FastQCFastQC Report
Thu 2 Feb 2017
SRR4063973_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063973_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765123
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64150.3634307637484753No Hit
TATCAACGCAGAGTACTTTTTTTTT44800.2538066752288651No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34260.1940941226192169No Hit
GTCCTAAAGTGTGTATTTCTCATTT28890.16367131355718553No Hit
GTCCTACAGTGGACATTTCTAAATT25420.14401262688209263No Hit
CTGTAGGACGTGGAATATGGCAAGA24890.141010003268894No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG22960.13007592105479335No Hit
GGTATCAACGCAGAGTACTTTTTTT22580.12792309657740566No Hit
GATTAAGAGGGACGGCCGGGGGCAT21940.124297286931279No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21910.12412732710411682No Hit
GCGCAAGACGGACCAGAGCGAAAGC21810.12356079434690953No Hit
CTTTAGGACGTGAAATATGGCGAGG21660.1227109952110986No Hit
GTCTTGCGCCGGTCCAAGAATTTCA21540.12203115590244985No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21380.12112470349091821No Hit
GTATCTGATCGTCTTCGAACCTCCG20580.11659244143325989No Hit
GAATAGGACCGCGGTTCTATTTTGT20540.11636582833037697No Hit
GTACATGGGGTGGTATCAACGCAAA20370.1154027226431246No Hit
GAACTACGACGGTATCTGATCGTCT19840.11240009902992597No Hit
ATCAGATACCGTCGTAGTTCCGACC19780.11206017937560159No Hit
GGGTAGGCACACGCTGAGCCAGTCA18690.10588497232204214No Hit
TCGTAGTTCCGACCATAAACGATGC18520.10492186663478976No Hit
CTATTGGAGCTGGAATTACCGCGGC18500.10480856008334831No Hit
GTCCTACAGTGTGCATTTCTCATTT17690.10021964474996926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT1800.016.3522282
GCGTTAT1800.015.3011491
TCGCGTA1550.014.7121149
GTATACG400.005289572314.2458971
AACCGCG1750.014.1158247
CGCGTAA1700.013.97171410
CGCGCTA1400.013.572906516
ACTCTAA2950.013.20445310
CGAACGA1800.013.19588216
ACGCGCG1450.013.10450513
GTATTAG2600.012.784781
CGCGGTC1600.012.46975510
ATTAGCT2000.012.3460943
AGGCCCG4550.012.31967410
ATAAGAC701.0942879E-412.21042253
GGTCTAG550.003075352912.0874281
AGAACCG2450.012.018655
TATTAGC2450.012.0138832
GCGCGCT1750.011.94449715
GAACCGC2150.011.930186
Produced by FastQC (version 0.11.5)