FastQCFastQC Report
Thu 2 Feb 2017
SRR4063973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765123
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA22100.12520373934281065No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC21590.1223144222810535No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20820.11795212005055737No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG20600.11670574798470135No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20540.11636582833037697No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA20270.11483618988591729No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT19140.10843436972947496No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA18700.10594162559776288No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC18450.10452529370474466No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC18350.10395876094753737No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA17810.10089948405861801No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG508.341334E-526.4010371
TAACGGC550.004483396720.00078636
GTCCTAC17950.019.8559321
GTAGGAC16550.019.5398923
TGTAGGA16500.019.3324492
TCCTACA18350.019.1816842
TAGGACG18500.019.0261574
TTAGGAC11050.018.5149923
CTGTAGG16300.018.4915241
AGGACGT19200.018.103345
GTATCAA54300.017.949141
GGACGTG19200.017.8741846
GTCCTAA10350.017.8557741
TAGGACC14150.017.7235724
GACGTGA10900.017.558837
CTTTAGG11550.017.5244981
CGCAATA5900.017.15321536
CCTACAG19300.017.0976643
ATACGAA5850.016.92230240
TACCGTC6200.016.6766597
Produced by FastQC (version 0.11.5)