FastQCFastQC Report
Thu 2 Feb 2017
SRR4063972_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063972_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5842740
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT91380.15639922365191675No Hit
TATCAACGCAGAGTACTTTTTTTTT61170.10469403054046561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62800.013.5659091
CGGTTCT11200.012.30027712
AACCGCG4500.012.2449797
GGTATCA20800.011.9177241
CGGACAT6700.011.7667525
CGTTATT4150.011.4412862
TTTACGC752.0821136E-411.3962033
CGAACGA5450.011.15788516
GGTTCTA12350.011.07750213
CGTCGTA8800.011.01175110
CGGTCCA9400.010.915277510
TAGAACC6550.010.8758244
CGCATCG7000.010.85771113
TAGACCG1053.4776895E-610.8553865
CCGTCGT8500.010.8417439
AAGACGG10050.010.7743755
CAAGACG10650.010.7022194
CGTCTTA2800.010.51966915
ACGAACG5700.010.50180815
GCGTTAT4350.010.48016551
Produced by FastQC (version 0.11.5)