178638623372642
25	24.920162144512492	24.316400805944237	25.293070631614995	25.470366417928275
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	279.0
1	279.0
2	384.0
3	489.0
4	489.0
5	489.0
6	1179.0
7	1869.0
8	1869.0
9	1869.0
10	1904.5
11	1940.0
12	1940.0
13	1940.0
14	2153.5
15	2367.0
16	2367.0
17	2367.0
18	4044.5
19	5722.0
20	5722.0
21	5722.0
22	9444.5
23	13167.0
24	13167.0
25	13167.0
26	19701.0
27	26235.0
28	26235.0
29	26235.0
30	33149.5
31	40064.0
32	40064.0
33	40064.0
34	49776.0
35	59488.0
36	59488.0
37	59488.0
38	69756.0
39	80024.0
40	80024.0
41	80024.0
42	92313.5
43	104603.0
44	104603.0
45	104603.0
46	117299.5
47	129996.0
48	129996.0
49	129996.0
50	134489.0
51	138982.0
52	138982.0
53	138982.0
54	129863.5
55	120745.0
56	120745.0
57	120745.0
58	110911.0
59	101077.0
60	101077.0
61	101077.0
62	88849.0
63	76621.0
64	76621.0
65	76621.0
66	62984.5
67	49348.0
68	49348.0
69	49348.0
70	37311.5
71	25275.0
72	25275.0
73	25275.0
74	19155.5
75	13036.0
76	13036.0
77	13036.0
78	10507.5
79	7979.0
80	7979.0
81	7979.0
82	5449.0
83	2919.0
84	2919.0
85	2919.0
86	2282.5
87	1646.0
88	1646.0
89	1646.0
90	1140.5
91	635.0
92	635.0
93	635.0
94	435.0
95	235.0
96	235.0
97	235.0
98	391.0
99	547.0
100	547.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03939169670780911
2	0.00487422509768345
3	0.002088953613292907
4	0.0044763291713419435
5	0.009450028250610771
6	0.012832143624513572
7	0.022580593819880472
8	0.03700432114976007
9	0.04824488106890762
10	0.05371595005610332
11	0.05411384598244483
12	0.0618728165461042
13	0.06177334256451882
14	0.06366334821464098
15	0.05709806543000613
16	0.06256913441720184
17	0.06117649867500657
18	0.0733123244284225
19	0.07072600090720271
20	0.0758986479496423
21	0.07072600090720271
22	0.07500338211537391
23	0.07918128934195971
24	0.07420759026269089
25	0.07480443415220314
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1005288.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.07480864279242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.7006217618719	47.67373787936952
2	11.907708920168664	14.068912516660726
3	3.279825925685234	5.812652668245742
4	1.332728757236652	3.149227852260071
5	0.7135391678671477	2.1076094900444544
6	0.42995749397052385	1.5239794008505965
7	0.31227162994114943	1.2913170748342404
8	0.20892399297090264	0.9873715924515348
9	0.16628362846989236	0.8840856179059273
>10	0.8042219408863112	8.501186761748002
>50	0.06951325258511482	2.91009071808487
>100	0.06814686836294014	8.115758581013273
>500	0.005749363228146653	2.4135597730093905
>1k	5.072967554247046E-4	0.560510073521613
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2476	0.2462975784053923	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1786	0.17766053111148247	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1349	0.13419040115867292	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	9.947398158537653E-5
7	0.0	0.0	0.0	0.0	9.947398158537653E-5
8	0.0	0.0	0.0	0.0	9.947398158537653E-5
9	0.0	0.0	0.0	0.0	9.947398158537653E-5
10	0.0	0.0	0.0	0.0	9.947398158537653E-5
11	0.0	0.0	0.0	9.947398158537653E-5	9.947398158537653E-5
12	0.0	0.0	0.0	9.947398158537653E-5	9.947398158537653E-5
13	0.0	0.0	0.0	9.947398158537653E-5	9.947398158537653E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTT	25	0.0060282527	19.001295	7
GCGTTAT	50	8.718788E-5	15.1980095	1
GGTATAC	60	2.5696487E-5	14.248135	1
GTATTAA	55	1.9612446E-4	13.816371	1
AACCGCG	70	7.251325E-6	13.572353	7
TTGCGCG	85	2.6919224E-7	13.413346	18
TTAGGGT	50	0.0015020733	13.296272	4
AGAACCG	130	1.4551915E-11	13.15016	5
GTATTAG	145	7.2759576E-12	12.446646	1
CGTCGTA	130	1.9645086E-10	12.424542	10
GTCCTAG	85	3.9451643E-6	12.292508	1
GCACCGT	70	1.0909869E-4	12.213294	6
TTAACCT	70	1.0930166E-4	12.210862	4
AAGACCG	55	0.0030735936	12.087521	5
CCTAACC	55	0.0030758856	12.086318	3
CGCGGTC	80	2.8605431E-5	11.8764	10
GTAATAA	80	2.8678396E-5	11.873445	1
CGAACGA	90	7.443323E-6	11.612481	16
GAACCGC	90	7.459843E-6	11.610168	6
TAAATCC	140	6.82121E-10	11.532481	4
>>END_MODULE