##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063972_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5842740
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.231476841344985	32.0	32.0	32.0	32.0	32.0
2	30.72972714856386	32.0	32.0	32.0	32.0	32.0
3	30.751794877061105	32.0	32.0	32.0	32.0	32.0
4	30.793132502901035	32.0	32.0	32.0	32.0	32.0
5	30.655188661484168	32.0	32.0	32.0	32.0	32.0
6	34.33515987362094	36.0	36.0	36.0	32.0	36.0
7	34.27296850450303	36.0	36.0	36.0	32.0	36.0
8	34.214695160147464	36.0	36.0	36.0	32.0	36.0
9	34.40803544227537	36.0	36.0	36.0	32.0	36.0
10	34.02158251094521	36.0	36.0	36.0	32.0	36.0
11	34.386428627664415	36.0	36.0	36.0	32.0	36.0
12	34.147402246206404	36.0	36.0	36.0	32.0	36.0
13	34.260393753615595	36.0	36.0	36.0	32.0	36.0
14	34.13436949102647	36.0	36.0	36.0	32.0	36.0
15	34.05095195062591	36.0	36.0	36.0	32.0	36.0
16	34.05624809592759	36.0	36.0	36.0	32.0	36.0
17	33.975067006233374	36.0	36.0	36.0	32.0	36.0
18	33.97140913338605	36.0	36.0	36.0	32.0	36.0
19	34.00185238432653	36.0	36.0	36.0	32.0	36.0
20	33.98240517291545	36.0	36.0	36.0	32.0	36.0
21	33.97506563701277	36.0	36.0	36.0	32.0	36.0
22	33.94519848564201	36.0	36.0	36.0	32.0	36.0
23	33.876290233691726	36.0	36.0	36.0	32.0	36.0
24	33.858891547458896	36.0	36.0	36.0	32.0	36.0
25	33.4316462823949	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	13.0
4	62.0
5	79.0
6	250.0
7	108.0
8	315.0
9	376.0
10	530.0
11	155.0
12	341.0
13	343.0
14	491.0
15	866.0
16	1561.0
17	2045.0
18	2795.0
19	3827.0
20	6185.0
21	9609.0
22	15241.0
23	23902.0
24	35395.0
25	51649.0
26	72483.0
27	95635.0
28	128325.0
29	171725.0
30	224611.0
31	303038.0
32	425527.0
33	596621.0
34	1238137.0
35	2430499.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.66184378209851	16.890397760845584	11.03318100978714	26.414577447268766
2	17.122097625724976	19.279989524879696	36.76442019267717	26.833492656718157
3	18.83291962131444	23.03972302888887	28.351319807805766	29.77603754199093
4	12.719543197817245	15.230955635821406	35.39990986779869	36.64959129856266
5	14.900990421011947	36.06503893592817	33.595730484707545	15.438240158352338
6	35.02752510296237	35.035433434783656	16.530433383430847	13.406608078823131
7	30.95893600302669	30.129130666264935	20.419730302993987	18.49220302771439
8	28.54303860189563	32.59661602912306	18.989934927851518	19.870410441129792
9	27.291481050783823	14.177779931583192	18.256554641577193	40.27418437605579
10	15.709481328951993	26.630878179400707	31.510446100805094	26.1491943908422
11	37.32789815828189	20.910325605322242	22.307022202098707	19.454754034297157
12	24.941191944867803	23.51109650412551	28.404046576493457	23.143664974513225
13	29.478731491990516	19.38498252493496	24.948025232873846	26.18826075020068
14	23.841642715930426	19.35862323272511	24.948768745757846	31.850965305586616
15	25.525427502290572	26.8886224878621	21.89066901860716	25.695280991240168
16	26.156305057812894	25.63095279485383	23.19448909346848	25.018253053864793
17	24.44361020465554	25.689923346227133	24.80473479794937	25.06173165116796
18	25.021753354641348	24.94421206608909	25.46559030382756	24.568444275442005
19	25.717664380504374	24.78112688012327	25.068213186204098	24.43299555316826
20	25.96620134521106	24.317155594777223	24.423167146936052	25.293475913075664
21	26.92413096635267	24.172159681442825	24.26740961961001	24.63629973259449
22	26.24319532394292	24.0950236901475	24.514630749384946	25.14715023652464
23	24.737270975540863	24.09724181416509	25.107201116025585	26.058286094268464
24	25.006316183973183	24.78933064208228	24.82528364590522	25.379069528039317
25	25.064156080756327	24.362079071534502	24.943397738924233	25.63036710878494
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	251.0
1	251.0
2	704.0
3	1157.0
4	1157.0
5	1157.0
6	3121.0
7	5085.0
8	5085.0
9	5085.0
10	6463.0
11	7841.0
12	7841.0
13	7841.0
14	11080.0
15	14319.0
16	14319.0
17	14319.0
18	23893.0
19	33467.0
20	33467.0
21	33467.0
22	53332.0
23	73197.0
24	73197.0
25	73197.0
26	110301.0
27	147405.0
28	147405.0
29	147405.0
30	184535.5
31	221666.0
32	221666.0
33	221666.0
34	283487.0
35	345308.0
36	345308.0
37	345308.0
38	405142.5
39	464977.0
40	464977.0
41	464977.0
42	537109.5
43	609242.0
44	609242.0
45	609242.0
46	681149.0
47	753056.0
48	753056.0
49	753056.0
50	777771.0
51	802486.0
52	802486.0
53	802486.0
54	759793.0
55	717100.0
56	717100.0
57	717100.0
58	659888.0
59	602676.0
60	602676.0
61	602676.0
62	527655.0
63	452634.0
64	452634.0
65	452634.0
66	371293.5
67	289953.0
68	289953.0
69	289953.0
70	218612.5
71	147272.0
72	147272.0
73	147272.0
74	111398.0
75	75524.0
76	75524.0
77	75524.0
78	59899.5
79	44275.0
80	44275.0
81	44275.0
82	30522.5
83	16770.0
84	16770.0
85	16770.0
86	12866.0
87	8962.0
88	8962.0
89	8962.0
90	6269.5
91	3577.0
92	3577.0
93	3577.0
94	2507.5
95	1438.0
96	1438.0
97	1438.0
98	2270.0
99	3102.0
100	3102.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03972451281419334
2	0.005562458709441118
3	0.0018655630748587136
4	0.0036113193467448494
5	0.009892618874021436
6	0.013692206054008907
7	0.023447902867490253
8	0.03672934273987889
9	0.050438664051455306
10	0.05873956397169821
11	0.05557324132170865
12	0.06553432122600013
13	0.06587662637735035
14	0.06878622016382725
15	0.06144377466736497
16	0.06830699295193694
17	0.06431913793870685
18	0.07804557450785077
19	0.07575212999380428
20	0.0803903647945998
21	0.07558097741812916
22	0.07973998500703437
23	0.08353957218702183
24	0.0780969202805533
25	0.0793121035678466
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5842740.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.961212824397535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.00394530461307	22.37655553395677
2	15.99300626126422	11.182697912039615
3	7.165922979055075	7.5158807506195595
4	3.8107817436486418	5.329182062681156
5	2.261652340319777	3.9535054402358245
6	1.4797369606263893	3.1040039282751817
7	0.9825854073731816	2.4046664281724808
8	0.7167267267027027	2.00461085033496
9	0.5218549540447898	1.6420213900643479
>10	2.7349398203279636	17.121867793864553
>50	0.18749881402366572	4.520334124397233
>100	0.11692717914880614	8.040492308009455
>500	0.013343212400334332	3.24110558125656
>1k	0.010881348958975919	7.120887596224882
>5k	1.9694749246878873E-4	0.44218829986757147
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	9138	0.15639922365191675	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6117	0.10469403054046561	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3692206054008907E-4
2	0.0	0.0	0.0	0.0	1.3692206054008907E-4
3	0.0	0.0	0.0	0.0	1.3692206054008907E-4
4	0.0	0.0	0.0	0.0	1.3692206054008907E-4
5	0.0	0.0	0.0	0.0	1.540373181076002E-4
6	0.0	0.0	0.0	0.0	2.053830908101336E-4
7	0.0	0.0	0.0	0.0	2.053830908101336E-4
8	0.0	0.0	0.0	0.0	2.053830908101336E-4
9	0.0	0.0	0.0	3.423051513502227E-5	2.2249834837764472E-4
10	0.0	0.0	0.0	5.13457727025334E-5	2.3961360594515588E-4
11	0.0	0.0	0.0	6.846103027004453E-5	2.3961360594515588E-4
12	0.0	0.0	0.0	6.846103027004453E-5	3.765356664852449E-4
13	0.0	0.0	0.0	6.846103027004453E-5	4.4499669675528944E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6280	0.0	13.565909	1
CGGTTCT	1120	0.0	12.300277	12
AACCGCG	450	0.0	12.244979	7
GGTATCA	2080	0.0	11.917724	1
CGGACAT	670	0.0	11.766752	5
CGTTATT	415	0.0	11.441286	2
TTTACGC	75	2.0821136E-4	11.396203	3
CGAACGA	545	0.0	11.157885	16
GGTTCTA	1235	0.0	11.077502	13
CGTCGTA	880	0.0	11.011751	10
CGGTCCA	940	0.0	10.9152775	10
TAGAACC	655	0.0	10.875824	4
CGCATCG	700	0.0	10.857711	13
TAGACCG	105	3.4776895E-6	10.855386	5
CCGTCGT	850	0.0	10.841743	9
AAGACGG	1005	0.0	10.774375	5
CAAGACG	1065	0.0	10.702219	4
CGTCTTA	280	0.0	10.519669	15
ACGAACG	570	0.0	10.501808	15
GCGTTAT	435	0.0	10.4801655	1
>>END_MODULE