##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063973_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1765123
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.279817893710522	32.0	32.0	32.0	32.0	32.0
2	31.447682682736556	32.0	32.0	32.0	32.0	32.0
3	31.51159324307711	32.0	32.0	32.0	32.0	32.0
4	31.588410552692363	32.0	32.0	32.0	32.0	32.0
5	31.534578610102525	32.0	32.0	32.0	32.0	32.0
6	35.13541662535699	36.0	36.0	36.0	36.0	36.0
7	35.15467533990549	36.0	36.0	36.0	36.0	36.0
8	35.109065487221	36.0	36.0	36.0	36.0	36.0
9	35.20687906735111	36.0	36.0	36.0	36.0	36.0
10	35.06615516312461	36.0	36.0	36.0	36.0	36.0
11	35.19485667571042	36.0	36.0	36.0	36.0	36.0
12	35.10352649645379	36.0	36.0	36.0	36.0	36.0
13	35.140143774683125	36.0	36.0	36.0	36.0	36.0
14	35.083040105420416	36.0	36.0	36.0	36.0	36.0
15	35.05971878446998	36.0	36.0	36.0	36.0	36.0
16	35.06452864757867	36.0	36.0	36.0	36.0	36.0
17	35.02725702401476	36.0	36.0	36.0	36.0	36.0
18	35.02479770531572	36.0	36.0	36.0	36.0	36.0
19	35.01968814637847	36.0	36.0	36.0	36.0	36.0
20	35.0127186603993	36.0	36.0	36.0	36.0	36.0
21	34.99890489218032	36.0	36.0	36.0	36.0	36.0
22	34.98712271042868	36.0	36.0	36.0	36.0	36.0
23	34.93959457782829	36.0	36.0	36.0	36.0	36.0
24	34.9020923754322	36.0	36.0	36.0	32.0	36.0
25	34.86603936382904	36.0	36.0	36.0	32.0	36.0
26	34.8034550566731	36.0	36.0	36.0	32.0	36.0
27	34.79823445731544	36.0	36.0	36.0	32.0	36.0
28	34.763149083661595	36.0	36.0	36.0	32.0	36.0
29	34.735334024880984	36.0	36.0	36.0	32.0	36.0
30	34.70041351225949	36.0	36.0	36.0	32.0	36.0
31	34.704471586399364	36.0	36.0	36.0	32.0	36.0
32	34.66133068347079	36.0	36.0	36.0	32.0	36.0
33	34.6339614859701	36.0	36.0	36.0	32.0	36.0
34	34.619551725290535	36.0	36.0	36.0	32.0	36.0
35	34.57144572927779	36.0	36.0	36.0	32.0	36.0
36	34.53982980222908	36.0	36.0	36.0	32.0	36.0
37	34.5150553247564	36.0	36.0	36.0	32.0	36.0
38	34.45990392737503	36.0	36.0	36.0	32.0	36.0
39	34.42489050338135	36.0	36.0	36.0	32.0	36.0
40	34.406116174340255	36.0	36.0	36.0	32.0	36.0
41	34.394756059492735	36.0	36.0	36.0	32.0	36.0
42	34.31185815379438	36.0	36.0	36.0	32.0	36.0
43	34.3091676897304	36.0	36.0	36.0	32.0	36.0
44	34.21943626591462	36.0	36.0	36.0	32.0	36.0
45	34.17524841045071	36.0	36.0	36.0	32.0	36.0
46	34.12426726069515	36.0	36.0	36.0	32.0	36.0
47	34.083736374179026	36.0	36.0	36.0	32.0	36.0
48	34.031470328130105	36.0	36.0	36.0	32.0	36.0
49	33.99775653028146	36.0	36.0	36.0	32.0	36.0
50	33.41535858974134	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	3.0
19	3.0
20	16.0
21	58.0
22	205.0
23	594.0
24	1634.0
25	3688.0
26	7535.0
27	13230.0
28	21687.0
29	32846.0
30	46526.0
31	64813.0
32	92459.0
33	146167.0
34	301601.0
35	1032056.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.013983359349105	17.651611514174583	11.73338697020604	26.60101815627027
2	15.914212906361854	20.04782693922319	37.584654588882074	26.453305565532876
3	18.836099157222527	24.025219872235027	28.031812073540774	29.106868897001668
4	12.415282107819117	15.692844068090439	34.976882630842155	36.914991193248284
5	14.44596212275292	36.77177171222629	32.70310340979071	16.07916275523009
6	34.93806079820159	34.8143863469751	16.505752023377475	13.74180083144583
7	30.795979876722264	29.809814630166787	20.350061185641767	19.04414430746918
8	28.307262440067916	33.28589565712984	18.79851998982507	19.60832191297717
9	27.905107169140454	13.755256763470996	18.198783416529423	40.140852650859124
10	16.883575264035134	26.129422255660568	30.494089306817834	26.492913173486464
11	37.876057362574734	20.27932331061348	22.70289379267054	19.141725534141248
12	26.255140582430325	22.83594155868068	28.35692283985064	22.551995019038355
13	30.45210626383762	19.117723707677005	24.86318179655048	25.5669882319349
14	23.829398374499608	19.889321847037987	24.47213160819843	31.809148170263974
15	25.062746240041335	28.351555710162796	21.667480620901667	24.918217428894206
16	25.62139433222877	24.96760812258548	24.670061747104842	24.740935798080905
17	23.95980336781063	25.934793212710954	25.16544172842346	24.939961691054958
18	25.00835638183963	23.819937250654064	27.433350064981493	23.738356302524817
19	26.296595929346527	23.67207479143085	25.145797287666234	24.88553199155639
20	26.703106260052472	23.454419065521584	25.132555252836504	24.709919421589436
21	27.254644577176773	23.26353460920287	24.150838213540926	25.330982600079427
22	26.638555295328025	24.351857831923233	24.647814711957587	24.36177216079115
23	24.268670397885227	24.162557850600557	25.93310207256557	25.63566967894864
24	25.664661879046495	24.12299983910333	25.634295465206662	24.578042816643514
25	25.09632626005966	24.276087122789423	25.67340568071407	24.95418093643685
26	24.180919509492202	25.578857672512846	26.194485299949914	24.04573751804504
27	25.12338436034182	24.582028574293787	25.285649454139996	25.00893761122439
28	24.21657715987656	24.83746854892487	25.575580878199567	25.370373412999005
29	24.426045519775602	24.30775053127934	25.532341611905192	25.73386233703987
30	23.912553284549123	24.74850599302457	26.2810387456007	25.057901976825615
31	25.638534147695722	24.239198177883036	24.726739113191083	25.395528561230158
32	24.853688853838424	24.870458688984147	24.69324232298476	25.582610134192674
33	23.956119040388703	24.636327170705837	25.949889181484014	25.457664607421442
34	25.22537222108913	24.662224941645704	25.75639631064881	24.35600652661636
35	25.43583183245112	24.60986032872295	25.90187487996247	24.052432958863456
36	23.97269315952201	25.230271861591554	25.6540729379783	25.142962040908134
37	25.308800750571937	24.95351417010471	24.621966240126365	25.115718839196987
38	24.609907057594377	25.207879594144693	25.059151348129483	25.123062000131448
39	25.819343573322094	24.247198790283058	24.67897346293939	25.25448417345546
40	26.1379802263808	24.5187325893062	25.354445891656972	23.988841292656023
41	24.862965852365146	24.688355312619155	26.1594009532761	24.2892778817396
42	25.93676791769235	25.691057371174757	25.136069934309113	23.23610477682378
43	24.70823116703001	24.706078309621688	25.54019724706192	25.04549327628639
44	24.082216537920058	25.47732558304227	24.938091150901226	25.502366728136444
45	24.3789020452099	25.78295847260779	24.925103393575434	24.913036088606876
46	24.28682551310095	24.74306002885954	25.16524550153221	25.804868956507303
47	25.385534933260818	24.490561334330454	25.413692297232988	24.710211435175744
48	25.312874982578716	25.471564493887538	24.135082676045645	25.080477847488105
49	24.916719165696	25.38807735713088	24.40224756276089	25.29295591441223
50	24.5952349387966	26.292081232883387	24.172595515943023	24.94008831237699
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	128.0
1	87.0
2	46.0
3	165.5
4	285.0
5	344.0
6	403.0
7	374.0
8	345.0
9	479.0
10	613.0
11	962.5
12	1312.0
13	2392.5
14	3473.0
15	4696.0
16	5919.0
17	6379.5
18	6840.0
19	6857.5
20	6875.0
21	7115.5
22	7356.0
23	7834.0
24	8312.0
25	8237.0
26	8162.0
27	10704.5
28	13247.0
29	16886.5
30	20526.0
31	25528.5
32	30531.0
33	38173.5
34	45816.0
35	54175.5
36	62535.0
37	68174.5
38	73814.0
39	74153.5
40	74493.0
41	76766.0
42	79039.0
43	80346.5
44	81654.0
45	90464.0
46	99274.0
47	107686.0
48	116098.0
49	122351.0
50	128604.0
51	124455.5
52	120307.0
53	120928.0
54	121549.0
55	124555.5
56	127562.0
57	125716.5
58	123871.0
59	114820.0
60	105769.0
61	94425.0
62	83081.0
63	73063.5
64	63046.0
65	53914.0
66	44782.0
67	38957.0
68	33132.0
69	29753.0
70	26374.0
71	20090.0
72	13806.0
73	11832.5
74	9859.0
75	7656.5
76	5454.0
77	4763.0
78	4072.0
79	3404.0
80	2736.0
81	2244.5
82	1753.0
83	1468.5
84	1184.0
85	862.0
86	540.0
87	382.5
88	225.0
89	169.0
90	113.0
91	98.5
92	84.0
93	68.0
94	52.0
95	45.5
96	39.0
97	26.0
98	13.0
99	16.5
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00951775032108244
2	5.098794814865593E-4
3	3.965729300451017E-4
4	0.0
5	0.0
6	2.8326637860364405E-4
7	1.6995982716218642E-4
8	0.0
9	9.06452411531661E-4
10	9.631056872523899E-4
11	0.0
12	1.1330655144145763E-4
13	3.965729300451017E-4
14	2.8326637860364405E-4
15	0.004362302230496118
16	0.0011330655144145762
17	0.0
18	2.8326637860364405E-4
19	5.6653275720728815E-5
20	5.665327572072881E-4
21	0.0
22	5.6653275720728815E-5
23	9.06452411531661E-4
24	8.497991358109322E-4
25	0.0015862917201804067
26	0.005382061193469237
27	0.00623186032928017
28	0.004532262057658305
29	0.0024927441317120673
30	0.004022382576171745
31	0.00810141842806422
32	0.0027760105103157118
33	0.004248995679054661
34	0.0024360908559913387
35	0.0028326637860364404
36	0.008158071703784948
37	0.003909076024730288
38	0.00900787083959588
39	0.004645568609099762
40	0.0024927441317120673
41	0.002945970337477898
42	0.0021528244773876947
43	0.001529638444459678
44	0.0015862917201804067
45	0.0013030253415767625
46	0.0012463720658560337
47	0.0024360908559913387
48	0.002322784304549881
49	6.231860329280168E-4
50	0.0014163318930182202
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1765123.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.99977250648965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.4808762776757	34.79065572399456
2	15.25897517240107	14.648546739148502
3	5.414872930777747	7.79738006486642
4	2.451735967115107	4.70731074670014
5	1.3013327079122672	3.123183696752148
6	0.7787886035548017	2.242900548076634
7	0.48137546730564135	1.6174139044613152
8	0.33320151229911915	1.2794877431340823
9	0.2396669025639369	1.0353561120363592
>10	1.0773996088110627	9.025506667201665
>50	0.07891159604916571	2.70126987112383
>100	0.08383700105392662	8.781719860485907
>500	0.01367511897027717	4.5338012944906145
>1k	0.005351133510108457	3.7154670275279433
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2210	0.12520373934281065	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2159	0.1223144222810535	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2082	0.11795212005055737	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2060	0.11670574798470135	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2054	0.11636582833037697	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2027	0.11483618988591729	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1914	0.10843436972947496	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1870	0.10594162559776288	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1845	0.10452529370474466	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1835	0.10395876094753737	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1781	0.10089948405861801	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.665327572072881E-5
2	0.0	0.0	0.0	0.0	5.665327572072881E-5
3	0.0	0.0	0.0	0.0	5.665327572072881E-5
4	0.0	0.0	0.0	0.0	5.665327572072881E-5
5	0.0	0.0	0.0	0.0	1.1330655144145762E-4
6	0.0	0.0	0.0	0.0	1.6995982716218642E-4
7	0.0	0.0	0.0	0.0	1.6995982716218642E-4
8	0.0	0.0	0.0	0.0	2.2661310288291523E-4
9	0.0	0.0	0.0	0.0	2.2661310288291523E-4
10	0.0	0.0	0.0	0.0	2.2661310288291523E-4
11	0.0	0.0	0.0	0.0	2.2661310288291523E-4
12	0.0	0.0	0.0	0.0	6.231860329280169E-4
13	0.0	0.0	0.0	0.0	8.497991358109322E-4
14	0.0	0.0	0.0	0.0	8.497991358109322E-4
15	0.0	0.0	0.0	5.665327572072881E-5	0.0010197589629731185
16	0.0	0.0	0.0	1.1330655144145762E-4	0.0010197589629731185
17	0.0	0.0	0.0	2.2661310288291523E-4	0.0010197589629731185
18	0.0	0.0	0.0	2.8326637860364405E-4	0.0011330655144145762
19	0.0	0.0	0.0	3.3991965432437284E-4	0.0011330655144145762
20	0.0	0.0	0.0	3.3991965432437284E-4	0.0013030253415767627
21	0.0	0.0	0.0	6.231860329280169E-4	0.0014163318930182202
22	0.0	0.0	0.0	6.798393086487457E-4	0.0014163318930182202
23	0.0	0.0	0.0	0.001756251547342593	0.0014163318930182202
24	0.0	0.0	0.0	0.003512503094685186	0.0014163318930182202
25	0.0	0.0	0.0	0.004022382576171745	0.0014163318930182202
26	0.0	0.0	0.0	0.005155448090586321	0.0016995982716218644
27	0.0	0.0	0.0	0.005948593950676525	0.0016995982716218644
28	0.0	0.0	0.0	0.00957440359680317	0.001756251547342593
29	0.0	0.0	0.0	0.015409690996038236	0.0018695580987840507
30	0.0	0.0	0.0	0.030536115613472827	0.0018695580987840507
31	0.0	0.0	0.0	0.06747405138338801	0.0018695580987840507
32	0.0	0.0	0.0	0.10384545439609591	0.0018695580987840507
33	0.0	0.0	0.0	0.1363077813840735	0.0018695580987840507
34	0.0	0.0	0.0	0.1687134550963304	0.002096171201666966
35	0.0	0.0	0.0	0.21086349223255263	0.002096171201666966
36	0.0	0.0	0.0	0.2725589094924263	0.002096171201666966
37	0.0	0.0	0.0	0.3613912458225291	0.002096171201666966
38	0.0	0.0	0.0	0.47713388811997803	0.0022094777531084238
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTACG	50	8.341334E-5	26.401037	1
TAACGGC	55	0.0044833967	20.000786	36
GTCCTAC	1795	0.0	19.855932	1
GTAGGAC	1655	0.0	19.539892	3
TGTAGGA	1650	0.0	19.332449	2
TCCTACA	1835	0.0	19.181684	2
TAGGACG	1850	0.0	19.026157	4
TTAGGAC	1105	0.0	18.514992	3
CTGTAGG	1630	0.0	18.491524	1
AGGACGT	1920	0.0	18.10334	5
GTATCAA	5430	0.0	17.94914	1
GGACGTG	1920	0.0	17.874184	6
GTCCTAA	1035	0.0	17.855774	1
TAGGACC	1415	0.0	17.723572	4
GACGTGA	1090	0.0	17.55883	7
CTTTAGG	1155	0.0	17.524498	1
CGCAATA	590	0.0	17.153215	36
CCTACAG	1930	0.0	17.097664	3
ATACGAA	585	0.0	16.922302	40
TACCGTC	620	0.0	16.676659	7
>>END_MODULE