##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063971_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1015172
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.165912771431834	32.0	32.0	32.0	32.0	32.0
2	30.730397410488074	32.0	32.0	32.0	32.0	32.0
3	30.75827150473023	32.0	32.0	32.0	32.0	32.0
4	30.777055513745452	32.0	32.0	32.0	32.0	32.0
5	30.691612849842194	32.0	32.0	32.0	32.0	32.0
6	34.33466151548703	36.0	36.0	36.0	32.0	36.0
7	34.25969490884303	36.0	36.0	36.0	32.0	36.0
8	34.20775297191018	36.0	36.0	36.0	32.0	36.0
9	34.34219028893626	36.0	36.0	36.0	32.0	36.0
10	34.021380613334486	36.0	36.0	36.0	32.0	36.0
11	34.355616585169805	36.0	36.0	36.0	32.0	36.0
12	34.13641629201751	36.0	36.0	36.0	32.0	36.0
13	34.22454815538648	36.0	36.0	36.0	32.0	36.0
14	34.12170055911707	36.0	36.0	36.0	32.0	36.0
15	34.07714160753055	36.0	36.0	36.0	32.0	36.0
16	34.07434700720666	36.0	36.0	36.0	32.0	36.0
17	34.00248923335159	36.0	36.0	36.0	32.0	36.0
18	34.00012214678892	36.0	36.0	36.0	32.0	36.0
19	33.99571205667611	36.0	36.0	36.0	32.0	36.0
20	33.96392433991481	36.0	36.0	36.0	32.0	36.0
21	33.958735071495276	36.0	36.0	36.0	32.0	36.0
22	33.92451919477685	36.0	36.0	36.0	32.0	36.0
23	33.89218477262966	36.0	36.0	36.0	32.0	36.0
24	33.87727005866986	36.0	36.0	36.0	32.0	36.0
25	33.4903257773067	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	1.0
4	8.0
5	10.0
6	35.0
7	22.0
8	51.0
9	59.0
10	85.0
11	28.0
12	64.0
13	57.0
14	91.0
15	185.0
16	284.0
17	404.0
18	557.0
19	780.0
20	1186.0
21	1745.0
22	2880.0
23	4467.0
24	6349.0
25	9237.0
26	12978.0
27	16690.0
28	22802.0
29	29877.0
30	38687.0
31	51914.0
32	71954.0
33	99631.0
34	208129.0
35	433924.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.2890317520068	17.490702619821406	11.293730205498216	25.926535422673584
2	15.459673415347272	19.971983387054827	40.16975466746526	24.398588530132635
3	19.074511333670223	24.800007092471045	28.70086616802655	27.424615405832174
4	12.063128051909928	16.046507733393227	36.95075472125602	34.93960949344083
5	13.490842245462497	37.67752015572911	34.48649191800578	14.345145680802604
6	32.843936011538254	36.3378250306879	17.76824575541496	13.04999320235888
7	29.255825410118725	30.687127444701712	21.465195329819203	18.591851815360364
8	26.686807653575023	35.35738427308183	19.751765292468022	18.204042780875128
9	27.56077081818128	14.122304412869044	19.03551148402407	39.281413284925605
10	15.64208808235002	27.471328489369167	32.75727481677436	24.129308611506453
11	36.27007656098465	20.87834215111924	24.33413232198191	18.5174489659142
12	25.124022335355605	23.52861162995855	30.54473231578999	20.802633718895848
13	30.055109642659655	20.445864706862242	25.54027853483854	23.958747115639568
14	22.47396015585957	21.014132042980652	26.132500347460773	30.379407453699002
15	23.838130758585887	29.848645537231462	22.735498318502778	23.577725385679873
16	23.65605282638717	26.13581365273097	26.78786349437601	23.42027002650585
17	22.403586277049364	27.050350210047174	26.994955319388016	23.551108193515446
18	23.045171339563865	25.538171852202375	29.15739973973737	22.259257068496392
19	25.35767625037584	24.791723145323076	26.11428486930634	23.73631573499475
20	25.341968099736878	25.536279919436012	26.315005979204397	22.806746001622717
21	25.36097088278292	24.715772704913267	25.22029895003731	24.702957462266504
22	25.180383786901167	26.203191159000948	25.671134157822873	22.945290896275008
23	23.334641275499944	26.168767918479052	26.677780888796	23.818809917225007
24	24.47080975571285	25.51607878704233	26.960409314063767	23.052702143181055
25	23.970444709524138	25.887496672811693	27.044766702485283	23.09729191517888
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	143.0
1	143.0
2	370.5
3	598.0
4	598.0
5	598.0
6	1667.0
7	2736.0
8	2736.0
9	2736.0
10	2728.5
11	2721.0
12	2721.0
13	2721.0
14	2804.0
15	2887.0
16	2887.0
17	2887.0
18	5286.0
19	7685.0
20	7685.0
21	7685.0
22	12920.0
23	18155.0
24	18155.0
25	18155.0
26	28441.5
27	38728.0
28	38728.0
29	38728.0
30	50866.0
31	63004.0
32	63004.0
33	63004.0
34	71391.0
35	79778.0
36	79778.0
37	79778.0
38	86657.5
39	93537.0
40	93537.0
41	93537.0
42	103345.0
43	113153.0
44	113153.0
45	113153.0
46	123084.5
47	133016.0
48	133016.0
49	133016.0
50	131427.5
51	129839.0
52	129839.0
53	129839.0
54	117761.0
55	105683.0
56	105683.0
57	105683.0
58	94789.5
59	83896.0
60	83896.0
61	83896.0
62	72888.0
63	61880.0
64	61880.0
65	61880.0
66	50248.0
67	38616.0
68	38616.0
69	38616.0
70	28940.0
71	19264.0
72	19264.0
73	19264.0
74	14525.0
75	9786.0
76	9786.0
77	9786.0
78	7654.5
79	5523.0
80	5523.0
81	5523.0
82	3832.0
83	2141.0
84	2141.0
85	2141.0
86	1646.5
87	1152.0
88	1152.0
89	1152.0
90	814.5
91	477.0
92	477.0
93	477.0
94	339.5
95	202.0
96	202.0
97	202.0
98	387.0
99	572.0
100	572.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036447025725689836
2	0.005910328496057811
3	0.0010835602242772653
4	0.0028566587730946085
5	0.007486416095006561
6	0.01122962414250984
7	0.0218682154354139
8	0.03211278482858077
9	0.045115507519907956
10	0.05673915356215498
11	0.05476904406346905
12	0.0627479875331471
13	0.06393005323235866
14	0.06590016273104458
15	0.059103284960578116
16	0.06688521748038756
17	0.06284649300808139
18	0.07998644564664904
19	0.07653875402394865
20	0.0810700058709263
21	0.07437163357539411
22	0.07949391827197755
23	0.08432068654375811
24	0.0780163361479631
25	0.07900139089730608
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1015172.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.753606398487506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.00952354721406	42.16549141239215
2	13.771346196147451	15.080617384023315
3	4.179906483136588	6.865948630804408
4	1.7614817166177792	3.8578990635928796
5	0.8611877715735738	2.3576568139965013
6	0.552060951465288	1.8136396826702939
7	0.3399450081255404	1.3029250620425534
8	0.2451577367205971	1.0738616177554883
9	0.18062167840121596	0.8900719457592934
>10	0.9209933956944858	9.319296066912548
>50	0.09567747425489334	3.6434850688244986
>100	0.07522639880700817	7.921389389033672
>500	0.004882482361135666	1.766975537925211
>1k	0.0019891594804626786	1.9407423242671782
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2798	0.2756183188661626	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2156	0.212377803958344	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2120	0.20883160686070934	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2000	0.1970109498685937	No Hit
GTCCTACAGTGGACATTTCTAAATT	1950	0.19208567612187885	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1728	0.17021746068646496	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1719	0.16933091141205628	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1428	0.1406658182061759	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1361	0.134065951385578	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1272	0.1252989641164256	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1069	0.10530235270476333	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	9.850547493429685E-5	0.0
6	0.0	0.0	0.0	9.850547493429685E-5	0.0
7	0.0	0.0	0.0	9.850547493429685E-5	0.0
8	0.0	0.0	0.0	2.9551642480289057E-4	0.0
9	0.0	0.0	0.0	3.940218997371874E-4	0.0
10	0.0	0.0	0.0	4.925273746714842E-4	0.0
11	0.0	0.0	0.0	4.925273746714842E-4	0.0
12	0.0	0.0	0.0	5.910328496057811E-4	0.0
13	0.0	0.0	0.0	5.910328496057811E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTA	55	1.12692815E-5	15.546341	9
CGAGACT	50	8.730601E-5	15.195625	4
ACGAACG	45	6.746447E-4	14.781535	15
AATACGA	45	6.7742367E-4	14.773524	5
TCCAACG	150	0.0	13.936875	18
CGTCTTA	55	1.9548752E-4	13.821695	15
ATAACAC	55	1.9647076E-4	13.813524	3
GCGTAAC	70	7.248631E-6	13.572872	11
GGTCGCG	70	7.2634084E-6	13.570197	7
CCAACGA	100	1.0018994E-8	13.303382	19
CGCGTAA	65	5.4434153E-5	13.155245	10
CCCTTAA	60	4.099795E-4	12.663021	1
GTATAGA	90	5.4176417E-7	12.663021	1
TTTTCGG	100	1.4357283E-7	12.35314	16
GCCGGTT	85	3.9348215E-6	12.295425	11
GGGTCGC	85	3.9500283E-6	12.291184	6
GTGTAAG	70	1.09311455E-4	12.210771	1
CGGACCA	165	0.0	12.0915985	9
CTCTTAC	55	0.0030738038	12.087428	1
ATTAGAC	55	0.0030749384	12.086834	3
>>END_MODULE