FastQCFastQC Report
Thu 2 Feb 2017
SRR4063974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005288
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG2650.022.4147158
CCTATAC803.615074E-621.9996263
GTATCAA17750.021.6908531
CGCAATA2350.020.5964236
GTACTAA755.470081E-520.5340061
GTATAGG1201.4302714E-820.1673281
ACGCAAC550.004482245520.00065640
CCGTCGT2750.020.0006569
GGTATAT550.004482245520.0006561
GTCGTAG2750.019.9996611
ATACCGT2750.019.999666
TACCGTC2850.019.2979187
GGCGTTA2400.019.24967442
CGGCGTT2450.018.85682341
CGTCGTA3050.018.7537810
CGAGCCG3350.018.38774715
CCTCGCG1801.4551915E-1118.33302313
TACACCG600.007411979618.3330235
TCCTATA1203.1628588E-718.3321112
TAGCGGC2800.018.0729230
Produced by FastQC (version 0.11.5)