FastQCFastQC Report
Thu 2 Feb 2017
SRR4063971_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063971_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015172
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27980.2756183188661626No Hit
GTCCTAAAGTGTGTATTTCTCATTT21560.212377803958344No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21200.20883160686070934No Hit
TATCAACGCAGAGTACTTTTTTTTT20000.1970109498685937No Hit
GTCCTACAGTGGACATTTCTAAATT19500.19208567612187885No Hit
CTTTAGGACGTGAAATATGGCGAGG17280.17021746068646496No Hit
CTGTAGGACGTGGAATATGGCAAGA17190.16933091141205628No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14280.1406658182061759No Hit
GTCCTACAGTGTGCATTTCTCATTT13610.134065951385578No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12720.1252989641164256No Hit
CTGTAGGACCTGGAATATGGCGAGA10690.10530235270476333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA551.12692815E-515.5463419
CGAGACT508.730601E-515.1956254
ACGAACG456.746447E-414.78153515
AATACGA456.7742367E-414.7735245
TCCAACG1500.013.93687518
CGTCTTA551.9548752E-413.82169515
ATAACAC551.9647076E-413.8135243
GCGTAAC707.248631E-613.57287211
GGTCGCG707.2634084E-613.5701977
CCAACGA1001.0018994E-813.30338219
CGCGTAA655.4434153E-513.15524510
CCCTTAA604.099795E-412.6630211
GTATAGA905.4176417E-712.6630211
TTTTCGG1001.4357283E-712.3531416
GCCGGTT853.9348215E-612.29542511
GGGTCGC853.9500283E-612.2911846
GTGTAAG701.09311455E-412.2107711
CGGACCA1650.012.09159859
CTCTTAC550.003073803812.0874281
ATTAGAC550.003074938412.0868343