##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063972_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5842740
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.345678739769355	32.0	32.0	32.0	32.0	32.0
2	31.414964725454155	32.0	32.0	32.0	32.0	32.0
3	31.48324878396095	32.0	32.0	32.0	32.0	32.0
4	31.563947223391764	32.0	32.0	32.0	32.0	32.0
5	31.517144695810526	32.0	32.0	32.0	32.0	32.0
6	35.10643413877735	36.0	36.0	36.0	36.0	36.0
7	35.11814302878444	36.0	36.0	36.0	36.0	36.0
8	35.06645169903162	36.0	36.0	36.0	36.0	36.0
9	35.17103225541441	36.0	36.0	36.0	36.0	36.0
10	35.03006791334203	36.0	36.0	36.0	36.0	36.0
11	35.16965738677401	36.0	36.0	36.0	36.0	36.0
12	35.077405463874825	36.0	36.0	36.0	36.0	36.0
13	35.11852024906123	36.0	36.0	36.0	36.0	36.0
14	35.058714233390496	36.0	36.0	36.0	36.0	36.0
15	35.03569883308174	36.0	36.0	36.0	36.0	36.0
16	35.04700808182462	36.0	36.0	36.0	36.0	36.0
17	35.010749750973005	36.0	36.0	36.0	36.0	36.0
18	35.00680981868096	36.0	36.0	36.0	36.0	36.0
19	35.01110249643147	36.0	36.0	36.0	36.0	36.0
20	35.00199392750662	36.0	36.0	36.0	36.0	36.0
21	35.00109417841629	36.0	36.0	36.0	36.0	36.0
22	34.983588008365935	36.0	36.0	36.0	36.0	36.0
23	34.93308156789452	36.0	36.0	36.0	36.0	36.0
24	34.89978982463707	36.0	36.0	36.0	32.0	36.0
25	34.87548461851802	36.0	36.0	36.0	32.0	36.0
26	34.815073065034554	36.0	36.0	36.0	32.0	36.0
27	34.8137168520249	36.0	36.0	36.0	32.0	36.0
28	34.79228991877099	36.0	36.0	36.0	32.0	36.0
29	34.76431742641295	36.0	36.0	36.0	32.0	36.0
30	34.74829617610916	36.0	36.0	36.0	32.0	36.0
31	34.747270801028286	36.0	36.0	36.0	32.0	36.0
32	34.70755450353772	36.0	36.0	36.0	32.0	36.0
33	34.689163988128854	36.0	36.0	36.0	32.0	36.0
34	34.68291400952293	36.0	36.0	36.0	32.0	36.0
35	34.64516048977021	36.0	36.0	36.0	32.0	36.0
36	34.626015876112916	36.0	36.0	36.0	32.0	36.0
37	34.62321667573775	36.0	36.0	36.0	32.0	36.0
38	34.58529645337633	36.0	36.0	36.0	32.0	36.0
39	34.587198129644655	36.0	36.0	36.0	32.0	36.0
40	34.56977548205123	36.0	36.0	36.0	32.0	36.0
41	34.55404707380441	36.0	36.0	36.0	32.0	36.0
42	34.49224387872813	36.0	36.0	36.0	32.0	36.0
43	34.486396793285344	36.0	36.0	36.0	32.0	36.0
44	34.43210634051832	36.0	36.0	36.0	32.0	36.0
45	34.40648651146551	36.0	36.0	36.0	32.0	36.0
46	34.391576041377846	36.0	36.0	36.0	32.0	36.0
47	34.37127306708839	36.0	36.0	36.0	32.0	36.0
48	34.33284126967827	36.0	36.0	36.0	32.0	36.0
49	34.31869670736675	36.0	36.0	36.0	32.0	36.0
50	33.72098946727049	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	4.0
18	7.0
19	16.0
20	66.0
21	229.0
22	681.0
23	1979.0
24	4902.0
25	11114.0
26	22279.0
27	40297.0
28	65474.0
29	100291.0
30	142514.0
31	202682.0
32	291185.0
33	468566.0
34	984601.0
35	3505852.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.64141245308087	17.740853540076202	11.577816221982246	27.039917784860684
2	16.2753449976475	19.952587159304713	36.345240513794856	27.42682732925293
3	18.039569604346745	23.558423247266642	28.689734587521524	29.712272560865088
4	12.325073510031253	15.818982189862977	35.4648503955336	36.391093904572166
5	14.472387954966335	36.47976805402944	33.423804584835196	15.624039406169024
6	35.02982338735489	35.38099484316407	16.334384782456148	13.2547969870249
7	30.815255044896773	30.275747526501284	20.456118503386083	18.452878925215856
8	28.475993878898237	32.771821572040096	18.85743997806508	19.89474457099658
9	27.291048584945866	13.973413543182453	18.126785783358784	40.608752088512894
10	15.557395270058642	26.805083408441384	31.589759792314982	26.047761529185
11	37.49722518839195	20.815614046768133	22.260604144734174	19.426556620105742
12	24.8650845879541	23.528244905853164	28.394133911601333	23.212536594591406
13	29.500627533818623	19.319518175888494	25.011343189371104	26.168511100921783
14	23.759789960840155	19.25194087685188	25.009978229317852	31.978290932990117
15	25.49209058975218	26.954879194094417	21.83790043630512	25.71512977984829
16	26.188524625236514	25.623989655440948	23.14632741624784	25.041158303074702
17	24.45587591709984	25.70284245111753	24.761999466346175	25.07928216543645
18	25.066873830488852	24.907701363012666	25.50646368582846	24.518961120670017
19	25.728280630266585	24.76133604576214	25.09236258231164	24.41802074165964
20	26.007177172299546	24.250537979845248	24.44258989564911	25.299694952206096
21	26.915351359122603	24.206074547215863	24.23489664095955	24.643677452701986
22	26.200157734429713	24.040192156682867	24.607443914935683	25.15220619395173
23	24.72479728097985	24.000336830978455	25.16130832135113	26.113557566690567
24	25.031945814148443	24.683973487627135	24.886259893960094	25.397820804264327
25	25.108577423940176	24.229585946679606	25.06577117569988	25.596065453680332
26	24.998237027086795	25.000718882158786	25.116441793480902	24.88460229727352
27	25.571767597539697	24.63533175349366	24.51662982371009	25.276270825256553
28	25.09238355564152	24.326169150482073	25.3039029591835	25.277544334692912
29	24.98034258164803	24.650351266170517	25.115197435104847	25.254108717076605
30	24.962670064301285	24.680239404528475	25.29242638762611	25.064664143544128
31	25.291266547981124	24.60688194725083	24.551869639659117	25.549981865108933
32	25.316701816771474	24.593457459244764	24.339288141058667	25.750552582925096
33	24.886760261752215	24.293466874980314	24.869815379517437	25.94995748375003
34	25.451624265863458	24.42613517525996	25.087059171482178	25.035181387394402
35	25.873666381289578	24.160445366262266	25.14266857587476	24.823219676573395
36	24.939526868351038	24.685036485855484	24.826933531247477	25.548503114546005
37	25.80501387417869	24.550467025462353	24.642345376334955	25.002173724023997
38	25.087410208444272	24.331900502560558	25.018687050485934	25.56200223850924
39	25.542443767602713	24.497325200962944	24.457975573791675	25.50225545764267
40	25.79310356631525	24.484521834085427	24.880374012530414	24.842000587068906
41	24.817993994721803	24.89296155584732	25.160517016010363	25.128527433420505
42	25.896952359370406	24.718696310455346	24.85005865521244	24.534292674961808
43	25.321946736922353	23.922600993828325	25.065094505772116	25.690357763477213
44	24.953766785373993	24.50316730681448	24.834146632844178	25.708919274967347
45	25.033708949790352	24.653283041189102	24.901389127079405	25.411618881941145
46	24.944772766054747	24.591664200617558	24.85736423308554	25.606198800242147
47	25.123668960502954	24.45100771675144	25.265386349329255	25.159936973416354
48	25.736405502821256	24.937172695115517	24.394520729887002	24.93190107217622
49	24.978943857074615	24.909387128890295	24.655960338972665	25.45570867506242
50	25.072064951393596	25.03435943235308	24.653401900440315	25.240173715813008
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	61.0
1	79.5
2	98.0
3	294.0
4	490.0
5	605.0
6	720.0
7	677.0
8	634.0
9	852.5
10	1071.0
11	1646.5
12	2222.0
13	4023.0
14	5824.0
15	7595.0
16	9366.0
17	9857.5
18	10349.0
19	10380.5
20	10412.0
21	12613.0
22	14814.0
23	17533.5
24	20253.0
25	25875.0
26	31497.0
27	38712.5
28	45928.0
29	53833.5
30	61739.0
31	70867.5
32	79996.0
33	91325.5
34	102655.0
35	121599.0
36	140543.0
37	168506.5
38	196470.0
39	220453.0
40	244436.0
41	271225.5
42	298015.0
43	310483.0
44	322951.0
45	355197.0
46	387443.0
47	411647.0
48	435851.0
49	450861.0
50	465871.0
51	458269.5
52	450668.0
53	446441.0
54	442214.0
55	441411.0
56	440608.0
57	427653.0
58	414698.0
59	380371.5
60	346045.0
61	302391.0
62	258737.0
63	224096.0
64	189455.0
65	160910.5
66	132366.0
67	113001.5
68	93637.0
69	83339.5
70	73042.0
71	56148.0
72	39254.0
73	33701.5
74	28149.0
75	21854.0
76	15559.0
77	13447.5
78	11336.0
79	9474.0
80	7612.0
81	6170.5
82	4729.0
83	3694.5
84	2660.0
85	1886.0
86	1112.0
87	777.0
88	442.0
89	345.0
90	248.0
91	208.5
92	169.0
93	132.0
94	95.0
95	101.5
96	108.0
97	77.5
98	47.0
99	44.0
100	41.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008677435586728144
2	3.2518989378271156E-4
3	3.936509240527561E-4
4	0.0
5	0.0
6	1.7115257567511133E-4
7	6.846103027004453E-5
8	1.7115257567511134E-5
9	0.001129606999455735
10	7.873018481055122E-4
11	1.7115257567511134E-5
12	8.557628783755567E-5
13	4.621119543228006E-4
14	3.4230515135022266E-4
15	0.004604004285660495
16	9.413391662131124E-4
17	1.7115257567511134E-5
18	3.594204089177338E-4
19	1.0269154540506681E-4
20	8.386476208080455E-4
21	0.0
22	1.3692206054008907E-4
23	8.044171056730233E-4
24	5.819187572953786E-4
25	0.002310559771614003
26	0.005750726542683741
27	0.006418221587816675
28	0.004278814391877783
29	0.0027726717259368037
30	0.004176122846472717
31	0.007034370860247076
32	0.003046515847016982
33	0.004586889028092984
34	0.0027726717259368037
35	0.003046515847016982
36	0.0081468626021353
37	0.003970739755662583
38	0.007855903223487611
39	0.004193238104040228
40	0.0026528649229642254
41	0.003645549861879871
42	0.0020880614232363583
43	0.0013863358629684019
44	0.0013521053478333797
45	0.0013349900902658684
46	0.0010440307116181791
47	0.0021565224535064027
48	0.002344790286749025
49	4.621119543228006E-4
50	0.0016088342113460465
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5842740.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.18361753109776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.84035292922941	24.10998883901861
2	16.06908954880065	11.950137597111276
3	6.972566778676984	7.7779576892509015
4	3.662807659263251	5.447857563680807
5	2.163156408396628	4.021699027488166
6	1.3864028987871095	3.0930885079503074
7	0.9176756331308416	2.388574983196164
8	0.6835306588679759	2.0332914072100863
9	0.49978670717492396	1.6725489990047213
>10	2.5185296684306095	16.698216382563913
>50	0.16566623870300884	4.246857347375453
>100	0.0999597019032673	7.243340984373497
>500	0.011607248799424217	3.013460863426053
>1k	0.008821490936585784	6.2129483379099835
>5k	4.642889966561137E-5	0.09003147044003527
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.13457727025334E-5
2	0.0	0.0	0.0	0.0	5.13457727025334E-5
3	0.0	0.0	0.0	0.0	5.13457727025334E-5
4	0.0	0.0	0.0	0.0	6.846103027004453E-5
5	0.0	0.0	0.0	0.0	6.846103027004453E-5
6	0.0	0.0	0.0	0.0	1.1980680297257794E-4
7	0.0	0.0	0.0	0.0	1.1980680297257794E-4
8	0.0	0.0	0.0	0.0	1.3692206054008907E-4
9	0.0	0.0	0.0	5.13457727025334E-5	1.3692206054008907E-4
10	0.0	0.0	0.0	8.557628783755567E-5	1.3692206054008907E-4
11	0.0	0.0	0.0	1.1980680297257794E-4	1.3692206054008907E-4
12	0.0	0.0	0.0	1.1980680297257794E-4	2.3961360594515588E-4
13	0.0	0.0	0.0	1.1980680297257794E-4	3.2518989378271156E-4
14	0.0	0.0	0.0	1.1980680297257794E-4	3.594204089177338E-4
15	0.0	0.0	0.0	1.3692206054008907E-4	4.4499669675528944E-4
16	0.0	0.0	0.0	1.3692206054008907E-4	5.13457727025334E-4
17	0.0	0.0	0.0	1.3692206054008907E-4	5.305729845928452E-4
18	0.0	0.0	0.0	1.540373181076002E-4	5.648034997278674E-4
19	0.0	0.0	0.0	1.540373181076002E-4	5.648034997278674E-4
20	0.0	0.0	0.0	2.053830908101336E-4	6.674950451329342E-4
21	0.0	0.0	0.0	2.3961360594515588E-4	7.188408178354676E-4
22	0.0	0.0	0.0	3.765356664852449E-4	7.359560754029787E-4
23	0.0	0.0	0.0	5.476882421603563E-4	7.530713329704898E-4
24	0.0	0.0	0.0	6.674950451329342E-4	8.215323632405344E-4
25	0.0	0.0	0.0	7.359560754029787E-4	8.215323632405344E-4
26	0.0	0.0	0.0	0.0010611459691856904	8.728781359430678E-4
27	0.0	0.0	0.0	0.0013863358629684019	8.899933935105789E-4
28	0.0	0.0	0.0	0.0028924785289093815	8.899933935105789E-4
29	0.0	0.0	0.0	0.00664071993619432	8.899933935105789E-4
30	0.0	0.0	0.0	0.014154318008331708	8.899933935105789E-4
31	0.0	0.0	0.0	0.03284417927205387	9.071086510780901E-4
32	0.0	0.0	0.0	0.05220153558090896	9.413391662131123E-4
33	0.0	0.0	0.0	0.0721065801319244	9.413391662131123E-4
34	0.0	0.0	0.0	0.09464737434833657	0.001026915454050668
35	0.0	0.0	0.0	0.12059410482068345	0.001026915454050668
36	0.0	0.0	0.0	0.15843593930245056	0.001026915454050668
37	0.0	0.0	0.0	0.21719261853171629	0.001026915454050668
38	0.0	0.0	0.0	0.3032823640962973	0.0010611459691856904
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7035	0.0	27.519957	1
GGTATCA	3120	0.0	21.154163	1
TCAACGC	10205	0.0	18.689358	4
ATACCGT	1285	0.0	18.317774	6
AACGCAG	10450	0.0	18.272392	6
ATCAACG	10440	0.0	18.26867	3
CAACGCA	10475	0.0	18.186626	5
TATCAAC	10780	0.0	17.957607	2
CGTCGTA	1310	0.0	17.801031	10
TACCGTC	1305	0.0	17.194334	7
ACCGTCG	1300	0.0	17.091394	8
ATACGAA	1600	0.0	16.499823	40
CGCAATA	1630	0.0	16.197533	36
CCGTCGT	1380	0.0	15.941722	9
CGAATGC	1690	0.0	15.360389	43
CAATACG	1745	0.0	15.255373	38
ACGCAGA	12595	0.0	15.143165	7
TACGAAT	1745	0.0	15.128907	41
CGGATCG	365	0.0	15.069622	26
GTCGTAG	1505	0.0	14.909884	11
>>END_MODULE