##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4063974_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1005288 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.31661175702883 32.0 32.0 32.0 32.0 32.0 2 31.422888764214832 32.0 32.0 32.0 32.0 32.0 3 31.493326290575435 32.0 32.0 32.0 32.0 32.0 4 31.573198924089414 32.0 32.0 32.0 32.0 32.0 5 31.520436929516716 32.0 32.0 32.0 32.0 32.0 6 35.11358635535289 36.0 36.0 36.0 36.0 36.0 7 35.13216908985286 36.0 36.0 36.0 36.0 36.0 8 35.07453883862137 36.0 36.0 36.0 36.0 36.0 9 35.180236907234544 36.0 36.0 36.0 36.0 36.0 10 35.03818308783155 36.0 36.0 36.0 36.0 36.0 11 35.175691941015906 36.0 36.0 36.0 36.0 36.0 12 35.088521896212825 36.0 36.0 36.0 36.0 36.0 13 35.130760538273606 36.0 36.0 36.0 36.0 36.0 14 35.0780293806352 36.0 36.0 36.0 36.0 36.0 15 35.04172436157599 36.0 36.0 36.0 36.0 36.0 16 35.0534334439484 36.0 36.0 36.0 36.0 36.0 17 35.01944815813976 36.0 36.0 36.0 36.0 36.0 18 35.01800280118732 36.0 36.0 36.0 36.0 36.0 19 35.01276450131704 36.0 36.0 36.0 36.0 36.0 20 35.00312845672086 36.0 36.0 36.0 36.0 36.0 21 35.003002124764244 36.0 36.0 36.0 36.0 36.0 22 34.98893948798752 36.0 36.0 36.0 36.0 36.0 23 34.93003696453156 36.0 36.0 36.0 32.0 36.0 24 34.91079372279387 36.0 36.0 36.0 32.0 36.0 25 34.87919183358401 36.0 36.0 36.0 32.0 36.0 26 34.822262873922696 36.0 36.0 36.0 32.0 36.0 27 34.81485405177422 36.0 36.0 36.0 32.0 36.0 28 34.79015764636602 36.0 36.0 36.0 32.0 36.0 29 34.76107145415045 36.0 36.0 36.0 32.0 36.0 30 34.74275530992114 36.0 36.0 36.0 32.0 36.0 31 34.73144511821488 36.0 36.0 36.0 32.0 36.0 32 34.69353558383269 36.0 36.0 36.0 32.0 36.0 33 34.66819856598308 36.0 36.0 36.0 32.0 36.0 34 34.657805524387044 36.0 36.0 36.0 32.0 36.0 35 34.61200272956606 36.0 36.0 36.0 32.0 36.0 36 34.598001766657916 36.0 36.0 36.0 32.0 36.0 37 34.578217386460395 36.0 36.0 36.0 32.0 36.0 38 34.54131154455241 36.0 36.0 36.0 32.0 36.0 39 34.529662146568946 36.0 36.0 36.0 32.0 36.0 40 34.508604499407134 36.0 36.0 36.0 32.0 36.0 41 34.49729430770088 36.0 36.0 36.0 32.0 36.0 42 34.43086657753798 36.0 36.0 36.0 32.0 36.0 43 34.423917325184426 36.0 36.0 36.0 32.0 36.0 44 34.35261536992384 36.0 36.0 36.0 32.0 36.0 45 34.31270441903216 36.0 36.0 36.0 32.0 36.0 46 34.29308417090426 36.0 36.0 36.0 32.0 36.0 47 34.264937013074864 36.0 36.0 36.0 32.0 36.0 48 34.21596895615983 36.0 36.0 36.0 32.0 36.0 49 34.18591388736362 36.0 36.0 36.0 32.0 36.0 50 33.59776501858174 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 1.0 18 1.0 19 2.0 20 9.0 21 35.0 22 127.0 23 342.0 24 868.0 25 1915.0 26 3949.0 27 7085.0 28 11501.0 29 17681.0 30 25300.0 31 35742.0 32 51031.0 33 82076.0 34 171620.0 35 596002.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.788176634208654 17.85164286368032 11.611566301497419 26.748614200613613 2 16.209414292535413 20.3004138150565 36.728672608626454 26.76149928378163 3 18.38042270515783 24.090433876097077 28.48032452424034 29.048818894504752 4 12.457723557826215 15.91812495523671 35.473217625197954 36.15093386173913 5 14.480626447346431 36.7129618576965 33.13428589618099 15.67212579877607 6 34.56521912128664 35.28033757490391 16.625384964308733 13.529058339500722 7 30.514201928605395 30.128242112194936 20.7307174276773 18.62683853152237 8 28.153452695598368 32.88513628446404 19.359645553956234 19.60176546598136 9 27.69032278601264 13.923199430207934 18.33272156286961 40.05375622090982 10 15.962402552122242 26.70049468755502 31.55376456930948 25.78333819101326 11 37.60086661732757 20.849448118350164 22.32892464646947 19.220760617852793 12 24.965780814177883 23.588259636082938 28.606741975401977 22.839217574337205 13 29.87060398732301 19.255415871702183 25.056948967756437 25.817031173218368 14 23.654585515550316 19.39619112987859 25.096763604351004 31.852459750220085 15 25.297116623310735 27.20948624464683 22.073424886470463 25.419972245571977 16 25.75832181911688 25.320831331401532 23.82333660605663 25.09751024342496 17 24.19127652971089 25.710144754538007 25.103154518904034 24.995424196847072 18 24.925841704118845 24.677851587912645 26.088102641845772 24.30820406612274 19 25.636136112238482 24.637616285084473 25.34268786656162 24.38355973611542 20 26.10792405307571 23.948328875210017 24.923280157488303 25.020466914225974 21 26.907811492825935 23.902006191260615 24.48900215659592 24.70118015931753 22 25.971389264956173 24.06486903739927 25.012160706345547 24.951580991299004 23 24.736938438919385 23.928803773682503 25.38004412709718 25.954213660300933 24 25.130883281855393 24.552937724127982 25.31501017124497 25.001168822771653 25 25.085574961278635 24.15964442994293 25.421508287913863 25.333272320864573 26 24.77298682487953 24.972245102603587 25.68720728033839 24.56756079217849 27 25.39581163395221 24.547633188189966 24.95401472125808 25.102540456599748 28 24.752644657860756 24.550005272404235 25.53495007132668 25.16239999840833 29 24.76443811327662 24.622086314331874 25.503453846582364 25.110021725809144 30 24.66590267456921 24.794129994051207 25.691522274149808 24.848445057229775 31 25.24158842524338 24.494191332859806 24.801188195244432 25.46303204665238 32 24.9485949280329 24.71462351193881 24.71770731886862 25.619074241159673 33 24.731977561421083 24.453534653160013 25.208384523787025 25.60610326163188 34 25.34682776691264 24.531909348284113 25.32971766339652 24.79154522140673 35 25.714993424547426 24.38597608166692 25.370503375266352 24.5285271185193 36 24.788646922472367 24.887135765377693 25.116545130771296 25.207672181378644 37 25.593436830387784 24.708379839362703 24.891419612711715 24.806763717537798 38 24.82540996327143 24.62714332896941 25.241242643342897 25.30620406441626 39 25.420946764633907 24.509616666368228 24.75373893043809 25.315697638559776 40 25.544959060301874 24.583415816222487 25.36898451045949 24.502640613016148 41 24.704998756528227 25.03327530465058 25.59890574483959 24.662820193981595 42 25.727743430836647 25.130885885811203 24.972121778834435 24.169248904517715 43 24.99756286320799 24.359331448614213 25.286935554145224 25.356170134032574 44 24.78421284566481 24.913133049430353 24.86876699165301 25.433887113251824 45 24.928477068892796 25.136878377195426 24.86123158707295 25.073412966838827 46 24.697794436552748 24.803834966715606 25.037402663547127 25.46096793318452 47 24.955459417087866 24.740092891114067 25.344412357761104 24.960035334036963 48 25.561393700771735 25.154038831513255 24.492820805637514 24.7917466620775 49 24.83186293185686 25.203201890814608 24.70423692480013 25.260698252528396 50 24.820819104140284 25.470495956803603 24.597396318796264 25.111288620259852 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 176.0 1 112.0 2 48.0 3 130.5 4 213.0 5 232.0 6 251.0 7 233.5 8 216.0 9 287.0 10 358.0 11 557.5 12 757.0 13 1303.0 14 1849.0 15 2330.0 16 2811.0 17 2964.5 18 3118.0 19 3056.5 20 2995.0 21 3287.0 22 3579.0 23 3923.0 24 4267.0 25 4940.0 26 5613.0 27 6731.0 28 7849.0 29 9423.0 30 10997.0 31 12700.0 32 14403.0 33 17316.0 34 20229.0 35 23203.5 36 26178.0 37 30737.0 38 35296.0 39 38539.0 40 41782.0 41 45822.0 42 49862.0 43 52188.5 44 54515.0 45 59781.5 46 65048.0 47 68823.5 48 72599.0 49 75767.5 50 78936.0 51 77122.0 52 75308.0 53 74760.5 54 74213.0 55 74225.0 56 74237.0 57 71775.5 58 69314.0 59 63879.0 60 58444.0 61 51737.0 62 45030.0 63 38965.0 64 32900.0 65 28043.5 66 23187.0 67 19748.5 68 16310.0 69 14464.5 70 12619.0 71 9817.5 72 7016.0 73 6005.0 74 4994.0 75 3853.5 76 2713.0 77 2338.0 78 1963.0 79 1637.0 80 1311.0 81 1057.0 82 803.0 83 642.5 84 482.0 85 353.0 86 224.0 87 165.0 88 106.0 89 87.0 90 68.0 91 54.5 92 41.0 93 29.0 94 17.0 95 20.0 96 23.0 97 16.5 98 10.0 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00915160630585464 2 7.957918526830122E-4 3 9.947398158537653E-5 4 0.0 5 0.0 6 0.0 7 1.9894796317075305E-4 8 9.947398158537653E-5 9 8.952658342683888E-4 10 6.963178710976357E-4 11 0.0 12 3.978959263415061E-4 13 1.9894796317075305E-4 14 2.9842194475612955E-4 15 0.004277381208171191 16 8.952658342683888E-4 17 0.0 18 5.968438895122591E-4 19 0.0 20 6.963178710976357E-4 21 0.0 22 9.947398158537653E-5 23 9.947398158537653E-4 24 2.9842194475612955E-4 25 0.002884745465975919 26 0.005968438895122592 27 0.00746054861890324 28 0.005371595005610332 29 0.003183167410732049 30 0.004177907226585814 31 0.0065652827846348506 32 0.0036805373186589315 33 0.005471068987195709 34 0.0033821153739028017 35 0.0033821153739028017 36 0.0097484501953669 37 0.004177907226585814 38 0.007560022600488616 39 0.005371595005610332 40 0.002884745465975919 41 0.003780011300244308 42 0.00228790157646366 43 9.947398158537653E-4 44 0.001492109723780648 45 0.0015915837053660244 46 0.0011936877790245182 47 0.0024868495396344133 48 0.0021884275948782835 49 6.963178710976357E-4 50 0.001890005650122154 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1005288.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.73736306681206 #Duplication Level Percentage of deduplicated Percentage of total 1 81.92448727255581 51.39726302080757 2 11.353156170988624 14.245341613070622 3 3.057056810190968 5.753750490504633 4 1.2370111573870122 3.104272723947455 5 0.6126527625633421 1.9218109399410896 6 0.40810030355936855 1.5361882147248191 7 0.2687545091105063 1.180266444973608 8 0.18955196847743241 0.9513592533118074 9 0.14238350397059793 0.8039489024995451 >10 0.6874547827733174 7.635385601101836 >50 0.05890572109040344 2.5786664443106098 >100 0.05569730407810451 6.859044065287878 >500 0.0047877332545848824 2.0327022855185777 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.947398158537653E-5 2 0.0 0.0 0.0 0.0 9.947398158537653E-5 3 0.0 0.0 0.0 0.0 9.947398158537653E-5 4 0.0 0.0 0.0 0.0 9.947398158537653E-5 5 0.0 0.0 0.0 0.0 9.947398158537653E-5 6 0.0 0.0 0.0 0.0 9.947398158537653E-5 7 0.0 0.0 0.0 0.0 9.947398158537653E-5 8 0.0 0.0 0.0 0.0 9.947398158537653E-5 9 0.0 0.0 0.0 0.0 9.947398158537653E-5 10 0.0 0.0 0.0 0.0 9.947398158537653E-5 11 0.0 0.0 0.0 9.947398158537653E-5 9.947398158537653E-5 12 0.0 0.0 0.0 9.947398158537653E-5 9.947398158537653E-5 13 0.0 0.0 0.0 9.947398158537653E-5 9.947398158537653E-5 14 0.0 0.0 0.0 9.947398158537653E-5 9.947398158537653E-5 15 0.0 0.0 0.0 9.947398158537653E-5 9.947398158537653E-5 16 0.0 0.0 0.0 9.947398158537653E-5 9.947398158537653E-5 17 0.0 0.0 0.0 9.947398158537653E-5 1.9894796317075305E-4 18 0.0 0.0 0.0 9.947398158537653E-5 1.9894796317075305E-4 19 0.0 0.0 0.0 9.947398158537653E-5 1.9894796317075305E-4 20 0.0 0.0 0.0 2.984219447561296E-4 1.9894796317075305E-4 21 0.0 0.0 0.0 4.973699079268827E-4 1.9894796317075305E-4 22 0.0 0.0 0.0 7.957918526830122E-4 1.9894796317075305E-4 23 0.0 0.0 0.0 0.0021884275948782835 1.9894796317075305E-4 24 0.0 0.0 0.0 0.0028847454659759195 1.9894796317075305E-4 25 0.0 0.0 0.0 0.003581063337073555 2.984219447561296E-4 26 0.0 0.0 0.0 0.003978959263415061 2.984219447561296E-4 27 0.0 0.0 0.0 0.005371595005610332 2.984219447561296E-4 28 0.0 0.0 0.0 0.008554762416342381 2.984219447561296E-4 29 0.0 0.0 0.0 0.012931617606098948 2.984219447561296E-4 30 0.0 0.0 0.0 0.023575333635734236 2.984219447561296E-4 31 0.0 0.0 0.0 0.05242278829549343 2.984219447561296E-4 32 0.0 0.0 0.0 0.08345867055013091 2.984219447561296E-4 33 0.0 0.0 0.0 0.11509139669428065 2.984219447561296E-4 34 0.0 0.0 0.0 0.1479178106174549 3.978959263415061E-4 35 0.0 0.0 0.0 0.18681213741733713 3.978959263415061E-4 36 0.0 0.0 0.0 0.24013019154709894 3.978959263415061E-4 37 0.0 0.0 0.0 0.3195104288522294 3.978959263415061E-4 38 0.0 0.0 0.0 0.431418658135778 3.978959263415061E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTCG 265 0.0 22.414715 8 CCTATAC 80 3.615074E-6 21.999626 3 GTATCAA 1775 0.0 21.690853 1 CGCAATA 235 0.0 20.59642 36 GTACTAA 75 5.470081E-5 20.534006 1 GTATAGG 120 1.4302714E-8 20.167328 1 ACGCAAC 55 0.0044822455 20.000656 40 CCGTCGT 275 0.0 20.000656 9 GGTATAT 55 0.0044822455 20.000656 1 GTCGTAG 275 0.0 19.99966 11 ATACCGT 275 0.0 19.99966 6 TACCGTC 285 0.0 19.297918 7 GGCGTTA 240 0.0 19.249674 42 CGGCGTT 245 0.0 18.856823 41 CGTCGTA 305 0.0 18.75378 10 CGAGCCG 335 0.0 18.387747 15 CCTCGCG 180 1.4551915E-11 18.333023 13 TACACCG 60 0.0074119796 18.333023 5 TCCTATA 120 3.1628588E-7 18.332111 2 TAGCGGC 280 0.0 18.07292 30 >>END_MODULE