FastQCFastQC Report
Thu 2 Feb 2017
SRR4063972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5842740
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70350.027.5199571
GGTATCA31200.021.1541631
TCAACGC102050.018.6893584
ATACCGT12850.018.3177746
AACGCAG104500.018.2723926
ATCAACG104400.018.268673
CAACGCA104750.018.1866265
TATCAAC107800.017.9576072
CGTCGTA13100.017.80103110
TACCGTC13050.017.1943347
ACCGTCG13000.017.0913948
ATACGAA16000.016.49982340
CGCAATA16300.016.19753336
CCGTCGT13800.015.9417229
CGAATGC16900.015.36038943
CAATACG17450.015.25537338
ACGCAGA125950.015.1431657
TACGAAT17450.015.12890741
CGGATCG3650.015.06962226
GTCGTAG15050.014.90988411
Produced by FastQC (version 0.11.5)